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Saccharomyces cerevisiae kinetochore protein (rDsn1p) induced apoptosis in Chinese hamster ovary cells

Dsn1p is a member of the MIND complex that forms part of the yeast kinetochore, which is essential for the proper chromosomal segregation during cell division. Its functionality is gene dosage dependent and it has characteristics of haploinsufficiency. Bioinformatics alignments predicted the existen...

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Main Authors: Yiap, Beow Chin, Abdul Rahim, Raha, G. K. Radhakrishnan, Ammu Kutty
Format: Article
Language:English
Published: Malaysian Society for Molecular Biology and Biotechnology 2008
Online Access:http://psasir.upm.edu.my/id/eprint/58502/1/Saccharomyces%20cerevisiae%20kinetochore%20protein%20%28rDsn1p%29%20induced%20apoptosis%20in%20Chinese%20hamster%20ovary%20cells.pdf
http://psasir.upm.edu.my/id/eprint/58502/
http://www.msmbb.org.my/apjmbb/html163/163c.htm
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spelling my.upm.eprints.585022018-01-19T09:03:35Z http://psasir.upm.edu.my/id/eprint/58502/ Saccharomyces cerevisiae kinetochore protein (rDsn1p) induced apoptosis in Chinese hamster ovary cells Yiap, Beow Chin Abdul Rahim, Raha G. K. Radhakrishnan, Ammu Kutty Dsn1p is a member of the MIND complex that forms part of the yeast kinetochore, which is essential for the proper chromosomal segregation during cell division. Its functionality is gene dosage dependent and it has characteristics of haploinsufficiency. Bioinformatics alignments predicted the existence of nuclear homologues in higher eukaryotic organisms. Literature on the possibility of Dsn1p being a functional homologue of these organisms is scarce. In this study we employed recombinant DNA expression technology to explore whether Dsn1p can function in a mammalian cell line, Chinese Hamster Ovary (CHO). Expression of rDsn1p in CHO cells induced cytopathic effects including changes in cellular morphology and cell size. Inhibition of cell growth was observed at the beginning the fourth post-transfection week. The recombinant CHO cell culture showed cytotoxic effects following the accumulation of the Dsn1p, resulting in apoptotic cell death; as evidenced by the presence of nuclear fragmentation and surface blebbing in the dying cells. This suggests that rDsn1p may interact with the counterpart/ligand of the nuclear homologue of this protein in CHO cells, resulting in nuclear anomalies and inhibition of cell growth, as observed in our previous study using yeast cells. Malaysian Society for Molecular Biology and Biotechnology 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58502/1/Saccharomyces%20cerevisiae%20kinetochore%20protein%20%28rDsn1p%29%20induced%20apoptosis%20in%20Chinese%20hamster%20ovary%20cells.pdf Yiap, Beow Chin and Abdul Rahim, Raha and G. K. Radhakrishnan, Ammu Kutty (2008) Saccharomyces cerevisiae kinetochore protein (rDsn1p) induced apoptosis in Chinese hamster ovary cells. Asia Pacific Journal of Molecular Biology and Biotechnology, 16 (3). pp. 71-77. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html163/163c.htm
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Dsn1p is a member of the MIND complex that forms part of the yeast kinetochore, which is essential for the proper chromosomal segregation during cell division. Its functionality is gene dosage dependent and it has characteristics of haploinsufficiency. Bioinformatics alignments predicted the existence of nuclear homologues in higher eukaryotic organisms. Literature on the possibility of Dsn1p being a functional homologue of these organisms is scarce. In this study we employed recombinant DNA expression technology to explore whether Dsn1p can function in a mammalian cell line, Chinese Hamster Ovary (CHO). Expression of rDsn1p in CHO cells induced cytopathic effects including changes in cellular morphology and cell size. Inhibition of cell growth was observed at the beginning the fourth post-transfection week. The recombinant CHO cell culture showed cytotoxic effects following the accumulation of the Dsn1p, resulting in apoptotic cell death; as evidenced by the presence of nuclear fragmentation and surface blebbing in the dying cells. This suggests that rDsn1p may interact with the counterpart/ligand of the nuclear homologue of this protein in CHO cells, resulting in nuclear anomalies and inhibition of cell growth, as observed in our previous study using yeast cells.
format Article
author Yiap, Beow Chin
Abdul Rahim, Raha
G. K. Radhakrishnan, Ammu Kutty
spellingShingle Yiap, Beow Chin
Abdul Rahim, Raha
G. K. Radhakrishnan, Ammu Kutty
Saccharomyces cerevisiae kinetochore protein (rDsn1p) induced apoptosis in Chinese hamster ovary cells
author_facet Yiap, Beow Chin
Abdul Rahim, Raha
G. K. Radhakrishnan, Ammu Kutty
author_sort Yiap, Beow Chin
title Saccharomyces cerevisiae kinetochore protein (rDsn1p) induced apoptosis in Chinese hamster ovary cells
title_short Saccharomyces cerevisiae kinetochore protein (rDsn1p) induced apoptosis in Chinese hamster ovary cells
title_full Saccharomyces cerevisiae kinetochore protein (rDsn1p) induced apoptosis in Chinese hamster ovary cells
title_fullStr Saccharomyces cerevisiae kinetochore protein (rDsn1p) induced apoptosis in Chinese hamster ovary cells
title_full_unstemmed Saccharomyces cerevisiae kinetochore protein (rDsn1p) induced apoptosis in Chinese hamster ovary cells
title_sort saccharomyces cerevisiae kinetochore protein (rdsn1p) induced apoptosis in chinese hamster ovary cells
publisher Malaysian Society for Molecular Biology and Biotechnology
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/58502/1/Saccharomyces%20cerevisiae%20kinetochore%20protein%20%28rDsn1p%29%20induced%20apoptosis%20in%20Chinese%20hamster%20ovary%20cells.pdf
http://psasir.upm.edu.my/id/eprint/58502/
http://www.msmbb.org.my/apjmbb/html163/163c.htm
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score 13.211869

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