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Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds

A phenolic biosensor based on a zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds has been explored. The formation of the composite film was expected via electrostatic interaction between hexacetyltrimethylammonium bromide (CTAB), polyethylene glyc...

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Main Authors: Ahmad, Nor Monica, Abdullah, Jaafar, Yusof, Nor Azah, Ab Rashid, Ahmad Hazri, Abd Rahman, Samsulida, Hasan, Md. Rakibul
Format: Article
Language:English
Published: MDPI 2016
Online Access:http://psasir.upm.edu.my/id/eprint/55006/1/Amperometric%20Biosensor%20Based%20on%20Zirconium%20OxidePolyethylene%20GlycolTyrosinase%20Composite%20Film%20for%20the%20Detection%20of%20Phenolic%20Compounds.pdf
http://psasir.upm.edu.my/id/eprint/55006/
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spelling my.upm.eprints.550062018-07-12T10:30:35Z http://psasir.upm.edu.my/id/eprint/55006/ Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds Ahmad, Nor Monica Abdullah, Jaafar Yusof, Nor Azah Ab Rashid, Ahmad Hazri Abd Rahman, Samsulida Hasan, Md. Rakibul A phenolic biosensor based on a zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds has been explored. The formation of the composite film was expected via electrostatic interaction between hexacetyltrimethylammonium bromide (CTAB), polyethylene glycol (PEG), and zirconium oxide nanoparticles casted on screen printed carbon electrode (SPCE). Herein, the electrode was treated by casting hexacetyltrimethylammonium bromide on SPCE to promote a positively charged surface. Later, zirconium oxide was mixed with polyethylene glycol and the mixture was dropped cast onto the positively charged SPCE/CTAB. Tyrosinase was further immobilized onto the modified SPCE. Characterization of the prepared nanocomposite film and the modified SPCE surface was investigated by scanning electron microscopy (SEM), Electrochemical Impedance Spectroscopy (EIS), and Cyclic voltamogram (CV). The developed biosensor exhibits rapid response for less than 10 s. Two linear calibration curves towards phenol in the concentrations ranges of 0.075-10 µM and 10-55 µM with the detection limit of 0.034 µM were obtained. The biosensor shows high sensitivity and good storage stability for at least 30 days. MDPI 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/55006/1/Amperometric%20Biosensor%20Based%20on%20Zirconium%20OxidePolyethylene%20GlycolTyrosinase%20Composite%20Film%20for%20the%20Detection%20of%20Phenolic%20Compounds.pdf Ahmad, Nor Monica and Abdullah, Jaafar and Yusof, Nor Azah and Ab Rashid, Ahmad Hazri and Abd Rahman, Samsulida and Hasan, Md. Rakibul (2016) Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds. Biosensors, 6 (31). pp. 1-14. ISSN 2079-6374 10.3390/bios6030031
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A phenolic biosensor based on a zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds has been explored. The formation of the composite film was expected via electrostatic interaction between hexacetyltrimethylammonium bromide (CTAB), polyethylene glycol (PEG), and zirconium oxide nanoparticles casted on screen printed carbon electrode (SPCE). Herein, the electrode was treated by casting hexacetyltrimethylammonium bromide on SPCE to promote a positively charged surface. Later, zirconium oxide was mixed with polyethylene glycol and the mixture was dropped cast onto the positively charged SPCE/CTAB. Tyrosinase was further immobilized onto the modified SPCE. Characterization of the prepared nanocomposite film and the modified SPCE surface was investigated by scanning electron microscopy (SEM), Electrochemical Impedance Spectroscopy (EIS), and Cyclic voltamogram (CV). The developed biosensor exhibits rapid response for less than 10 s. Two linear calibration curves towards phenol in the concentrations ranges of 0.075-10 µM and 10-55 µM with the detection limit of 0.034 µM were obtained. The biosensor shows high sensitivity and good storage stability for at least 30 days.
format Article
author Ahmad, Nor Monica
Abdullah, Jaafar
Yusof, Nor Azah
Ab Rashid, Ahmad Hazri
Abd Rahman, Samsulida
Hasan, Md. Rakibul
spellingShingle Ahmad, Nor Monica
Abdullah, Jaafar
Yusof, Nor Azah
Ab Rashid, Ahmad Hazri
Abd Rahman, Samsulida
Hasan, Md. Rakibul
Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds
author_facet Ahmad, Nor Monica
Abdullah, Jaafar
Yusof, Nor Azah
Ab Rashid, Ahmad Hazri
Abd Rahman, Samsulida
Hasan, Md. Rakibul
author_sort Ahmad, Nor Monica
title Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds
title_short Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds
title_full Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds
title_fullStr Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds
title_full_unstemmed Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds
title_sort amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds
publisher MDPI
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/55006/1/Amperometric%20Biosensor%20Based%20on%20Zirconium%20OxidePolyethylene%20GlycolTyrosinase%20Composite%20Film%20for%20the%20Detection%20of%20Phenolic%20Compounds.pdf
http://psasir.upm.edu.my/id/eprint/55006/
_version_ 1643835771180810240
score 13.211869

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