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DNA barcoding and phylogenetic analysis of Malaysian groupers (Subfamily: Epinephelinae) using mitochondrial Cytochrome c oxidase I (COI) gene

The present study was carried out to examine the species identification and phylogenetic relationships of groupers in Malaysia using mitochondrial Cytochrome c Oxidase I (COI) gene, commonly known as barcoding gene. A total of 63 individuals comprising 10 species from three genera were collected fro...

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Main Authors: Abdul Aziz, Nurnadia Marshita, Esa, Yuzine, Arshad, Aziz
Format: Article
Language:English
Published: Triveni Enterprises, Lucknow 2016
Online Access:http://psasir.upm.edu.my/id/eprint/55551/1/DNA%20barcoding%20and%20phylogenetic%20analysis%20of%20Malaysian%20groupers%20.pdf
http://psasir.upm.edu.my/id/eprint/55551/
http://www.jeb.co.in/journal_issues/201607_jul16_spl/paper_12.pdf
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spelling my.upm.eprints.555512017-08-14T10:41:15Z http://psasir.upm.edu.my/id/eprint/55551/ DNA barcoding and phylogenetic analysis of Malaysian groupers (Subfamily: Epinephelinae) using mitochondrial Cytochrome c oxidase I (COI) gene Abdul Aziz, Nurnadia Marshita Esa, Yuzine Arshad, Aziz The present study was carried out to examine the species identification and phylogenetic relationships of groupers in Malaysia using mitochondrial Cytochrome c Oxidase I (COI) gene, commonly known as barcoding gene. A total of 63 individuals comprising 10 species from three genera were collected from the coastal areas of Johor, Kelantan, Pahang, Perak, Selangor and Terengganu. All the individuals were morphologically identified and molecular works involved polymerase chain reaction (PCR) and sequencing of COI barcoding fragment (655 base pairs). Results from the BLAST search showed that 55 sequences could be assigned to 10 grouper species with high percentage identity index (≥95% to 100%), discrepancies in their taxonomic identification based on the morphology and the COI barcoding results. The histogram of distances showed that there was a clear-cut barcode gap present in the sequences indicating a clear separation between intraspecific and interspecific distances. The pairwise genetic distances showed lowest pairwise distance between P. leopardus and P. maculatus (4.4%), while the highest pairwise distance was between E. bleekeri and P. maculatus (23.5%), supporting their morphological and habitat similarities and differences. Phylogenetic analysis (Neighbor-Joining) showed the presence of two major clades (1) genus Epinephelus vs (2) genus Plectropomus and Cephalopholis). In conclusion, the present study has managed to show the accuracy of DNA barcoding method for species identification, and utilization of COI gene for phylogenetic study among groupers. Triveni Enterprises, Lucknow 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55551/1/DNA%20barcoding%20and%20phylogenetic%20analysis%20of%20Malaysian%20groupers%20.pdf Abdul Aziz, Nurnadia Marshita and Esa, Yuzine and Arshad, Aziz (2016) DNA barcoding and phylogenetic analysis of Malaysian groupers (Subfamily: Epinephelinae) using mitochondrial Cytochrome c oxidase I (COI) gene. Journal of Environmental Biology, 37 (1). pp. 725-733. ISSN 0254-8704 http://www.jeb.co.in/journal_issues/201607_jul16_spl/paper_12.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The present study was carried out to examine the species identification and phylogenetic relationships of groupers in Malaysia using mitochondrial Cytochrome c Oxidase I (COI) gene, commonly known as barcoding gene. A total of 63 individuals comprising 10 species from three genera were collected from the coastal areas of Johor, Kelantan, Pahang, Perak, Selangor and Terengganu. All the individuals were morphologically identified and molecular works involved polymerase chain reaction (PCR) and sequencing of COI barcoding fragment (655 base pairs). Results from the BLAST search showed that 55 sequences could be assigned to 10 grouper species with high percentage identity index (≥95% to 100%), discrepancies in their taxonomic identification based on the morphology and the COI barcoding results. The histogram of distances showed that there was a clear-cut barcode gap present in the sequences indicating a clear separation between intraspecific and interspecific distances. The pairwise genetic distances showed lowest pairwise distance between P. leopardus and P. maculatus (4.4%), while the highest pairwise distance was between E. bleekeri and P. maculatus (23.5%), supporting their morphological and habitat similarities and differences. Phylogenetic analysis (Neighbor-Joining) showed the presence of two major clades (1) genus Epinephelus vs (2) genus Plectropomus and Cephalopholis). In conclusion, the present study has managed to show the accuracy of DNA barcoding method for species identification, and utilization of COI gene for phylogenetic study among groupers.
format Article
author Abdul Aziz, Nurnadia Marshita
Esa, Yuzine
Arshad, Aziz
spellingShingle Abdul Aziz, Nurnadia Marshita
Esa, Yuzine
Arshad, Aziz
DNA barcoding and phylogenetic analysis of Malaysian groupers (Subfamily: Epinephelinae) using mitochondrial Cytochrome c oxidase I (COI) gene
author_facet Abdul Aziz, Nurnadia Marshita
Esa, Yuzine
Arshad, Aziz
author_sort Abdul Aziz, Nurnadia Marshita
title DNA barcoding and phylogenetic analysis of Malaysian groupers (Subfamily: Epinephelinae) using mitochondrial Cytochrome c oxidase I (COI) gene
title_short DNA barcoding and phylogenetic analysis of Malaysian groupers (Subfamily: Epinephelinae) using mitochondrial Cytochrome c oxidase I (COI) gene
title_full DNA barcoding and phylogenetic analysis of Malaysian groupers (Subfamily: Epinephelinae) using mitochondrial Cytochrome c oxidase I (COI) gene
title_fullStr DNA barcoding and phylogenetic analysis of Malaysian groupers (Subfamily: Epinephelinae) using mitochondrial Cytochrome c oxidase I (COI) gene
title_full_unstemmed DNA barcoding and phylogenetic analysis of Malaysian groupers (Subfamily: Epinephelinae) using mitochondrial Cytochrome c oxidase I (COI) gene
title_sort dna barcoding and phylogenetic analysis of malaysian groupers (subfamily: epinephelinae) using mitochondrial cytochrome c oxidase i (coi) gene
publisher Triveni Enterprises, Lucknow
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/55551/1/DNA%20barcoding%20and%20phylogenetic%20analysis%20of%20Malaysian%20groupers%20.pdf
http://psasir.upm.edu.my/id/eprint/55551/
http://www.jeb.co.in/journal_issues/201607_jul16_spl/paper_12.pdf
_version_ 1643835928753471488
score 13.211869

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