Cytotoxic activity of coumarins from Micromelum minutum
The crude petroleum ether and chloroform extracts of Micromelum minutum (G. Frost.) Wright & Arn (Rutaceae) showed strong cytotoxic activity when tested against a T-lymphoblastic leukemia cell line. Further fractionation of the extracts resulted in the isolation of five new coumarins 3″,4″-dihyd...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Informa Healthcare
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/56238/1/Cytotoxic%20activity%20of%20coumarins%20from%20Micromelum%20minutum.pdf http://psasir.upm.edu.my/id/eprint/56238/ http://www.tandfonline.com/doi/abs/10.1080/13880200802436513 |
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| Summary: | The crude petroleum ether and chloroform extracts of Micromelum minutum (G. Frost.) Wright & Arn (Rutaceae) showed strong cytotoxic activity when tested against a T-lymphoblastic leukemia cell line. Further fractionation of the extracts resulted in the isolation of five new coumarins 3″,4″-dihydrocapnolactone, 2′,3′-epoxyisocapnolactone, 8-hydroxyisocapnolactone-29,39-diol, 8-hydroxy-3″,4″- dihydrocapnolactone-29,39-diol and 8,4″-dihydroxy-3″,4″- dihydrocapnolactone-29,39-diol, and two triterpenes. Some of these compounds were strongly active against T-lymphoblastic leukemia (CEM-SS), promyeolocytic leukemia (HL60), cervical cancer (HeLa) and liver cancer (HepG2) cell lines. 8-Hydroxyisocapnolactone-29,39-diol was found to be the most active with IC50 values of 2.9, 2.5, 6.9, and 5.9 μg/ml, respectively. This was followed by 2′,3′-epoxyisocapnolactone. When evaluated against the normal mouse fibroblast (3T3) cell line, 8-hydroxyisocapnolactone-29,39-diol was found to be inactive, hence it could serve as a valuable lead for further design and synthesis of more active analogues. |
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