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Characterization of partitioning behaviors of immunoglobulin G in polymer-salt aqueous two-phase systems

The partitioning behavior of immunoglobulin G (IgG) in the aqueous two-phase system (ATPS) composed of poly(ethylene glycol) (PEG) and phosphate was studied. The parameters of ATPS exhibiting the pronounced effects on the partitioning behavior of IgG include phase composition, PEG molecular weight,...

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Main Authors: Yin, Hui Chow, Yee, Jiun Yap, Pau, Loke Show, Joon, Ching Juan, Anuar, Mohd Shamsul, Ng, Eng-Poh, Ooi, Chien-Wei, Tau, Chuan Ling
Format: Article
Language:English
Published: The Society for Biotechnology, Japan 2016
Online Access:http://psasir.upm.edu.my/id/eprint/55270/1/Characterization%20of%20partitioning%20behaviors%20of%20immunoglobulin%20G%20in%20polymer-salt%20.pdf
http://psasir.upm.edu.my/id/eprint/55270/
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spelling my.upm.eprints.552702018-08-15T01:57:16Z http://psasir.upm.edu.my/id/eprint/55270/ Characterization of partitioning behaviors of immunoglobulin G in polymer-salt aqueous two-phase systems Yin, Hui Chow Yee, Jiun Yap Pau, Loke Show Joon, Ching Juan Anuar, Mohd Shamsul Ng, Eng-Poh Ooi, Chien-Wei Tau, Chuan Ling The partitioning behavior of immunoglobulin G (IgG) in the aqueous two-phase system (ATPS) composed of poly(ethylene glycol) (PEG) and phosphate was studied. The parameters of ATPS exhibiting the pronounced effects on the partitioning behavior of IgG include phase composition, PEG molecular weight, and the addition of sodium chloride (NaCl). The accumulation of IgG at the interface of the ATPS increased drastically as the tie-line length (TLL) was increased. This trend was correlated with a linear relationship relating the natural logarithm of interfacial partition coefficient (ln G) to the difference of PEG concentration between the top phase and the bottom phase (Δ[PEG]), and a good fit was obtained. An attempt was made to correlate the natural logarithm of partition coefficient (ln K) to the presence of NaCl with the proposed linear relationship, ln K = α″ ln [Cl-] + β″. The proposed relationship, which serves as a better description of the underlying mechanics of the protein partitioning behavior in the polymer-salt ATPS, provides a good fit (r2 > 0.95) for the data of IgG partitioning. An optimum recovery of 99.97% was achieved in an ATPS (pH 7.5) composed of 14.0% (w/w) PEG 1450, 12.5% (w/w) phosphate and 5.0% (w/w) NaCl. The Society for Biotechnology, Japan 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/55270/1/Characterization%20of%20partitioning%20behaviors%20of%20immunoglobulin%20G%20in%20polymer-salt%20.pdf Yin, Hui Chow and Yee, Jiun Yap and Pau, Loke Show and Joon, Ching Juan and Anuar, Mohd Shamsul and Ng, Eng-Poh and Ooi, Chien-Wei and Tau, Chuan Ling (2016) Characterization of partitioning behaviors of immunoglobulin G in polymer-salt aqueous two-phase systems. Journal of Bioscience and Bioengineering, 122 (5). pp. 613-619. ISSN 1389-1723; ESSN: 1347-4421 10.1016/j.jbiosc.2016.04.008
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The partitioning behavior of immunoglobulin G (IgG) in the aqueous two-phase system (ATPS) composed of poly(ethylene glycol) (PEG) and phosphate was studied. The parameters of ATPS exhibiting the pronounced effects on the partitioning behavior of IgG include phase composition, PEG molecular weight, and the addition of sodium chloride (NaCl). The accumulation of IgG at the interface of the ATPS increased drastically as the tie-line length (TLL) was increased. This trend was correlated with a linear relationship relating the natural logarithm of interfacial partition coefficient (ln G) to the difference of PEG concentration between the top phase and the bottom phase (Δ[PEG]), and a good fit was obtained. An attempt was made to correlate the natural logarithm of partition coefficient (ln K) to the presence of NaCl with the proposed linear relationship, ln K = α″ ln [Cl-] + β″. The proposed relationship, which serves as a better description of the underlying mechanics of the protein partitioning behavior in the polymer-salt ATPS, provides a good fit (r2 > 0.95) for the data of IgG partitioning. An optimum recovery of 99.97% was achieved in an ATPS (pH 7.5) composed of 14.0% (w/w) PEG 1450, 12.5% (w/w) phosphate and 5.0% (w/w) NaCl.
format Article
author Yin, Hui Chow
Yee, Jiun Yap
Pau, Loke Show
Joon, Ching Juan
Anuar, Mohd Shamsul
Ng, Eng-Poh
Ooi, Chien-Wei
Tau, Chuan Ling
spellingShingle Yin, Hui Chow
Yee, Jiun Yap
Pau, Loke Show
Joon, Ching Juan
Anuar, Mohd Shamsul
Ng, Eng-Poh
Ooi, Chien-Wei
Tau, Chuan Ling
Characterization of partitioning behaviors of immunoglobulin G in polymer-salt aqueous two-phase systems
author_facet Yin, Hui Chow
Yee, Jiun Yap
Pau, Loke Show
Joon, Ching Juan
Anuar, Mohd Shamsul
Ng, Eng-Poh
Ooi, Chien-Wei
Tau, Chuan Ling
author_sort Yin, Hui Chow
title Characterization of partitioning behaviors of immunoglobulin G in polymer-salt aqueous two-phase systems
title_short Characterization of partitioning behaviors of immunoglobulin G in polymer-salt aqueous two-phase systems
title_full Characterization of partitioning behaviors of immunoglobulin G in polymer-salt aqueous two-phase systems
title_fullStr Characterization of partitioning behaviors of immunoglobulin G in polymer-salt aqueous two-phase systems
title_full_unstemmed Characterization of partitioning behaviors of immunoglobulin G in polymer-salt aqueous two-phase systems
title_sort characterization of partitioning behaviors of immunoglobulin g in polymer-salt aqueous two-phase systems
publisher The Society for Biotechnology, Japan
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/55270/1/Characterization%20of%20partitioning%20behaviors%20of%20immunoglobulin%20G%20in%20polymer-salt%20.pdf
http://psasir.upm.edu.my/id/eprint/55270/
_version_ 1643835846393069568
score 13.211869

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