Cover Image

Contributions of tropodithietic acid and biofilm formation to the probiotic activity of Phaeobacter inhibens

Background:The probiotic bacterium Phaeobacter inhibens strain S4Sm, isolated from the inner shell surface of a healthy oyster, secretes the antibiotic tropodithietic acid (TDA), is an excellent biofilm former, and increases oyster larvae survival when challenged with bacterial pathogens. In this st...

Full description

Saved in:
Bibliographic Details
Main Authors: Zhao, Wenjing, Dao, Christine, Karim, Murni, Gomez-Chiarri, Marta, Rowley, David, Nelson, David R.
Format: Article
Language:English
Published: BioMed Central 2016
Online Access:http://psasir.upm.edu.my/id/eprint/55380/1/Contributions%20of%20tropodithietic%20acid%20and.pdf
http://psasir.upm.edu.my/id/eprint/55380/
https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-015-0617-z
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.55380
record_format eprints
spelling my.upm.eprints.553802017-10-04T09:51:44Z http://psasir.upm.edu.my/id/eprint/55380/ Contributions of tropodithietic acid and biofilm formation to the probiotic activity of Phaeobacter inhibens Zhao, Wenjing Dao, Christine Karim, Murni Gomez-Chiarri, Marta Rowley, David Nelson, David R. Background:The probiotic bacterium Phaeobacter inhibens strain S4Sm, isolated from the inner shell surface of a healthy oyster, secretes the antibiotic tropodithietic acid (TDA), is an excellent biofilm former, and increases oyster larvae survival when challenged with bacterial pathogens. In this study, we investigated the specific roles of TDA secretion and biofilm formation in the probiotic activity of S4Sm. Results:Mutations in clpX (ATP-dependent ATPase) and exoP (an exopolysaccharide biosynthesis gene) were created by insertional mutagenesis using homologous recombination. Mutation of clpX resulted in the loss of TDA production, no decline in biofilm formation, and loss of the ability to inhibit the growth of Vibrio tubiashii and Vibrio anguillarum in co-colonization experiments. Mutation of exoP resulted in a ~60 % decline in biofilm formation, no decline in TDA production, and delayed inhibitory activity towards Vibrio pathogens in co-colonization experiments. Both clpX and exoP mutants exhibited reduced ability to protect oyster larvae from death when challenged by Vibrio tubiashii. Complementation of the clpX and exoP mutations restored the wild type phenotype. We also found that pre-colonization of surfaces by S4Sm was critical for this bacterium to inhibit pathogen colonization and growth. Conclusions:Our observations demonstrate that probiotic activity by P. inhibens S4Sm involves contributions from both biofilm formation and the production of the antibiotic TDA. Further, probiotic activity also requires colonization of surfaces by S4Sm prior to the introduction of the pathogen. BioMed Central 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55380/1/Contributions%20of%20tropodithietic%20acid%20and.pdf Zhao, Wenjing and Dao, Christine and Karim, Murni and Gomez-Chiarri, Marta and Rowley, David and Nelson, David R. (2016) Contributions of tropodithietic acid and biofilm formation to the probiotic activity of Phaeobacter inhibens. BMC Microbiology, 16 (1). pp. 1-17. ISSN 1471-2180 https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-015-0617-z 10.1186/s12866-015-0617-z
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background:The probiotic bacterium Phaeobacter inhibens strain S4Sm, isolated from the inner shell surface of a healthy oyster, secretes the antibiotic tropodithietic acid (TDA), is an excellent biofilm former, and increases oyster larvae survival when challenged with bacterial pathogens. In this study, we investigated the specific roles of TDA secretion and biofilm formation in the probiotic activity of S4Sm. Results:Mutations in clpX (ATP-dependent ATPase) and exoP (an exopolysaccharide biosynthesis gene) were created by insertional mutagenesis using homologous recombination. Mutation of clpX resulted in the loss of TDA production, no decline in biofilm formation, and loss of the ability to inhibit the growth of Vibrio tubiashii and Vibrio anguillarum in co-colonization experiments. Mutation of exoP resulted in a ~60 % decline in biofilm formation, no decline in TDA production, and delayed inhibitory activity towards Vibrio pathogens in co-colonization experiments. Both clpX and exoP mutants exhibited reduced ability to protect oyster larvae from death when challenged by Vibrio tubiashii. Complementation of the clpX and exoP mutations restored the wild type phenotype. We also found that pre-colonization of surfaces by S4Sm was critical for this bacterium to inhibit pathogen colonization and growth. Conclusions:Our observations demonstrate that probiotic activity by P. inhibens S4Sm involves contributions from both biofilm formation and the production of the antibiotic TDA. Further, probiotic activity also requires colonization of surfaces by S4Sm prior to the introduction of the pathogen.
format Article
author Zhao, Wenjing
Dao, Christine
Karim, Murni
Gomez-Chiarri, Marta
Rowley, David
Nelson, David R.
spellingShingle Zhao, Wenjing
Dao, Christine
Karim, Murni
Gomez-Chiarri, Marta
Rowley, David
Nelson, David R.
Contributions of tropodithietic acid and biofilm formation to the probiotic activity of Phaeobacter inhibens
author_facet Zhao, Wenjing
Dao, Christine
Karim, Murni
Gomez-Chiarri, Marta
Rowley, David
Nelson, David R.
author_sort Zhao, Wenjing
title Contributions of tropodithietic acid and biofilm formation to the probiotic activity of Phaeobacter inhibens
title_short Contributions of tropodithietic acid and biofilm formation to the probiotic activity of Phaeobacter inhibens
title_full Contributions of tropodithietic acid and biofilm formation to the probiotic activity of Phaeobacter inhibens
title_fullStr Contributions of tropodithietic acid and biofilm formation to the probiotic activity of Phaeobacter inhibens
title_full_unstemmed Contributions of tropodithietic acid and biofilm formation to the probiotic activity of Phaeobacter inhibens
title_sort contributions of tropodithietic acid and biofilm formation to the probiotic activity of phaeobacter inhibens
publisher BioMed Central
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/55380/1/Contributions%20of%20tropodithietic%20acid%20and.pdf
http://psasir.upm.edu.my/id/eprint/55380/
https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-015-0617-z
_version_ 1643835877642731520
score 13.211869

Similar Items