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Channa striatus cream down-regulates tumour necrosis factor (TNF)-alpha gene expression and alleviates chronic-like dermatitis in mouse model

Ethnopharmacological relevance: Haruan, Channa striatus, is a freshwater fish which has been well-known locally to accelerate wound healing during post-operative and post-partum periods. The fish extract also has potent anti-inflammatory and analgesic properties. Aim of the study: To assess to...

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Main Authors: Mohamad Isa, Irma Izani, Abu Bakar, Suhaili, Md Tohid, Siti Farah, Mat Jais, Abdul Manan
Format: Article
Language:English
Published: Elsevier Ireland 2016
Online Access:http://psasir.upm.edu.my/id/eprint/55246/1/Channa%20striatus%20cream%20down-regulates%20tumour%20necrosis%20factor%20%28TNF%29-alpha%20gene%20expression%20and%20alleviates%20chronic-like%20dermatitis%20in%20mouse%20model.pdf
http://psasir.upm.edu.my/id/eprint/55246/
http://www.elsevier.com/locate/issn/03788741
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spelling my.upm.eprints.552462018-01-23T05:12:21Z http://psasir.upm.edu.my/id/eprint/55246/ Channa striatus cream down-regulates tumour necrosis factor (TNF)-alpha gene expression and alleviates chronic-like dermatitis in mouse model Mohamad Isa, Irma Izani Abu Bakar, Suhaili Md Tohid, Siti Farah Mat Jais, Abdul Manan Ethnopharmacological relevance: Haruan, Channa striatus, is a freshwater fish which has been well-known locally to accelerate wound healing during post-operative and post-partum periods. The fish extract also has potent anti-inflammatory and analgesic properties. Aim of the study: To assess topical anti-inflammatory effect of Haruan cream on 12-0-tetradecanoylphorbol-13-acetate (TPA)-induced chronic-like dermatitis in mice. Materials and methods: Male ICR mice were randomized into six groups of five mice each: acetone (vehicle), TPA alone (negative control), three Haruan treatment groups (Haruan 1%, Haruan 5% and Haruan 10%) and hydrocortisone 1% (positive control). Briefly, both surfaces of mouse ears were applied with TPA (2.5μg/20μl acetone) for five times on alternate days and with Haruan or hydrocortisone 1% cream for the last three days. Mouse ear thickness was measured 24h after final treatment with the cream and the ears were harvested for further histological analysis and gene expression studies of TNF-α by real-time reverse transcriptase-polymerase chain reaction (RT-qPCR). Results: Topical application of Haruan cream had reduced the mouse ear thickness 18.1-28%) with comparable effect to the positive control. In addition, histopathological comparison had shown evident reduction in various parameters of cutaneous inflammation including dermal oedema, inflammatory cells infiltration and proliferation of epidermal keratinocytes. Furthermore, TPA application had resulted in the up-regulation of TNF-α gene expression by 353-fold, which was subsequently down-regulated by the Haruan cream (34- to 112-fold). Conclusion: Haruan is an effective topical anti-inflammatory agent in this mouse model of chronic-like dermatitis, thus suggesting its potential as a non-steroidal treatment option for chronic inflammatory dermatoses. Elsevier Ireland 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55246/1/Channa%20striatus%20cream%20down-regulates%20tumour%20necrosis%20factor%20%28TNF%29-alpha%20gene%20expression%20and%20alleviates%20chronic-like%20dermatitis%20in%20mouse%20model.pdf Mohamad Isa, Irma Izani and Abu Bakar, Suhaili and Md Tohid, Siti Farah and Mat Jais, Abdul Manan (2016) Channa striatus cream down-regulates tumour necrosis factor (TNF)-alpha gene expression and alleviates chronic-like dermatitis in mouse model. Journal of Ethnopharmacology, 194. pp. 469-474. ISSN 0378-8741; ESSN: 1872-7573 http://www.elsevier.com/locate/issn/03788741 10.1016/j.jep.2016.10.033
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Ethnopharmacological relevance: Haruan, Channa striatus, is a freshwater fish which has been well-known locally to accelerate wound healing during post-operative and post-partum periods. The fish extract also has potent anti-inflammatory and analgesic properties. Aim of the study: To assess topical anti-inflammatory effect of Haruan cream on 12-0-tetradecanoylphorbol-13-acetate (TPA)-induced chronic-like dermatitis in mice. Materials and methods: Male ICR mice were randomized into six groups of five mice each: acetone (vehicle), TPA alone (negative control), three Haruan treatment groups (Haruan 1%, Haruan 5% and Haruan 10%) and hydrocortisone 1% (positive control). Briefly, both surfaces of mouse ears were applied with TPA (2.5μg/20μl acetone) for five times on alternate days and with Haruan or hydrocortisone 1% cream for the last three days. Mouse ear thickness was measured 24h after final treatment with the cream and the ears were harvested for further histological analysis and gene expression studies of TNF-α by real-time reverse transcriptase-polymerase chain reaction (RT-qPCR). Results: Topical application of Haruan cream had reduced the mouse ear thickness 18.1-28%) with comparable effect to the positive control. In addition, histopathological comparison had shown evident reduction in various parameters of cutaneous inflammation including dermal oedema, inflammatory cells infiltration and proliferation of epidermal keratinocytes. Furthermore, TPA application had resulted in the up-regulation of TNF-α gene expression by 353-fold, which was subsequently down-regulated by the Haruan cream (34- to 112-fold). Conclusion: Haruan is an effective topical anti-inflammatory agent in this mouse model of chronic-like dermatitis, thus suggesting its potential as a non-steroidal treatment option for chronic inflammatory dermatoses.
format Article
author Mohamad Isa, Irma Izani
Abu Bakar, Suhaili
Md Tohid, Siti Farah
Mat Jais, Abdul Manan
spellingShingle Mohamad Isa, Irma Izani
Abu Bakar, Suhaili
Md Tohid, Siti Farah
Mat Jais, Abdul Manan
Channa striatus cream down-regulates tumour necrosis factor (TNF)-alpha gene expression and alleviates chronic-like dermatitis in mouse model
author_facet Mohamad Isa, Irma Izani
Abu Bakar, Suhaili
Md Tohid, Siti Farah
Mat Jais, Abdul Manan
author_sort Mohamad Isa, Irma Izani
title Channa striatus cream down-regulates tumour necrosis factor (TNF)-alpha gene expression and alleviates chronic-like dermatitis in mouse model
title_short Channa striatus cream down-regulates tumour necrosis factor (TNF)-alpha gene expression and alleviates chronic-like dermatitis in mouse model
title_full Channa striatus cream down-regulates tumour necrosis factor (TNF)-alpha gene expression and alleviates chronic-like dermatitis in mouse model
title_fullStr Channa striatus cream down-regulates tumour necrosis factor (TNF)-alpha gene expression and alleviates chronic-like dermatitis in mouse model
title_full_unstemmed Channa striatus cream down-regulates tumour necrosis factor (TNF)-alpha gene expression and alleviates chronic-like dermatitis in mouse model
title_sort channa striatus cream down-regulates tumour necrosis factor (tnf)-alpha gene expression and alleviates chronic-like dermatitis in mouse model
publisher Elsevier Ireland
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/55246/1/Channa%20striatus%20cream%20down-regulates%20tumour%20necrosis%20factor%20%28TNF%29-alpha%20gene%20expression%20and%20alleviates%20chronic-like%20dermatitis%20in%20mouse%20model.pdf
http://psasir.upm.edu.my/id/eprint/55246/
http://www.elsevier.com/locate/issn/03788741
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score 13.211869

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