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Isolation and characterization of a molybdenum-reducing and amide-degrading Burkholderia sp. strain NENI-11 in soils from West Sumatra, Indonesia

A molybdenum-reducing bacterium isolated from contaminated soil was able to utilize acrylamide as the electron donor source, and was able utilize acrylamide, acetamide and propionamide for growth. Reduction was optimal at pH between 6.0 to 6.3, at temperatures of between 30 and 37 oC, glucose as the...

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Main Authors: Mansur, Rusnam, Gusmanizar, Neni, Dahalan, Farrah Aini, Masdor, Noor Azlina, Ahmad, Siti Aqlima, Shukor, Mohd. Shukri, Roslan, Muhamad Akhmal Hakim, Shukor, Mohd. Yunus
Format: Article
Language:English
Published: Institute of Integrative Omics and Applied Biotechnology 2016
Online Access:http://psasir.upm.edu.my/id/eprint/54630/1/Isolation%20and%20characterization%20of%20a%20molybdenum-reducing%20and%20amide-degrading%20Burkholderia%20.pdf
http://psasir.upm.edu.my/id/eprint/54630/
http://www.iioab.org/vol7n1.htm
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spelling my.upm.eprints.546302018-04-17T02:42:47Z http://psasir.upm.edu.my/id/eprint/54630/ Isolation and characterization of a molybdenum-reducing and amide-degrading Burkholderia sp. strain NENI-11 in soils from West Sumatra, Indonesia Mansur, Rusnam Gusmanizar, Neni Dahalan, Farrah Aini Masdor, Noor Azlina Ahmad, Siti Aqlima Shukor, Mohd. Shukri Roslan, Muhamad Akhmal Hakim Shukor, Mohd. Yunus A molybdenum-reducing bacterium isolated from contaminated soil was able to utilize acrylamide as the electron donor source, and was able utilize acrylamide, acetamide and propionamide for growth. Reduction was optimal at pH between 6.0 to 6.3, at temperatures of between 30 and 37 oC, glucose as the electron donor, phosphate at 5.0 mM, and sodium molybdate at 15 mM. The absorption spectrum of the Mo-blue indicates it is a reduced phosphomolybdate. Molybdenum reduction was inhibited by mercury (ii), silver (i) and chromium (vi) at 2 p.p.m. by 91.9, 82.7 and 17.4 %, respectively. Biochemical analysis resulted in a tentative identification of the bacterium as Burkholderia cepacia strain Neni-11. The growth of this bacterium modelled according to the modified Gompertz model. The growth parameters obtained were maximum specific growth rates of 1.241 d-1, 0.971 d-1, 0.85 d-1 for acrylamide, propionamide and acetamide, respectively, while the lag periods of 1.372 d, 1.562 and 1.639 d were observed for acrylamide, propionamide and acetamide, respectively. The ability of this bacterium to detoxify molybdenum and grown on toxic amides makes this bacterium an important tool for bioremediation. Institute of Integrative Omics and Applied Biotechnology 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54630/1/Isolation%20and%20characterization%20of%20a%20molybdenum-reducing%20and%20amide-degrading%20Burkholderia%20.pdf Mansur, Rusnam and Gusmanizar, Neni and Dahalan, Farrah Aini and Masdor, Noor Azlina and Ahmad, Siti Aqlima and Shukor, Mohd. Shukri and Roslan, Muhamad Akhmal Hakim and Shukor, Mohd. Yunus (2016) Isolation and characterization of a molybdenum-reducing and amide-degrading Burkholderia sp. strain NENI-11 in soils from West Sumatra, Indonesia. The IIOAB Journal, 7 (1). pp. 28-40. ISSN 0976-3104 http://www.iioab.org/vol7n1.htm
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A molybdenum-reducing bacterium isolated from contaminated soil was able to utilize acrylamide as the electron donor source, and was able utilize acrylamide, acetamide and propionamide for growth. Reduction was optimal at pH between 6.0 to 6.3, at temperatures of between 30 and 37 oC, glucose as the electron donor, phosphate at 5.0 mM, and sodium molybdate at 15 mM. The absorption spectrum of the Mo-blue indicates it is a reduced phosphomolybdate. Molybdenum reduction was inhibited by mercury (ii), silver (i) and chromium (vi) at 2 p.p.m. by 91.9, 82.7 and 17.4 %, respectively. Biochemical analysis resulted in a tentative identification of the bacterium as Burkholderia cepacia strain Neni-11. The growth of this bacterium modelled according to the modified Gompertz model. The growth parameters obtained were maximum specific growth rates of 1.241 d-1, 0.971 d-1, 0.85 d-1 for acrylamide, propionamide and acetamide, respectively, while the lag periods of 1.372 d, 1.562 and 1.639 d were observed for acrylamide, propionamide and acetamide, respectively. The ability of this bacterium to detoxify molybdenum and grown on toxic amides makes this bacterium an important tool for bioremediation.
format Article
author Mansur, Rusnam
Gusmanizar, Neni
Dahalan, Farrah Aini
Masdor, Noor Azlina
Ahmad, Siti Aqlima
Shukor, Mohd. Shukri
Roslan, Muhamad Akhmal Hakim
Shukor, Mohd. Yunus
spellingShingle Mansur, Rusnam
Gusmanizar, Neni
Dahalan, Farrah Aini
Masdor, Noor Azlina
Ahmad, Siti Aqlima
Shukor, Mohd. Shukri
Roslan, Muhamad Akhmal Hakim
Shukor, Mohd. Yunus
Isolation and characterization of a molybdenum-reducing and amide-degrading Burkholderia sp. strain NENI-11 in soils from West Sumatra, Indonesia
author_facet Mansur, Rusnam
Gusmanizar, Neni
Dahalan, Farrah Aini
Masdor, Noor Azlina
Ahmad, Siti Aqlima
Shukor, Mohd. Shukri
Roslan, Muhamad Akhmal Hakim
Shukor, Mohd. Yunus
author_sort Mansur, Rusnam
title Isolation and characterization of a molybdenum-reducing and amide-degrading Burkholderia sp. strain NENI-11 in soils from West Sumatra, Indonesia
title_short Isolation and characterization of a molybdenum-reducing and amide-degrading Burkholderia sp. strain NENI-11 in soils from West Sumatra, Indonesia
title_full Isolation and characterization of a molybdenum-reducing and amide-degrading Burkholderia sp. strain NENI-11 in soils from West Sumatra, Indonesia
title_fullStr Isolation and characterization of a molybdenum-reducing and amide-degrading Burkholderia sp. strain NENI-11 in soils from West Sumatra, Indonesia
title_full_unstemmed Isolation and characterization of a molybdenum-reducing and amide-degrading Burkholderia sp. strain NENI-11 in soils from West Sumatra, Indonesia
title_sort isolation and characterization of a molybdenum-reducing and amide-degrading burkholderia sp. strain neni-11 in soils from west sumatra, indonesia
publisher Institute of Integrative Omics and Applied Biotechnology
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/54630/1/Isolation%20and%20characterization%20of%20a%20molybdenum-reducing%20and%20amide-degrading%20Burkholderia%20.pdf
http://psasir.upm.edu.my/id/eprint/54630/
http://www.iioab.org/vol7n1.htm
_version_ 1643835675454210048
score 13.211869

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