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A low prevalence of inducible macrolide, lincosamide, and streptogramin B Resistance phenotype among methicillin-susceptible staphylococcus aureus isolated from Malaysian patients and healthy individuals

Background: Antibiotic resistance among Staphylococcus aureus is of great concern worldwide. This resistance is further complicated by the ability of S. aureus to confer cross-resistance to other antibiotics due to the presence of resistance genes, such as erythromycin resistance methylase (erm) gen...

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Main Authors: Elkammoshi, Asma Mesbah, Ghasemzadeh-Moghaddam, Hamed, Amin Nordin, Syafinaz, Mohd Taib, Niazlin, Subbiah, Suresh Kumar, Neela, Vasantha Kumari, Hamat, Rukman Awang
Format: Article
Language:English
Published: Ahvaz Jundishapur University of Medical Sciences 2016
Online Access:http://psasir.upm.edu.my/id/eprint/54855/1/A%20low%20prevalence%20of%20inducible%20macrolide%2C%20lincosamide%2C%20and%20streptogramin%20B%20Resistance%20.pdf
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spelling my.upm.eprints.548552018-05-22T01:47:31Z http://psasir.upm.edu.my/id/eprint/54855/ A low prevalence of inducible macrolide, lincosamide, and streptogramin B Resistance phenotype among methicillin-susceptible staphylococcus aureus isolated from Malaysian patients and healthy individuals Elkammoshi, Asma Mesbah Ghasemzadeh-Moghaddam, Hamed Amin Nordin, Syafinaz Mohd Taib, Niazlin Subbiah, Suresh Kumar Neela, Vasantha Kumari Hamat, Rukman Awang Background: Antibiotic resistance among Staphylococcus aureus is of great concern worldwide. This resistance is further complicated by the ability of S. aureus to confer cross-resistance to other antibiotics due to the presence of resistance genes, such as erythromycin resistance methylase (erm) genes, which render the bacterium resistant to macrolide-lincosamide-streptogramin B (MLSB) antibiotics. Resistance to these antibiotics can lead to therapeutic failure, resulting in significant morbidity and mortality in patients with S. aureus infections. Objectives: This study was performed to examine the distribution of MLSB-resistant strains of methicillin-susceptible S. aureus (MSSA), which were obtained from hospitalized patients and normal healthy individuals (carriers) using phenotypic methods, such as the double-disk diffusion (D-test) and the genotypic method by polymerase chain reaction (PCR). Methods: A total of 183 nonduplicative MSSA isolates obtained from hospitalized patients (133) and carriers (50) in our previous studies were randomly selected for the D-test. The guidelines of the Clinical and Laboratory Standards Institute (CLSI) were used for the interpretation of the results of this test. The detection of ermA, ermB, ermC and msrA genes by PCR was performed for isolates that had positive D-test results and that were resistant to erythromycin. Results: Of the 183 MSSA isolates, 97.2% and 98.4% were highly susceptible to erythromycin and clindamycin, respectively. MSLB resistance was detected in four isolates (2.2%). Of the 133 MSSA isolated from hospitalized patients, only 3.0% (4/133) and 2.3% (3/133) exhibited resistance to erythromycin and clindamycin, respectively. With regard to the MLSB resistance phenotypes, only 1.6% and 0.6% exhibited inducible MLSB (iMLSB) and MS phenotypes, respectively. The ermC gene was detected in all three iMLSB phenotypes, and the msrA gene was detected in the MS phenotype. Surprisingly, all MSSA isolates (100%) from carriers exhibited extremely high susceptibility to both antibiotics. Conclusions: The prevalence rates of iMLSB MSSA isolates vary according to geographical locations and the local antibiotic policy. The low prevalence rate of iMLSB MSSA isolates could probably be related to the judicious use of antibiotics for treating S. aureus infections in our studied population. Nonetheless, continuous antibiotic surveillance is still necessary to control any emergence of resistance isolates so that targeted therapy and effective control can be implemented accordingly. Ahvaz Jundishapur University of Medical Sciences 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54855/1/A%20low%20prevalence%20of%20inducible%20macrolide%2C%20lincosamide%2C%20and%20streptogramin%20B%20Resistance%20.pdf Elkammoshi, Asma Mesbah and Ghasemzadeh-Moghaddam, Hamed and Amin Nordin, Syafinaz and Mohd Taib, Niazlin and Subbiah, Suresh Kumar and Neela, Vasantha Kumari and Hamat, Rukman Awang (2016) A low prevalence of inducible macrolide, lincosamide, and streptogramin B Resistance phenotype among methicillin-susceptible staphylococcus aureus isolated from Malaysian patients and healthy individuals. Jundishapur Journal of Microbiology, 9 (10). pp. 1-7. ISSN 2008-3645; ESSN: 2008-4161 10.5812/jjm.37148
