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In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation

Blood disease is a wilt disease that greatly affects the banana cultivation. Besides chemicals, the utilization of natural soil-biological entity may provide an alternative in searching for effective and environmental friendly approach against the disease. Endogeic earthworms are known as soil-engin...

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Main Authors: Teng, Suk Kuan, Abd. Aziz, Nor Azwady, Mustafa, Muskhazli, Laboh, Rozeita, Ismail, Intan Safinar, Devi, Sujithra
Format: Article
Language:English
Published: Conscientia Beam 2016
Online Access:http://psasir.upm.edu.my/id/eprint/54621/1/In-vitro%20study%20on%20the%20effect%20of%20endogeic%20earthworm%20on%20blood%20disease%20bacterium%20.pdf
http://psasir.upm.edu.my/id/eprint/54621/
https://econpapers.repec.org/article/pkptajoam/2016_3ap_3a1-11.htm
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spelling my.upm.eprints.546212018-04-05T02:52:24Z http://psasir.upm.edu.my/id/eprint/54621/ In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation Teng, Suk Kuan Abd. Aziz, Nor Azwady Mustafa, Muskhazli Laboh, Rozeita Ismail, Intan Safinar Devi, Sujithra Blood disease is a wilt disease that greatly affects the banana cultivation. Besides chemicals, the utilization of natural soil-biological entity may provide an alternative in searching for effective and environmental friendly approach against the disease. Endogeic earthworms are known as soil-engineers that modify soil properties that may favor or suppress different microbial populations. In the study, the effect of endogeic earthworm, Pontoscolex corethrurus on blood disease bacterium (BDB) was evaluated through in-vitro study. Earthworm casts, mucus and worm-worked soil collected were inoculated onto tetrazolium chloride (TZC) medium seeded with BDB at different concentrations. Molecular identification using 16S rRNA primers was then performed to identify the microbial isolates that inhibited BDB. Phylogenetic tree was constructed to determine the similarity among the isolates with Pseudomonas sp. sequences deposited in the GenBank. The results showed that bacterial colonies from earthworm mucus inhibited BDB at 103 and 105 cell/ml. However, no inhibition was observed at higher BDB concentration (107 cell/ml). Similarly, earthworm cast and worm-worked soil did not show inhibition towards BDB. Bacterial isolates obtained from earthworm mucus were classified into the genus Pseudomonas. The study suggested the potential role of P. corethrurus in enhancing the growth of beneficial microorganism in remediating blood disease. Conscientia Beam 2016-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54621/1/In-vitro%20study%20on%20the%20effect%20of%20endogeic%20earthworm%20on%20blood%20disease%20bacterium%20.pdf Teng, Suk Kuan and Abd. Aziz, Nor Azwady and Mustafa, Muskhazli and Laboh, Rozeita and Ismail, Intan Safinar and Devi, Sujithra (2016) In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation. The Asia Journal of Applied Microbiology, 3 (1). pp. 1-11. ISSN 2409-2177; ESSN: 2313-8157 https://econpapers.repec.org/article/pkptajoam/2016_3ap_3a1-11.htm 10.18488/journal.33/2016.3.1/33.1.1.11
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Blood disease is a wilt disease that greatly affects the banana cultivation. Besides chemicals, the utilization of natural soil-biological entity may provide an alternative in searching for effective and environmental friendly approach against the disease. Endogeic earthworms are known as soil-engineers that modify soil properties that may favor or suppress different microbial populations. In the study, the effect of endogeic earthworm, Pontoscolex corethrurus on blood disease bacterium (BDB) was evaluated through in-vitro study. Earthworm casts, mucus and worm-worked soil collected were inoculated onto tetrazolium chloride (TZC) medium seeded with BDB at different concentrations. Molecular identification using 16S rRNA primers was then performed to identify the microbial isolates that inhibited BDB. Phylogenetic tree was constructed to determine the similarity among the isolates with Pseudomonas sp. sequences deposited in the GenBank. The results showed that bacterial colonies from earthworm mucus inhibited BDB at 103 and 105 cell/ml. However, no inhibition was observed at higher BDB concentration (107 cell/ml). Similarly, earthworm cast and worm-worked soil did not show inhibition towards BDB. Bacterial isolates obtained from earthworm mucus were classified into the genus Pseudomonas. The study suggested the potential role of P. corethrurus in enhancing the growth of beneficial microorganism in remediating blood disease.
format Article
author Teng, Suk Kuan
Abd. Aziz, Nor Azwady
Mustafa, Muskhazli
Laboh, Rozeita
Ismail, Intan Safinar
Devi, Sujithra
spellingShingle Teng, Suk Kuan
Abd. Aziz, Nor Azwady
Mustafa, Muskhazli
Laboh, Rozeita
Ismail, Intan Safinar
Devi, Sujithra
In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation
author_facet Teng, Suk Kuan
Abd. Aziz, Nor Azwady
Mustafa, Muskhazli
Laboh, Rozeita
Ismail, Intan Safinar
Devi, Sujithra
author_sort Teng, Suk Kuan
title In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation
title_short In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation
title_full In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation
title_fullStr In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation
title_full_unstemmed In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation
title_sort in-vitro study on the effect of endogeic earthworm on blood disease bacterium (bdb) in banana: a preliminary observation
publisher Conscientia Beam
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/54621/1/In-vitro%20study%20on%20the%20effect%20of%20endogeic%20earthworm%20on%20blood%20disease%20bacterium%20.pdf
http://psasir.upm.edu.my/id/eprint/54621/
https://econpapers.repec.org/article/pkptajoam/2016_3ap_3a1-11.htm
_version_ 1643835673974669312
score 13.211869

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