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Comparative chemical screening and genetic analysis reveal tentoxin as a new virulence factor in Cochliobolus miyabeanus, the causal agent of brown spot disease on rice

Brown spot disease, caused by Cochliobolus miyabeanus, is currently considered to be one of the most important yield reducers of rice (Oryza sativa L.). Despite its agricultural importance, little is known about the virulence mechanisms deployed by the fungus. Therefore, we set out to identify novel...

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Main Authors: De Bruyne, Lieselotte, Poucke, Christof Van, Di Mavungu, Diana Jose, Mohd Zainuddin, Nur Ain Izzati, Haecke, Lynn Van, Vleesschauwer, David De, Turgeon, B. Gillian, De Saeger, Sarah, Höfte, Monica
Format: Article
Language:English
Published: BSPP and John Wiley & Sons 2016
Online Access:http://psasir.upm.edu.my/id/eprint/55322/1/Comparative%20chemical%20screening%20and%20genetic%20analysis%20reveal%20tentoxin%20as%20a%20new%20virulence%20.pdf
http://psasir.upm.edu.my/id/eprint/55322/
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spelling my.upm.eprints.553222017-11-07T02:35:46Z http://psasir.upm.edu.my/id/eprint/55322/ Comparative chemical screening and genetic analysis reveal tentoxin as a new virulence factor in Cochliobolus miyabeanus, the causal agent of brown spot disease on rice De Bruyne, Lieselotte Poucke, Christof Van Di Mavungu, Diana Jose Mohd Zainuddin, Nur Ain Izzati Haecke, Lynn Van Vleesschauwer, David De Turgeon, B. Gillian De Saeger, Sarah Höfte, Monica Brown spot disease, caused by Cochliobolus miyabeanus, is currently considered to be one of the most important yield reducers of rice (Oryza sativa L.). Despite its agricultural importance, little is known about the virulence mechanisms deployed by the fungus. Therefore, we set out to identify novel virulence factors with a role in disease development. This article reports, for the first time, the production of tentoxin by C. miyabeanus as a virulence factor during brown spot disease and the identification of the non-ribosomal protein synthetase (NRPS) CmNps3, responsible for tentoxin biosynthesis. We compared the chemical compounds produced by C. miyabeanus strains differing in virulence ability using ultra-high-performance liquid chromatography (UHPLC) coupled to high-resolution Orbitrap mass spectrometry (HRMS). The production of tentoxin by a highly virulent strain was revealed by principal component analysis of the detected ions and confirmed by UHPLC coupled to tandem-quadrupole mass spectrometry (MS/MS). The corresponding NRPS was identified by in silico genome analysis and confirmed by gene deletion. Infection tests with wild-type and Cmnps3 mutants showed that tentoxin acts as a virulence factor and is correlated with chlorosis development during the second phase of infection. Although rice has previously been classified as a tentoxin-insensitive plant species, our data demonstrate that tentoxin production by C. miyabeanus affects symptom development. BSPP and John Wiley & Sons 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55322/1/Comparative%20chemical%20screening%20and%20genetic%20analysis%20reveal%20tentoxin%20as%20a%20new%20virulence%20.pdf De Bruyne, Lieselotte and Poucke, Christof Van and Di Mavungu, Diana Jose and Mohd Zainuddin, Nur Ain Izzati and Haecke, Lynn Van and Vleesschauwer, David De and Turgeon, B. Gillian and De Saeger, Sarah and Höfte, Monica (2016) Comparative chemical screening and genetic analysis reveal tentoxin as a new virulence factor in Cochliobolus miyabeanus, the causal agent of brown spot disease on rice. Molecular Plant Pathology, 17 (6). pp. 805-817. ISSN 1464-672; ESSN: 1364-3703 10.1111/mpp.12329
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Brown spot disease, caused by Cochliobolus miyabeanus, is currently considered to be one of the most important yield reducers of rice (Oryza sativa L.). Despite its agricultural importance, little is known about the virulence mechanisms deployed by the fungus. Therefore, we set out to identify novel virulence factors with a role in disease development. This article reports, for the first time, the production of tentoxin by C. miyabeanus as a virulence factor during brown spot disease and the identification of the non-ribosomal protein synthetase (NRPS) CmNps3, responsible for tentoxin biosynthesis. We compared the chemical compounds produced by C. miyabeanus strains differing in virulence ability using ultra-high-performance liquid chromatography (UHPLC) coupled to high-resolution Orbitrap mass spectrometry (HRMS). The production of tentoxin by a highly virulent strain was revealed by principal component analysis of the detected ions and confirmed by UHPLC coupled to tandem-quadrupole mass spectrometry (MS/MS). The corresponding NRPS was identified by in silico genome analysis and confirmed by gene deletion. Infection tests with wild-type and Cmnps3 mutants showed that tentoxin acts as a virulence factor and is correlated with chlorosis development during the second phase of infection. Although rice has previously been classified as a tentoxin-insensitive plant species, our data demonstrate that tentoxin production by C. miyabeanus affects symptom development.
format Article
author De Bruyne, Lieselotte
Poucke, Christof Van
Di Mavungu, Diana Jose
Mohd Zainuddin, Nur Ain Izzati
Haecke, Lynn Van
Vleesschauwer, David De
Turgeon, B. Gillian
De Saeger, Sarah
Höfte, Monica
spellingShingle De Bruyne, Lieselotte
Poucke, Christof Van
Di Mavungu, Diana Jose
Mohd Zainuddin, Nur Ain Izzati
Haecke, Lynn Van
Vleesschauwer, David De
Turgeon, B. Gillian
De Saeger, Sarah
Höfte, Monica
Comparative chemical screening and genetic analysis reveal tentoxin as a new virulence factor in Cochliobolus miyabeanus, the causal agent of brown spot disease on rice
author_facet De Bruyne, Lieselotte
Poucke, Christof Van
Di Mavungu, Diana Jose
Mohd Zainuddin, Nur Ain Izzati
Haecke, Lynn Van
Vleesschauwer, David De
Turgeon, B. Gillian
De Saeger, Sarah
Höfte, Monica
author_sort De Bruyne, Lieselotte
title Comparative chemical screening and genetic analysis reveal tentoxin as a new virulence factor in Cochliobolus miyabeanus, the causal agent of brown spot disease on rice
title_short Comparative chemical screening and genetic analysis reveal tentoxin as a new virulence factor in Cochliobolus miyabeanus, the causal agent of brown spot disease on rice
title_full Comparative chemical screening and genetic analysis reveal tentoxin as a new virulence factor in Cochliobolus miyabeanus, the causal agent of brown spot disease on rice
title_fullStr Comparative chemical screening and genetic analysis reveal tentoxin as a new virulence factor in Cochliobolus miyabeanus, the causal agent of brown spot disease on rice
title_full_unstemmed Comparative chemical screening and genetic analysis reveal tentoxin as a new virulence factor in Cochliobolus miyabeanus, the causal agent of brown spot disease on rice
title_sort comparative chemical screening and genetic analysis reveal tentoxin as a new virulence factor in cochliobolus miyabeanus, the causal agent of brown spot disease on rice
publisher BSPP and John Wiley & Sons
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/55322/1/Comparative%20chemical%20screening%20and%20genetic%20analysis%20reveal%20tentoxin%20as%20a%20new%20virulence%20.pdf
http://psasir.upm.edu.my/id/eprint/55322/
_version_ 1643835860466008064
score 13.211869

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