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Anti-proliferation of MDA-MB-231 cells by Averrhoa bilimbi extract is associated with G0/G1 perturbation and mitochondria-mediated apoptosis independent of p53

Practiced as folk medicine since ancient times, bilimbi (Averrhoa bilimbi) is commonly consumed and widely cultivated in Malaysia. In search for naturally occurring anticancer agents, a potential fruit extract was found to exert anticancer properties in vitro without any cytotoxic effect on normal c...

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Main Authors: Yan, See Wan, Rahmat, Asmah
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2017
Online Access:http://psasir.upm.edu.my/id/eprint/58363/1/%2859%29.pdf
http://psasir.upm.edu.my/id/eprint/58363/
http://www.ifrj.upm.edu.my/24%20(03)%202017/(59).pdf
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spelling my.upm.eprints.583632018-01-12T04:44:58Z http://psasir.upm.edu.my/id/eprint/58363/ Anti-proliferation of MDA-MB-231 cells by Averrhoa bilimbi extract is associated with G0/G1 perturbation and mitochondria-mediated apoptosis independent of p53 Yan, See Wan Rahmat, Asmah Practiced as folk medicine since ancient times, bilimbi (Averrhoa bilimbi) is commonly consumed and widely cultivated in Malaysia. In search for naturally occurring anticancer agents, a potential fruit extract was found to exert anticancer properties in vitro without any cytotoxic effect on normal cells. This study investigated the anti-proliferative effect and underlying cell death pathway induced by bilimbi ethanolic extract on human non-hormone-dependent breast cancer cell line, MDA-MB-231. Anticancer potential of bilimbi extract was conducted by investigating the in vitro growth inhibitory effect, DNA fragmentation, cell cycle progression and anti-proliferation assay. Release of caspases, cytochrome c and apoptotic proteins were demonstrated to determine the mechanism of cell death pathway. Findings revealed that bilimbi inhibited growth of MDA-MB-231 cells through the induction of apoptosis mediated by cell cycle arrest at G0/G1 checkpoint. Released of cytochrome c coupled with up-regulation of caspase-3/7, caspase-9 and Bax pro-apoptotic proteins in addition to down-regulation of dysfunctional p53 and Bcl-2 anti-apoptotic proteins implied that bilimbi induced a p53-independent mitochondrial apoptosis pathway in MDA-MB-231. These results suggest that bilimbi could induce tumor cell anti-proliferation through apoptosis. As a natural product, bilimbi could be a promising anticancer agent and an inexpensive approach to cancer chemoprevention strategy. Faculty of Food Science and Technology, Universiti Putra Malaysia 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58363/1/%2859%29.pdf Yan, See Wan and Rahmat, Asmah (2017) Anti-proliferation of MDA-MB-231 cells by Averrhoa bilimbi extract is associated with G0/G1 perturbation and mitochondria-mediated apoptosis independent of p53. International Food Research Journal, 24 (3). pp. 1331-1337. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/24%20(03)%202017/(59).pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Practiced as folk medicine since ancient times, bilimbi (Averrhoa bilimbi) is commonly consumed and widely cultivated in Malaysia. In search for naturally occurring anticancer agents, a potential fruit extract was found to exert anticancer properties in vitro without any cytotoxic effect on normal cells. This study investigated the anti-proliferative effect and underlying cell death pathway induced by bilimbi ethanolic extract on human non-hormone-dependent breast cancer cell line, MDA-MB-231. Anticancer potential of bilimbi extract was conducted by investigating the in vitro growth inhibitory effect, DNA fragmentation, cell cycle progression and anti-proliferation assay. Release of caspases, cytochrome c and apoptotic proteins were demonstrated to determine the mechanism of cell death pathway. Findings revealed that bilimbi inhibited growth of MDA-MB-231 cells through the induction of apoptosis mediated by cell cycle arrest at G0/G1 checkpoint. Released of cytochrome c coupled with up-regulation of caspase-3/7, caspase-9 and Bax pro-apoptotic proteins in addition to down-regulation of dysfunctional p53 and Bcl-2 anti-apoptotic proteins implied that bilimbi induced a p53-independent mitochondrial apoptosis pathway in MDA-MB-231. These results suggest that bilimbi could induce tumor cell anti-proliferation through apoptosis. As a natural product, bilimbi could be a promising anticancer agent and an inexpensive approach to cancer chemoprevention strategy.
format Article
author Yan, See Wan
Rahmat, Asmah
spellingShingle Yan, See Wan
Rahmat, Asmah
Anti-proliferation of MDA-MB-231 cells by Averrhoa bilimbi extract is associated with G0/G1 perturbation and mitochondria-mediated apoptosis independent of p53
author_facet Yan, See Wan
Rahmat, Asmah
author_sort Yan, See Wan
title Anti-proliferation of MDA-MB-231 cells by Averrhoa bilimbi extract is associated with G0/G1 perturbation and mitochondria-mediated apoptosis independent of p53
title_short Anti-proliferation of MDA-MB-231 cells by Averrhoa bilimbi extract is associated with G0/G1 perturbation and mitochondria-mediated apoptosis independent of p53
title_full Anti-proliferation of MDA-MB-231 cells by Averrhoa bilimbi extract is associated with G0/G1 perturbation and mitochondria-mediated apoptosis independent of p53
title_fullStr Anti-proliferation of MDA-MB-231 cells by Averrhoa bilimbi extract is associated with G0/G1 perturbation and mitochondria-mediated apoptosis independent of p53
title_full_unstemmed Anti-proliferation of MDA-MB-231 cells by Averrhoa bilimbi extract is associated with G0/G1 perturbation and mitochondria-mediated apoptosis independent of p53
title_sort anti-proliferation of mda-mb-231 cells by averrhoa bilimbi extract is associated with g0/g1 perturbation and mitochondria-mediated apoptosis independent of p53
publisher Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/58363/1/%2859%29.pdf
http://psasir.upm.edu.my/id/eprint/58363/
http://www.ifrj.upm.edu.my/24%20(03)%202017/(59).pdf
_version_ 1643836761559793664
score 13.211869

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