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Isolation and characterization of genotype VII Newcastle disease virus from NDV vaccinated farms in Malaysia

Molecular analysis, particularly sub-genotype classification, and study on the relationship of recent Malaysian NDVs with other isolates from around the world are lacking. Therefore, in the present study, a molecular epidemiological investigation was conducted to characterise six Newcastle disease v...

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Main Authors: Aljumaili, O. A., Yeap, Swee Keong, Omar, Abdul Rahman, Ideris, Aini
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2017
Online Access:http://psasir.upm.edu.my/id/eprint/58348/1/19%20JTAS%20Vol%2040%20%284%29%20Nov%202017_1125-2017_pg677-690.pdf
http://psasir.upm.edu.my/id/eprint/58348/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2040%20(4)%20Nov.%202017/19%20JTAS%20Vol%2040%20(4)%20Nov%202017_1125-2017_pg677-690.pdf
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spelling my.upm.eprints.583482018-01-25T09:44:20Z http://psasir.upm.edu.my/id/eprint/58348/ Isolation and characterization of genotype VII Newcastle disease virus from NDV vaccinated farms in Malaysia Aljumaili, O. A. Yeap, Swee Keong Omar, Abdul Rahman Ideris, Aini Molecular analysis, particularly sub-genotype classification, and study on the relationship of recent Malaysian NDVs with other isolates from around the world are lacking. Therefore, in the present study, a molecular epidemiological investigation was conducted to characterise six Newcastle disease viruses (NDV) isolated between 2014 and 2015 from vaccinated commercial poultry flocks. Partial Fusion (F) and Hemagglutinin-neuraminidase (HN) genes were amplified from IBS046/2014, IBS060/2014, IBS061/2014, IBS074/2014, IBS160/2015, and IBS162A/2015 isolates using one-step reverse transcription polymerase chain reaction (RT-PCR), sequenced and phylogenetically analysed. Sequence and phylogenetic analysis revealed that all the recently isolated strains of NDV belonged to sub-genotype VIIa and lineage 5a. Moreover, deduced amino acid sequence at the F protein cleavage site of the isolates revealed either 112RRQKRF117 or 112KRRKRF117 consistent with the motif found in velogenic pathotypes. The study concluded that the genotype VIIa was the causative agent of recent ND outbreaks in vaccinated broiler flocks from Malaysia. Interestingly, five out of the six isolates characterised in this study had a unique F0 protein cleavage site (112KRRKRF117). Further studies are required to determine the role of these motifs on the virulent potential of the isolates. Universiti Putra Malaysia Press 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58348/1/19%20JTAS%20Vol%2040%20%284%29%20Nov%202017_1125-2017_pg677-690.pdf Aljumaili, O. A. and Yeap, Swee Keong and Omar, Abdul Rahman and Ideris, Aini (2017) Isolation and characterization of genotype VII Newcastle disease virus from NDV vaccinated farms in Malaysia. Pertanika Journal of Tropical Agricultural Science, 40 (4). pp. 677-690. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2040%20(4)%20Nov.%202017/19%20JTAS%20Vol%2040%20(4)%20Nov%202017_1125-2017_pg677-690.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Molecular analysis, particularly sub-genotype classification, and study on the relationship of recent Malaysian NDVs with other isolates from around the world are lacking. Therefore, in the present study, a molecular epidemiological investigation was conducted to characterise six Newcastle disease viruses (NDV) isolated between 2014 and 2015 from vaccinated commercial poultry flocks. Partial Fusion (F) and Hemagglutinin-neuraminidase (HN) genes were amplified from IBS046/2014, IBS060/2014, IBS061/2014, IBS074/2014, IBS160/2015, and IBS162A/2015 isolates using one-step reverse transcription polymerase chain reaction (RT-PCR), sequenced and phylogenetically analysed. Sequence and phylogenetic analysis revealed that all the recently isolated strains of NDV belonged to sub-genotype VIIa and lineage 5a. Moreover, deduced amino acid sequence at the F protein cleavage site of the isolates revealed either 112RRQKRF117 or 112KRRKRF117 consistent with the motif found in velogenic pathotypes. The study concluded that the genotype VIIa was the causative agent of recent ND outbreaks in vaccinated broiler flocks from Malaysia. Interestingly, five out of the six isolates characterised in this study had a unique F0 protein cleavage site (112KRRKRF117). Further studies are required to determine the role of these motifs on the virulent potential of the isolates.
format Article
author Aljumaili, O. A.
Yeap, Swee Keong
Omar, Abdul Rahman
Ideris, Aini
spellingShingle Aljumaili, O. A.
Yeap, Swee Keong
Omar, Abdul Rahman
Ideris, Aini
Isolation and characterization of genotype VII Newcastle disease virus from NDV vaccinated farms in Malaysia
author_facet Aljumaili, O. A.
Yeap, Swee Keong
Omar, Abdul Rahman
Ideris, Aini
author_sort Aljumaili, O. A.
title Isolation and characterization of genotype VII Newcastle disease virus from NDV vaccinated farms in Malaysia
title_short Isolation and characterization of genotype VII Newcastle disease virus from NDV vaccinated farms in Malaysia
title_full Isolation and characterization of genotype VII Newcastle disease virus from NDV vaccinated farms in Malaysia
title_fullStr Isolation and characterization of genotype VII Newcastle disease virus from NDV vaccinated farms in Malaysia
title_full_unstemmed Isolation and characterization of genotype VII Newcastle disease virus from NDV vaccinated farms in Malaysia
title_sort isolation and characterization of genotype vii newcastle disease virus from ndv vaccinated farms in malaysia
publisher Universiti Putra Malaysia Press
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/58348/1/19%20JTAS%20Vol%2040%20%284%29%20Nov%202017_1125-2017_pg677-690.pdf
http://psasir.upm.edu.my/id/eprint/58348/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2040%20(4)%20Nov.%202017/19%20JTAS%20Vol%2040%20(4)%20Nov%202017_1125-2017_pg677-690.pdf
_version_ 1643836757435744256
score 13.244745

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