Blood profiles and histopathological changes of liver and kidney tissues from male sprague dawley rats treated with ethanol extracts of Clinacanthus nutans leaf
Background: Clinacanthus nutans (C. nutans) or commonly known as ‘Sabah snake grass’ or ‘Belalai Gajah’ is a widely known herb used to treat Herpes simplex virus (HPV), skin rashes and snake bite. Objective: This study aim is to evaluate the toxicity of ethanol extract of C. nutans leaf extract o...
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Omics Publishing Group
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55217/1/Blood%20profiles%20and%20histopathological%20changes%20of%20liver%20and%20kidney%20tissues%20from%20male%20sprague%20dawley%20rats%20treated%20with%20ethanol%20extracts%20of%20Clinacanthus%20nutans%20leaf.pdf http://psasir.upm.edu.my/id/eprint/55217/ http://www.omicsonline.org/clinical-toxicology.php |
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my.upm.eprints.552172017-11-29T08:08:10Z http://psasir.upm.edu.my/id/eprint/55217/ Blood profiles and histopathological changes of liver and kidney tissues from male sprague dawley rats treated with ethanol extracts of Clinacanthus nutans leaf Sajjarattul Nurul Nadia Asyura, Hamzah, Hazilawati Mohamad Shaari, Rosly Sithambaram, Shanmugavelu Mohamed Mustapha, Noordin Background: Clinacanthus nutans (C. nutans) or commonly known as ‘Sabah snake grass’ or ‘Belalai Gajah’ is a widely known herb used to treat Herpes simplex virus (HPV), skin rashes and snake bite. Objective: This study aim is to evaluate the toxicity of ethanol extract of C. nutans leaf extract on 90-day sub chronic toxicity study in male Sprague Dawley rats. Method: A total of 40, 6-week male Sprague Dawley rats were divided into 5 groups (n=8) namely control, vehicle (10% DMSO), and 3 treatment groups which received a daily oral dose of C. nutans leaf extract at 75 (low dose), 125 (medium dose), and 250 (high dose) mg/kg for 90 days via oral gavage. Blood sample were collected at the end of the experiment for evaluation of haematology and serum biochemistry. Selected organs including liver and kidneys were collected for histopathological examination. The toxicity were evaluated by observing and evaluating the changes of body weight, haematology and serum biochemistry parameters and histopathology changes of liver and kidney tissues. Results: There was no mortality sign of sub chronic toxicity observed during the observation period. Body weight, haematology parameters and organ relative weight showed no significant difference in control and treatment groups meanwhile in serum biochemistry parameters, observed a significant difference (P<0.05) in level of LDH and creatinine kinase in high dose group showed significant lower(P<0.05) compared to control. Nevertheless the levels of LDH and creatinine kinase were still in normal range. Significant abnormal histopathological changes (P<0.05) such as centrilobular sinusoid dilatation/centrilobular necrosis, hydropic degeneration/cytoplasmic vacuolation and inflammation were observed in liver tissues in medium and high dose groups. Kidney tissue showed a significant abnormal histopathology changes (P<0.05) such as granular cast and cellular cast were observed in medium and high dose group. Conclusion: It is concluded that 125-250 mg/kg ethanol extract of C. nutans leaves induce hepatotoxicity and renal toxicity. Omics Publishing Group 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55217/1/Blood%20profiles%20and%20histopathological%20changes%20of%20liver%20and%20kidney%20tissues%20from%20male%20sprague%20dawley%20rats%20treated%20with%20ethanol%20extracts%20of%20Clinacanthus%20nutans%20leaf.pdf Sajjarattul Nurul Nadia Asyura, and Hamzah, Hazilawati and Mohamad Shaari, Rosly and Sithambaram, Shanmugavelu and Mohamed Mustapha, Noordin (2016) Blood profiles and histopathological changes of liver and kidney tissues from male sprague dawley rats treated with ethanol extracts of Clinacanthus nutans leaf. Journal of Clinical Toxicology, 6 (6). pp. 1-10. ISSN ESSN: 2161-0495 http://www.omicsonline.org/clinical-toxicology.php 10.4172/2161-0495.1000329 |
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Background: Clinacanthus nutans (C. nutans) or commonly known as ‘Sabah snake grass’ or ‘Belalai Gajah’ is a widely known herb used to treat Herpes simplex virus (HPV), skin rashes and snake bite.
