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Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones

This study describes the application of a simple and effective method to isolate SSR markers from oil palm genomic sequences. A total of 12 informative SSR markers are described. The SSR markers were found suitable for genome analysis and DNA fingerprinting of oil palm tissue culture clones. Eleven...

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Main Authors: Singh, Rajinder, Nagappan, Jayanthi, Tan, Soon Guan, Panandam, Jothi Malar, Cheah, Suan Choo
Format: Article
Language:English
Published: Malaysian Society for Molecular Biology and Biotechnology 2007
Online Access:http://psasir.upm.edu.my/id/eprint/58512/1/Development%20of%20simple%20sequence%20repeat%20%28SSR%29%20markers%20for%20oil%20palm%20and%20their%20application%20in%20genetic%20mapping%20and%20fingerprinting%20of%20tissue%20culture%20clones.pdf
http://psasir.upm.edu.my/id/eprint/58512/
http://www.msmbb.org.my/apjmbb/html153/153d.htm
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spelling my.upm.eprints.585122018-01-19T09:53:03Z http://psasir.upm.edu.my/id/eprint/58512/ Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones Singh, Rajinder Nagappan, Jayanthi Tan, Soon Guan Panandam, Jothi Malar Cheah, Suan Choo This study describes the application of a simple and effective method to isolate SSR markers from oil palm genomic sequences. A total of 12 informative SSR markers are described. The SSR markers were found suitable for genome analysis and DNA fingerprinting of oil palm tissue culture clones. Eleven of the 12 SSR markers exhibited expected Mendelian segregation ratios when tested on a mapping population, indicating their suitability for genetic mapping studies. The markers identified in the species Elaeis oleifera also showed applicability in a second species, that is Elaeis guineensis. Apart from genetic mapping, the SSR markers also showed promise as molecular probes for DNA fingerprinting of oil palm tissue culture clones. The SSR markers can be used for clonal identification, monitoring line uniformity between and within clones and detecting culture mix-up. Malaysian Society for Molecular Biology and Biotechnology 2007 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58512/1/Development%20of%20simple%20sequence%20repeat%20%28SSR%29%20markers%20for%20oil%20palm%20and%20their%20application%20in%20genetic%20mapping%20and%20fingerprinting%20of%20tissue%20culture%20clones.pdf Singh, Rajinder and Nagappan, Jayanthi and Tan, Soon Guan and Panandam, Jothi Malar and Cheah, Suan Choo (2007) Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones. Asia Pacific Journal of Molecular Biology and Biotechnology, 15 (3). pp. 121-131. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html153/153d.htm
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This study describes the application of a simple and effective method to isolate SSR markers from oil palm genomic sequences. A total of 12 informative SSR markers are described. The SSR markers were found suitable for genome analysis and DNA fingerprinting of oil palm tissue culture clones. Eleven of the 12 SSR markers exhibited expected Mendelian segregation ratios when tested on a mapping population, indicating their suitability for genetic mapping studies. The markers identified in the species Elaeis oleifera also showed applicability in a second species, that is Elaeis guineensis. Apart from genetic mapping, the SSR markers also showed promise as molecular probes for DNA fingerprinting of oil palm tissue culture clones. The SSR markers can be used for clonal identification, monitoring line uniformity between and within clones and detecting culture mix-up.
format Article
author Singh, Rajinder
Nagappan, Jayanthi
Tan, Soon Guan
Panandam, Jothi Malar
Cheah, Suan Choo
spellingShingle Singh, Rajinder
Nagappan, Jayanthi
Tan, Soon Guan
Panandam, Jothi Malar
Cheah, Suan Choo
Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones
author_facet Singh, Rajinder
Nagappan, Jayanthi
Tan, Soon Guan
Panandam, Jothi Malar
Cheah, Suan Choo
author_sort Singh, Rajinder
title Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones
title_short Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones
title_full Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones
title_fullStr Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones
title_full_unstemmed Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones
title_sort development of simple sequence repeat (ssr) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones
publisher Malaysian Society for Molecular Biology and Biotechnology
publishDate 2007
url http://psasir.upm.edu.my/id/eprint/58512/1/Development%20of%20simple%20sequence%20repeat%20%28SSR%29%20markers%20for%20oil%20palm%20and%20their%20application%20in%20genetic%20mapping%20and%20fingerprinting%20of%20tissue%20culture%20clones.pdf
http://psasir.upm.edu.my/id/eprint/58512/
http://www.msmbb.org.my/apjmbb/html153/153d.htm
_version_ 1643836805819138048
score 13.211869

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