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Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato

A gene LN2-1-1 identified by screening a tomato pistil cDNA library has been found to encode a basic protein containing ankryin repeats. This clone was isolated by its binding ability to a specific DNA fragment in the tomato floral chitinase promoter using a yeast one hybrid system. An analysis of t...

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Main Authors: Chan, Pick Kuen, Mohd Yusuf, Hirzun, Wong, Clemente Michael Vui Ling, Kulaveerasingam, Harikrishna
Format: Article
Language:English
Published: Malaysian Society for Molecular Biology and Biotechnology 2006
Online Access:http://psasir.upm.edu.my/id/eprint/58494/1/Isolation%20and%20characterization%20of%20a%20novel%20pistil%20predominant%20gene%20that%20binds%20weakly%20to%20the%20chitinase.pdf
http://psasir.upm.edu.my/id/eprint/58494/
http://www.msmbb.org.my/apjmbb/html141/141c.htm
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spelling my.upm.eprints.584942018-01-18T10:08:49Z http://psasir.upm.edu.my/id/eprint/58494/ Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato Chan, Pick Kuen Mohd Yusuf, Hirzun Wong, Clemente Michael Vui Ling Kulaveerasingam, Harikrishna A gene LN2-1-1 identified by screening a tomato pistil cDNA library has been found to encode a basic protein containing ankryin repeats. This clone was isolated by its binding ability to a specific DNA fragment in the tomato floral chitinase promoter using a yeast one hybrid system. An analysis of the temporal and spatial patterns of gene expression through northern blotting has demonstrated the highest level of expression in mature tomato pistils. In situ hybridization revealed that the mRNA was maximal on the upper half of the style and decreased in a gradient from the top to the lower half of the style. Hybridization was also detected on the stigma, ovules and ovary walls. The LN2-1-1 could possibly play a role in modulating the defense mechanism of tomato flowers based on its ability to bind to the chitinase, Chi 2;1 promoter. Malaysian Society for Molecular Biology and Biotechnology 2006 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58494/1/Isolation%20and%20characterization%20of%20a%20novel%20pistil%20predominant%20gene%20that%20binds%20weakly%20to%20the%20chitinase.pdf Chan, Pick Kuen and Mohd Yusuf, Hirzun and Wong, Clemente Michael Vui Ling and Kulaveerasingam, Harikrishna (2006) Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato. Asia Pacific Journal of Molecular Biology and Biotechnology, 14 (1). pp. 19-26. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html141/141c.htm
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A gene LN2-1-1 identified by screening a tomato pistil cDNA library has been found to encode a basic protein containing ankryin repeats. This clone was isolated by its binding ability to a specific DNA fragment in the tomato floral chitinase promoter using a yeast one hybrid system. An analysis of the temporal and spatial patterns of gene expression through northern blotting has demonstrated the highest level of expression in mature tomato pistils. In situ hybridization revealed that the mRNA was maximal on the upper half of the style and decreased in a gradient from the top to the lower half of the style. Hybridization was also detected on the stigma, ovules and ovary walls. The LN2-1-1 could possibly play a role in modulating the defense mechanism of tomato flowers based on its ability to bind to the chitinase, Chi 2;1 promoter.
format Article
author Chan, Pick Kuen
Mohd Yusuf, Hirzun
Wong, Clemente Michael Vui Ling
Kulaveerasingam, Harikrishna
spellingShingle Chan, Pick Kuen
Mohd Yusuf, Hirzun
Wong, Clemente Michael Vui Ling
Kulaveerasingam, Harikrishna
Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato
author_facet Chan, Pick Kuen
Mohd Yusuf, Hirzun
Wong, Clemente Michael Vui Ling
Kulaveerasingam, Harikrishna
author_sort Chan, Pick Kuen
title Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato
title_short Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato
title_full Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato
title_fullStr Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato
title_full_unstemmed Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato
title_sort isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, chi2;1 promoter of tomato
publisher Malaysian Society for Molecular Biology and Biotechnology
publishDate 2006
url http://psasir.upm.edu.my/id/eprint/58494/1/Isolation%20and%20characterization%20of%20a%20novel%20pistil%20predominant%20gene%20that%20binds%20weakly%20to%20the%20chitinase.pdf
http://psasir.upm.edu.my/id/eprint/58494/
http://www.msmbb.org.my/apjmbb/html141/141c.htm
_version_ 1643836800452526080
score 13.211869

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