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: Antibiotic resistance among Staphylococcus aureus is of great concern worldwide. This resistance is further complicated by the ability of S. aureus to confer cross-resistance to other antibiotics due to the presence of resistance genes, such as erythromycin resistance methylase (erm) genes, which render the bacterium resistant to macrolide-lincosamide-streptogramin B (MLSB) antibiotics. Resistance to these antibiotics can lead to therapeutic failure, resulting in significant morbidity and mortality in patients with S. aureus infections. Objectives: This study was performed to examine the distribution of MLSB-resistant strains of methicillin-susceptible S. aureus (MSSA), which were obtained from hospitalized patients and normal healthy individuals (carriers) using phenotypic methods, such as the double-disk diffusion (D-test) and the genotypic method by polymerase chain reaction (PCR). Methods: A total of 183 nonduplicative MSSA isolates obtained from hospitalized patients (133) and carriers (50) in our previous studies were randomly selected for the D-test. The guidelines of the Clinical and Laboratory Standards Institute (CLSI) were used for the interpretation of the results of this test. The detection of ermA, ermB, ermC and msrA genes by PCR was performed for isolates that had positive D-test results and that were resistant to erythromycin. Results: Of the 183 MSSA isolates, 97.2% and 98.4% were highly susceptible to erythromycin and clindamycin, respectively. MSLB resistance was detected in four isolates (2.2%). Of the 133 MSSA isolated from hospitalized patients, only 3.0% (4/133) and 2.3% (3/133) exhibited resistance to erythromycin and clindamycin, respectively. With regard to the MLSB resistance phenotypes, only 1.6% and 0.6% exhibited inducible MLSB (iMLSB) and MS phenotypes, respectively. The ermC gene was detected in all three iMLSB phenotypes, and the msrA gene was detected in the MS phenotype. Surprisingly, all MSSA isolates (100%) from carriers exhibited extremely high susceptibility to both antibiotics. Conclusions: The prevalence rates of iMLSB MSSA isolates vary according to geographical locations and the local antibiotic policy. The low prevalence rate of iMLSB MSSA isolates could probably be related to the judicious use of antibiotics for treating S. aureus infections in our studied population. Nonetheless, continuous antibiotic surveillance is still necessary to control any emergence of resistance isolates so that targeted therapy and effective control can be implemented accordingly.
format Article
author Elkammoshi, Asma Mesbah
Ghasemzadeh-Moghaddam, Hamed
Amin Nordin, Syafinaz
Mohd Taib, Niazlin
Subbiah, Suresh Kumar
Neela, Vasantha Kumari
Hamat, Rukman Awang
spellingShingle Elkammoshi, Asma Mesbah
Ghasemzadeh-Moghaddam, Hamed
Amin Nordin, Syafinaz
Mohd Taib, Niazlin
Subbiah, Suresh Kumar
Neela, Vasantha Kumari
Hamat, Rukman Awang
A low prevalence of inducible macrolide, lincosamide, and streptogramin B Resistance phenotype among methicillin-susceptible staphylococcus aureus isolated from Malaysian patients and healthy individuals
author_facet Elkammoshi, Asma Mesbah
Ghasemzadeh-Moghaddam, Hamed
Amin Nordin, Syafinaz
Mohd Taib, Niazlin
Subbiah, Suresh Kumar
Neela, Vasantha Kumari
Hamat, Rukman Awang
author_sort Elkammoshi, Asma Mesbah
title A low prevalence of inducible macrolide, lincosamide, and streptogramin B Resistance phenotype among methicillin-susceptible staphylococcus aureus isolated from Malaysian patients and healthy individuals
title_short A low prevalence of inducible macrolide, lincosamide, and streptogramin B Resistance phenotype among methicillin-susceptible staphylococcus aureus isolated from Malaysian patients and healthy individuals
title_full A low prevalence of inducible macrolide, lincosamide, and streptogramin B Resistance phenotype among methicillin-susceptible staphylococcus aureus isolated from Malaysian patients and healthy individuals
title_fullStr A low prevalence of inducible macrolide, lincosamide, and streptogramin B Resistance phenotype among methicillin-susceptible staphylococcus aureus isolated from Malaysian patients and healthy individuals
title_full_unstemmed A low prevalence of inducible macrolide, lincosamide, and streptogramin B Resistance phenotype among methicillin-susceptible staphylococcus aureus isolated from Malaysian patients and healthy individuals
title_sort low prevalence of inducible macrolide, lincosamide, and streptogramin b resistance phenotype among methicillin-susceptible staphylococcus aureus isolated from malaysian patients and healthy individuals
publisher Ahvaz Jundishapur University of Medical Sciences
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/54855/1/A%20low%20prevalence%20of%20inducible%20macrolide%2C%20lincosamide%2C%20and%20streptogramin%20B%20Resistance%20.pdf
http://psasir.upm.edu.my/id/eprint/54855/
_version_ 1643835726381449216
score 13.244413

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