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Prolific plant regeneration through organogenesis from scalps of Musa sp cv. Tanduk

A prolific plant regeneration system using scalps derived from shoot tips of Musaspp. cv. Tanduk was developed. Highly proliferating scalps, produced after four monthly subcultures of shoot tip explant on Murashige and Skoog (MS) medium supplemented with 100 mM BAP and 1.0 mM IAA, were placed on MS...

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Main Authors: Elhory, Suleiman Mohamed Ahmed, Abdul Aziz, Maheran, Abdul Rashid, Azmi, Yunus, Abdul Ghani
Format: Article
Language:English
Published: Academic Journals 2009
Online Access:http://psasir.upm.edu.my/id/eprint/5613/1/Prolific_plant_regeneration.pdf
http://psasir.upm.edu.my/id/eprint/5613/
http://www.academicjournals.org/journal/AJB/article-abstract/202BD3313899
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spelling my.upm.eprints.56132017-11-09T05:48:55Z http://psasir.upm.edu.my/id/eprint/5613/ Prolific plant regeneration through organogenesis from scalps of Musa sp cv. Tanduk Elhory, Suleiman Mohamed Ahmed Abdul Aziz, Maheran Abdul Rashid, Azmi Yunus, Abdul Ghani A prolific plant regeneration system using scalps derived from shoot tips of Musaspp. cv. Tanduk was developed. Highly proliferating scalps, produced after four monthly subcultures of shoot tip explant on Murashige and Skoog (MS) medium supplemented with 100 mM BAP and 1.0 mM IAA, were placed on MS basal medium supplemented with 1.0, 2.5 and 5.0 mM BAP. Rooting of shoots was assessed on hormone-free half strength and full strength MS media and on MS medium supplemented with 1.0, 5.0 and 10 mM IBA. Four types of potting media comprising of sand, peat, sand + top soil + goat dung (3:2:1 v/v) and top soil + sand (1:1 v/v) were evaluated during acclimatization of the plantlets. Prolific shoot regeneration from scalps was obtained on MS medium containing 2.5 mM BAP, at 9.61 and 40.6 shoots per explant after 4 and 8 weeks of culture, respectively. Meanwhile, the highest mean shoot height of 2.19 cm was attained on MS medium with 1.0 mM BAP after 8 weeks of culture. Full-strength MS medium supplemented with 5.0 mM IBA produced the highest mean number of roots per explant at 15.08, while the highest mean root length of 11.07 cm was obtained on hormone-free half strength MS medium at week 4 of culture. The highest plant survivability of 77.5% was achieved in potting medium consisting of top soil + sand + goat dung after 6 weeks of acclimatization. The plants were morphologically normal with vigorous stems and broad green leaves. Academic Journals 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/5613/1/Prolific_plant_regeneration.pdf Elhory, Suleiman Mohamed Ahmed and Abdul Aziz, Maheran and Abdul Rashid, Azmi and Yunus, Abdul Ghani (2009) Prolific plant regeneration through organogenesis from scalps of Musa sp cv. Tanduk. African Journal of Biotechnology, 8 (22). art. no. 202BD3313899. pp. 6208-6213. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/202BD3313899
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A prolific plant regeneration system using scalps derived from shoot tips of Musaspp. cv. Tanduk was developed. Highly proliferating scalps, produced after four monthly subcultures of shoot tip explant on Murashige and Skoog (MS) medium supplemented with 100 mM BAP and 1.0 mM IAA, were placed on MS basal medium supplemented with 1.0, 2.5 and 5.0 mM BAP. Rooting of shoots was assessed on hormone-free half strength and full strength MS media and on MS medium supplemented with 1.0, 5.0 and 10 mM IBA. Four types of potting media comprising of sand, peat, sand + top soil + goat dung (3:2:1 v/v) and top soil + sand (1:1 v/v) were evaluated during acclimatization of the plantlets. Prolific shoot regeneration from scalps was obtained on MS medium containing 2.5 mM BAP, at 9.61 and 40.6 shoots per explant after 4 and 8 weeks of culture, respectively. Meanwhile, the highest mean shoot height of 2.19 cm was attained on MS medium with 1.0 mM BAP after 8 weeks of culture. Full-strength MS medium supplemented with 5.0 mM IBA produced the highest mean number of roots per explant at 15.08, while the highest mean root length of 11.07 cm was obtained on hormone-free half strength MS medium at week 4 of culture. The highest plant survivability of 77.5% was achieved in potting medium consisting of top soil + sand + goat dung after 6 weeks of acclimatization. The plants were morphologically normal with vigorous stems and broad green leaves.
format Article
author Elhory, Suleiman Mohamed Ahmed
Abdul Aziz, Maheran
Abdul Rashid, Azmi
Yunus, Abdul Ghani
spellingShingle Elhory, Suleiman Mohamed Ahmed
Abdul Aziz, Maheran
Abdul Rashid, Azmi
Yunus, Abdul Ghani
Prolific plant regeneration through organogenesis from scalps of Musa sp cv. Tanduk
author_facet Elhory, Suleiman Mohamed Ahmed
Abdul Aziz, Maheran
Abdul Rashid, Azmi
Yunus, Abdul Ghani
author_sort Elhory, Suleiman Mohamed Ahmed
title Prolific plant regeneration through organogenesis from scalps of Musa sp cv. Tanduk
title_short Prolific plant regeneration through organogenesis from scalps of Musa sp cv. Tanduk
title_full Prolific plant regeneration through organogenesis from scalps of Musa sp cv. Tanduk
title_fullStr Prolific plant regeneration through organogenesis from scalps of Musa sp cv. Tanduk
title_full_unstemmed Prolific plant regeneration through organogenesis from scalps of Musa sp cv. Tanduk
title_sort prolific plant regeneration through organogenesis from scalps of musa sp cv. tanduk
publisher Academic Journals
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/5613/1/Prolific_plant_regeneration.pdf
http://psasir.upm.edu.my/id/eprint/5613/
http://www.academicjournals.org/journal/AJB/article-abstract/202BD3313899
_version_ 1643823241639231488
score 13.211869

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