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Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae

A Streptomyces isolate having antifungal activity against Pyricularia oryzae, the causal agent of rice blast disease, was isolated from soil collected in rice fields of Tanjung Karang Selangor, peninsula Malaysia.The aim of the study was to determine the antifungal activity of Streptomyces sp. isola...

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Main Authors: Awla, Hayman Kakakhan, Kadir, Jugah, Othman, Radziah, Rashid, Tavga Sulaiman, Wong, Mui Yun
Format: Article
Language:English
Published: Scientific Research Publishing 2016
Online Access:http://psasir.upm.edu.my/id/eprint/55200/1/Bioactive%20compounds%20produced%20by%20Streptomyces%20sp.%20isolate%20UPMRS4%20and%20antifungal%20activity%20against%20Pyricularia%20oryzae.pdf
http://psasir.upm.edu.my/id/eprint/55200/
http://www.scirp.org/journal/ajps
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spelling my.upm.eprints.552002018-01-22T10:16:16Z http://psasir.upm.edu.my/id/eprint/55200/ Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae Awla, Hayman Kakakhan Kadir, Jugah Othman, Radziah Rashid, Tavga Sulaiman Wong, Mui Yun A Streptomyces isolate having antifungal activity against Pyricularia oryzae, the causal agent of rice blast disease, was isolated from soil collected in rice fields of Tanjung Karang Selangor, peninsula Malaysia.The aim of the study was to determine the antifungal activity of Streptomyces sp. isolate UPMRS4 extracts against P. oryzae and to identify bioactive antifungal compounds produced by UPMRS4. Various solvents were used for extraction of antifungal compounds and well diffusion method was used to determine the antifungal activity of the extracts. The ethyl acetate extract demonstrated the highest activity against mycelial growth of P. oryzae, with an effective inhibitory concentration (EIC) of 1.562 μg/ml significantly higher compared to that of chloroform, diethyl ether, methanol, acetone, ethanol and water. Based on GC-MS and LC-MS/MSanalyses,compounds with antifungal activity were detected such as (Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl); Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(phenylmethyl); ergotamine; amicomacin; fungichromin; rapamycin and N-Acetyl-D, L-phenylalanine. These compounds had good general antifungal activity and might have potential future agricultural applications. Scientific Research Publishing 2016-05 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55200/1/Bioactive%20compounds%20produced%20by%20Streptomyces%20sp.%20isolate%20UPMRS4%20and%20antifungal%20activity%20against%20Pyricularia%20oryzae.pdf Awla, Hayman Kakakhan and Kadir, Jugah and Othman, Radziah and Rashid, Tavga Sulaiman and Wong, Mui Yun (2016) Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae. American Journal of Plant Sciences, 7 (7). art. no. 66540. pp. 1077-1085. ISSN 2158-2742; ESSN: 2158-2750 http://www.scirp.org/journal/ajps 10.4236/ajps.2016.77103
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A Streptomyces isolate having antifungal activity against Pyricularia oryzae, the causal agent of rice blast disease, was isolated from soil collected in rice fields of Tanjung Karang Selangor, peninsula Malaysia.The aim of the study was to determine the antifungal activity of Streptomyces sp. isolate UPMRS4 extracts against P. oryzae and to identify bioactive antifungal compounds produced by UPMRS4. Various solvents were used for extraction of antifungal compounds and well diffusion method was used to determine the antifungal activity of the extracts. The ethyl acetate extract demonstrated the highest activity against mycelial growth of P. oryzae, with an effective inhibitory concentration (EIC) of 1.562 μg/ml significantly higher compared to that of chloroform, diethyl ether, methanol, acetone, ethanol and water. Based on GC-MS and LC-MS/MSanalyses,compounds with antifungal activity were detected such as (Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl); Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(phenylmethyl); ergotamine; amicomacin; fungichromin; rapamycin and N-Acetyl-D, L-phenylalanine. These compounds had good general antifungal activity and might have potential future agricultural applications.
format Article
author Awla, Hayman Kakakhan
Kadir, Jugah
Othman, Radziah
Rashid, Tavga Sulaiman
Wong, Mui Yun
spellingShingle Awla, Hayman Kakakhan
Kadir, Jugah
Othman, Radziah
Rashid, Tavga Sulaiman
Wong, Mui Yun
Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae
author_facet Awla, Hayman Kakakhan
Kadir, Jugah
Othman, Radziah
Rashid, Tavga Sulaiman
Wong, Mui Yun
author_sort Awla, Hayman Kakakhan
title Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae
title_short Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae
title_full Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae
title_fullStr Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae
title_full_unstemmed Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae
title_sort bioactive compounds produced by streptomyces sp. isolate upmrs4 and antifungal activity against pyricularia oryzae
publisher Scientific Research Publishing
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/55200/1/Bioactive%20compounds%20produced%20by%20Streptomyces%20sp.%20isolate%20UPMRS4%20and%20antifungal%20activity%20against%20Pyricularia%20oryzae.pdf
http://psasir.upm.edu.my/id/eprint/55200/
http://www.scirp.org/journal/ajps
_version_ 1643835824725295104
score 13.211869

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