Characterization of novel type I ribosome-inactivating proteins isolated from oil palm (Elaeis guineensis) inoculated with Ganoderma boninense, the causal agent of basal stem rot
Type I RIPs have not been identified in oil palm. EgRIP-1a and EgRIP-1b transcripts were partially isolated from roots and basal stems of oil palm seedlings with high similarity to type I RIPs. Expression levels were altered during plant-pathogen interaction and their proteins were 28–30 kD with an...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55269/1/Characterization%20of%20novel%20type%20I%20ribosome-inactivating%20proteins%20isolated%20from%20oil%20palm%20.pdf http://psasir.upm.edu.my/id/eprint/55269/ |
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| Summary: | Type I RIPs have not been identified in oil palm. EgRIP-1a and EgRIP-1b transcripts were partially isolated from roots and basal stems of oil palm seedlings with high similarity to type I RIPs. Expression levels were altered during plant-pathogen interaction and their proteins were 28–30 kD with an estimated pI value of 10.0. Depurination of yeast 26S rRNA by EgRIP proteins demonstrated specific ribosome-inactivating activity. EgRIP proteins showed inhibition on G. boninense mycelial growth with 44.1% at 5 DAI. It was concluded that the novel EgRIPs are type I and demonstrated to have antifungal effect against G. boninense. |
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