Objective: This study aim is to evaluate the toxicity of ethanol extract of C. nutans leaf extract on 90-day sub chronic toxicity study in male Sprague Dawley rats.
Method: A total of 40, 6-week male Sprague Dawley rats were divided into 5 groups (n=8) namely control, vehicle (10% DMSO), and 3 treatment groups which received a daily oral dose of C. nutans leaf extract at 75 (low dose), 125 (medium dose), and 250 (high dose) mg/kg for 90 days via oral gavage. Blood sample were collected at the end of the experiment for evaluation of haematology and serum biochemistry. Selected organs including liver and kidneys were collected for histopathological examination. The toxicity were evaluated by observing and evaluating the changes of body weight, haematology and serum biochemistry parameters and histopathology changes of liver and kidney tissues.
Results: There was no mortality sign of sub chronic toxicity observed during the observation period. Body weight, haematology parameters and organ relative weight showed no significant difference in control and treatment groups meanwhile in serum biochemistry parameters, observed a significant difference (P<0.05) in level of LDH and creatinine kinase in high dose group showed significant lower(P<0.05) compared to control. Nevertheless the levels of LDH and creatinine kinase were still in normal range. Significant abnormal histopathological changes (P<0.05) such as centrilobular sinusoid dilatation/centrilobular necrosis, hydropic degeneration/cytoplasmic vacuolation and inflammation were observed in liver tissues in medium and high dose groups. Kidney tissue showed a significant abnormal histopathology changes (P<0.05) such as granular cast and cellular cast were observed in medium and high dose group.
Conclusion: It is concluded that 125-250 mg/kg ethanol extract of C. nutans leaves induce hepatotoxicity and renal toxicity. |
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Article |
| author |
Sajjarattul Nurul Nadia Asyura, Hamzah, Hazilawati Mohamad Shaari, Rosly Sithambaram, Shanmugavelu Mohamed Mustapha, Noordin |
| spellingShingle |
Sajjarattul Nurul Nadia Asyura, Hamzah, Hazilawati Mohamad Shaari, Rosly Sithambaram, Shanmugavelu Mohamed Mustapha, Noordin Blood profiles and histopathological changes of liver and kidney tissues from male sprague dawley rats treated with ethanol extracts of Clinacanthus nutans leaf |
| author_facet |
Sajjarattul Nurul Nadia Asyura, Hamzah, Hazilawati Mohamad Shaari, Rosly Sithambaram, Shanmugavelu Mohamed Mustapha, Noordin |
| author_sort |
Sajjarattul Nurul Nadia Asyura, |
| title |
Blood profiles and histopathological changes of liver and kidney tissues from male sprague dawley rats treated with ethanol extracts of Clinacanthus nutans leaf |
| title_short |
Blood profiles and histopathological changes of liver and kidney tissues from male sprague dawley rats treated with ethanol extracts of Clinacanthus nutans leaf |
| title_full |
Blood profiles and histopathological changes of liver and kidney tissues from male sprague dawley rats treated with ethanol extracts of Clinacanthus nutans leaf |
| title_fullStr |
Blood profiles and histopathological changes of liver and kidney tissues from male sprague dawley rats treated with ethanol extracts of Clinacanthus nutans leaf |
| title_full_unstemmed |
Blood profiles and histopathological changes of liver and kidney tissues from male sprague dawley rats treated with ethanol extracts of Clinacanthus nutans leaf |
| title_sort |
blood profiles and histopathological changes of liver and kidney tissues from male sprague dawley rats treated with ethanol extracts of clinacanthus nutans leaf |
| publisher |
Omics Publishing Group |
| publishDate |
2016 |
| url |
http://psasir.upm.edu.my/id/eprint/55217/1/Blood%20profiles%20and%20histopathological%20changes%20of%20liver%20and%20kidney%20tissues%20from%20male%20sprague%20dawley%20rats%20treated%20with%20ethanol%20extracts%20of%20Clinacanthus%20nutans%20leaf.pdf http://psasir.upm.edu.my/id/eprint/55217/ http://www.omicsonline.org/clinical-toxicology.php |
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13.211869 |