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Phylogenetic grouping and virulence gene profiles of Escherichia coli isolated from chicken

Colibacillosis is a disease caused by avian pathogenic E. coli (APEC) and is one of the principle cause of morbidity and mortality in poultry worldwide which is represented by a complex syndrome characterized by multiple organ lesions. This study was carried out to determine phylogenetic grouping an...

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Main Authors: Roslee, Roseliza, Bejo, Siti Khairani, Zakaria, Zunita, M., Ramlan, Khoo, Evie
Format: Article
Language:English
Published: Department of Veterinary Services Malaysia 2017
Online Access:http://psasir.upm.edu.my/id/eprint/56570/1/Phylogenetic%20grouping%20and%20virulence%20gene%20profiles%20of%20Escherichia%20coli%20isolated%20from%20chicken.pdf
http://psasir.upm.edu.my/id/eprint/56570/
http://www.dvs.gov.my/index.php/pages/view/1663
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spelling my.upm.eprints.565702017-08-03T04:49:44Z http://psasir.upm.edu.my/id/eprint/56570/ Phylogenetic grouping and virulence gene profiles of Escherichia coli isolated from chicken Roslee, Roseliza Bejo, Siti Khairani Zakaria, Zunita M., Ramlan Khoo, Evie Colibacillosis is a disease caused by avian pathogenic E. coli (APEC) and is one of the principle cause of morbidity and mortality in poultry worldwide which is represented by a complex syndrome characterized by multiple organ lesions. This study was carried out to determine phylogenetic grouping and virulence associated genes contained by E. coli isolates which is related in causing disease in chicken. E. coli isolates obtained from clinical cases of Veterinary Research Institute were re-identified by conventional methods. Phylogenetic grouping of the isolates was determined by triplex polymerase chain reaction (PCR), and the presence of eight virulence genes were identified by multiplex PCR. A total of 125 E. coli isolates were subjected to analysis of phylogenetic background and virulence associated genes profiling. Phylogenetic analysis demonstrated that most of the E. coli isolated from chicken in this study belonged to group B1 (36.0%), group D (28.0%), group A (27.2%) and group B2 (8.8%). Multiplex PCR analysis demonstrated that 96 (78.6%) of the E. coli isolates harbored at least one virulence gene, while 29 (23.3%) did not contain any virulence genes tested. The most prevalent virulence genes identified were iss (51.2%), followed by iucD (36.0%), tsh (32.8%), vat (16.0%), astA (13.6%), irp2 (11. 2%), papC (9.6%) and the least is cva/cvi gene (0%). None of the isolates harbored more than four virulence genes. Each of phylogenetic groups presented with different combinations of virulence genes, with no specific combinations of virulence genes found to correlate with E. coli phylogroups. None of the E. coli isolates harbored more than four virulence genes, suggesting that E. coli isolates from chicken in this study appear to be derived from commensal strains and may relate to environmental predispose factors especially stress factors in the host to establish infection. Department of Veterinary Services Malaysia 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/56570/1/Phylogenetic%20grouping%20and%20virulence%20gene%20profiles%20of%20Escherichia%20coli%20isolated%20from%20chicken.pdf Roslee, Roseliza and Bejo, Siti Khairani and Zakaria, Zunita and M., Ramlan and Khoo, Evie (2017) Phylogenetic grouping and virulence gene profiles of Escherichia coli isolated from chicken. Malaysian Journal of Veterinary Research, 8 (1). pp. 65-74. ISSN 2180-3897 http://www.dvs.gov.my/index.php/pages/view/1663
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Colibacillosis is a disease caused by avian pathogenic E. coli (APEC) and is one of the principle cause of morbidity and mortality in poultry worldwide which is represented by a complex syndrome characterized by multiple organ lesions. This study was carried out to determine phylogenetic grouping and virulence associated genes contained by E. coli isolates which is related in causing disease in chicken. E. coli isolates obtained from clinical cases of Veterinary Research Institute were re-identified by conventional methods. Phylogenetic grouping of the isolates was determined by triplex polymerase chain reaction (PCR), and the presence of eight virulence genes were identified by multiplex PCR. A total of 125 E. coli isolates were subjected to analysis of phylogenetic background and virulence associated genes profiling. Phylogenetic analysis demonstrated that most of the E. coli isolated from chicken in this study belonged to group B1 (36.0%), group D (28.0%), group A (27.2%) and group B2 (8.8%). Multiplex PCR analysis demonstrated that 96 (78.6%) of the E. coli isolates harbored at least one virulence gene, while 29 (23.3%) did not contain any virulence genes tested. The most prevalent virulence genes identified were iss (51.2%), followed by iucD (36.0%), tsh (32.8%), vat (16.0%), astA (13.6%), irp2 (11. 2%), papC (9.6%) and the least is cva/cvi gene (0%). None of the isolates harbored more than four virulence genes. Each of phylogenetic groups presented with different combinations of virulence genes, with no specific combinations of virulence genes found to correlate with E. coli phylogroups. None of the E. coli isolates harbored more than four virulence genes, suggesting that E. coli isolates from chicken in this study appear to be derived from commensal strains and may relate to environmental predispose factors especially stress factors in the host to establish infection.
format Article
author Roslee, Roseliza
Bejo, Siti Khairani
Zakaria, Zunita
M., Ramlan
Khoo, Evie
spellingShingle Roslee, Roseliza
Bejo, Siti Khairani
Zakaria, Zunita
M., Ramlan
Khoo, Evie
Phylogenetic grouping and virulence gene profiles of Escherichia coli isolated from chicken
author_facet Roslee, Roseliza
Bejo, Siti Khairani
Zakaria, Zunita
M., Ramlan
Khoo, Evie
author_sort Roslee, Roseliza
title Phylogenetic grouping and virulence gene profiles of Escherichia coli isolated from chicken
title_short Phylogenetic grouping and virulence gene profiles of Escherichia coli isolated from chicken
title_full Phylogenetic grouping and virulence gene profiles of Escherichia coli isolated from chicken
title_fullStr Phylogenetic grouping and virulence gene profiles of Escherichia coli isolated from chicken
title_full_unstemmed Phylogenetic grouping and virulence gene profiles of Escherichia coli isolated from chicken
title_sort phylogenetic grouping and virulence gene profiles of escherichia coli isolated from chicken
publisher Department of Veterinary Services Malaysia
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/56570/1/Phylogenetic%20grouping%20and%20virulence%20gene%20profiles%20of%20Escherichia%20coli%20isolated%20from%20chicken.pdf
http://psasir.upm.edu.my/id/eprint/56570/
http://www.dvs.gov.my/index.php/pages/view/1663
_version_ 1643836230233751552
score 13.211869

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