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In Vitro micropropagation of Acacia auriculiformis from selected juvenile sources

The effects of 6- Benzylaminopurine (BA), different basal medium, sucrose concentration and gelling agent were investigated for shoot induction and multiplication of Acacia auriculiformis. Nodal ex¬plants derived from 5-month-old seedlings yielded the highest shoot multiplication rate in Murashige a...

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Main Authors: Ismail, Haliza, Muniandi, Sures Kumar, Mohd Yusoff, Aziah, Hassan, Nor Hasnida, Ab Shukor, Nor Aini
Format: Article
Language:English
Published: Polish Academy of Sciences, Institute of Dendrology 2016
Online Access:http://psasir.upm.edu.my/id/eprint/54540/1/In%20Vitro%20micropropagation%20of%20Acacia%20auriculiformis%20from%20selected%20juvenile%20sources.pdf
http://psasir.upm.edu.my/id/eprint/54540/
http://www.idpan.poznan.pl/content-dendrobiolgy/v75/1684-75-157-165
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spelling my.upm.eprints.545402018-03-27T07:14:57Z http://psasir.upm.edu.my/id/eprint/54540/ In Vitro micropropagation of Acacia auriculiformis from selected juvenile sources Ismail, Haliza Muniandi, Sures Kumar Mohd Yusoff, Aziah Hassan, Nor Hasnida Ab Shukor, Nor Aini The effects of 6- Benzylaminopurine (BA), different basal medium, sucrose concentration and gelling agent were investigated for shoot induction and multiplication of Acacia auriculiformis. Nodal ex¬plants derived from 5-month-old seedlings yielded the highest shoot multiplication rate in Murashige and Skoog medium (MS) with 0.44 μM BA, 30 g/L sucrose and 2 g/L Gelrite. The highest mean number of shoots (10) and mean length of shoots (5.07mm) were also obtained in this medium. Qualitative obser¬vation of the shoots cultured in 0.44 μM BA were greener and vigorous in growth as compared to shoots cultured on higher concentrations of BA (22.2 μM). MS medium produced a significantly higher number of shoots (18) compared to Woody Plant Medium (WPM) (11) and B5 medium (10). Media solidified with different gelling agents also produced a significantly different number of shoots with 2 g/L Gelrite produced the highest number of shoots (23). The highest percentage of shoots rooted was found in the MS medium without any growth regulators (40.0%) followed by medium supplemented with Indole-3-bu¬tyric acid (IBA) at 9.84 μM and the combination of 9.84 μM IBA with 5.37 μM α-naphthalene acetic acid (NAA) (33.3%). MS medium without any plant growth regulators produced the highest mean root length (84.33mm), whereas medium supplemented with 9.84 μM IBA produced the highest mean number of roots per shoot (4.33). Out planting of in vitro rooted shoots in shredded coconut husk as the substrate gave the highest percentage of survival (90%) during acclimatization in the greenhouse. Polish Academy of Sciences, Institute of Dendrology 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54540/1/In%20Vitro%20micropropagation%20of%20Acacia%20auriculiformis%20from%20selected%20juvenile%20sources.pdf Ismail, Haliza and Muniandi, Sures Kumar and Mohd Yusoff, Aziah and Hassan, Nor Hasnida and Ab Shukor, Nor Aini (2016) In Vitro micropropagation of Acacia auriculiformis from selected juvenile sources. Dendrobiology, 75. pp. 157-165. ISSN 1641-1307 http://www.idpan.poznan.pl/content-dendrobiolgy/v75/1684-75-157-165 10.12657/denbio.075.015
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The effects of 6- Benzylaminopurine (BA), different basal medium, sucrose concentration and gelling agent were investigated for shoot induction and multiplication of Acacia auriculiformis. Nodal ex¬plants derived from 5-month-old seedlings yielded the highest shoot multiplication rate in Murashige and Skoog medium (MS) with 0.44 μM BA, 30 g/L sucrose and 2 g/L Gelrite. The highest mean number of shoots (10) and mean length of shoots (5.07mm) were also obtained in this medium. Qualitative obser¬vation of the shoots cultured in 0.44 μM BA were greener and vigorous in growth as compared to shoots cultured on higher concentrations of BA (22.2 μM). MS medium produced a significantly higher number of shoots (18) compared to Woody Plant Medium (WPM) (11) and B5 medium (10). Media solidified with different gelling agents also produced a significantly different number of shoots with 2 g/L Gelrite produced the highest number of shoots (23). The highest percentage of shoots rooted was found in the MS medium without any growth regulators (40.0%) followed by medium supplemented with Indole-3-bu¬tyric acid (IBA) at 9.84 μM and the combination of 9.84 μM IBA with 5.37 μM α-naphthalene acetic acid (NAA) (33.3%). MS medium without any plant growth regulators produced the highest mean root length (84.33mm), whereas medium supplemented with 9.84 μM IBA produced the highest mean number of roots per shoot (4.33). Out planting of in vitro rooted shoots in shredded coconut husk as the substrate gave the highest percentage of survival (90%) during acclimatization in the greenhouse.
format Article
author Ismail, Haliza
Muniandi, Sures Kumar
Mohd Yusoff, Aziah
Hassan, Nor Hasnida
Ab Shukor, Nor Aini
spellingShingle Ismail, Haliza
Muniandi, Sures Kumar
Mohd Yusoff, Aziah
Hassan, Nor Hasnida
Ab Shukor, Nor Aini
In Vitro micropropagation of Acacia auriculiformis from selected juvenile sources
author_facet Ismail, Haliza
Muniandi, Sures Kumar
Mohd Yusoff, Aziah
Hassan, Nor Hasnida
Ab Shukor, Nor Aini
author_sort Ismail, Haliza
title In Vitro micropropagation of Acacia auriculiformis from selected juvenile sources
title_short In Vitro micropropagation of Acacia auriculiformis from selected juvenile sources
title_full In Vitro micropropagation of Acacia auriculiformis from selected juvenile sources
title_fullStr In Vitro micropropagation of Acacia auriculiformis from selected juvenile sources
title_full_unstemmed In Vitro micropropagation of Acacia auriculiformis from selected juvenile sources
title_sort in vitro micropropagation of acacia auriculiformis from selected juvenile sources
publisher Polish Academy of Sciences, Institute of Dendrology
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/54540/1/In%20Vitro%20micropropagation%20of%20Acacia%20auriculiformis%20from%20selected%20juvenile%20sources.pdf
http://psasir.upm.edu.my/id/eprint/54540/
http://www.idpan.poznan.pl/content-dendrobiolgy/v75/1684-75-157-165
_version_ 1643835657600106496
score 13.211869

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