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Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol

Nanocrystalline cellulose (NCC)/chitosan composite for immobilization of tyrosinase enzyme for the determination of phenol have been developed. The NCC/Chitosan composite film on screen printed carbon electrode (SPCE) was prepared by using drop casting technique. Characterization of the modified SPC...

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Main Authors: Abd Manan, Fariza Aina, Abdullah, Jaafar, Nazri, Nur Nadziera, Abd Malik, Izyan Nadira, Yusof, Nor Azah, Ahmad, Ishak
Format: Article
Language:English
Published: Malaysian Analytical Sciences Society 2016
Online Access:http://psasir.upm.edu.my/id/eprint/54496/1/Immobilization%20of%20tyrosinase%20in%20nanocrystalline%20cellulose.pdf
http://psasir.upm.edu.my/id/eprint/54496/
http://www.ukm.my/mjas/mjas2018/
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spelling my.upm.eprints.544962018-03-21T03:22:07Z http://psasir.upm.edu.my/id/eprint/54496/ Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol Abd Manan, Fariza Aina Abdullah, Jaafar Nazri, Nur Nadziera Abd Malik, Izyan Nadira Yusof, Nor Azah Ahmad, Ishak Nanocrystalline cellulose (NCC)/chitosan composite for immobilization of tyrosinase enzyme for the determination of phenol have been developed. The NCC/Chitosan composite film on screen printed carbon electrode (SPCE) was prepared by using drop casting technique. Characterization of the modified SPCE surface was investigated by using Transmission Electron Microscopy (TEM) and Fourier Transform Infrared (FTIR), respectively. Chronoamperometric (CA) technique is used to perform the electrochemical measurements. The detection of phenols by the developed system is derived on the direct electrochemical reduction of quinones produced by enzymatic reaction. The results demonstrated that the maximum response was observed at ratio of NCC/chitosan of 1 to 1 (v/v), tyrosinase concentration of 10 mg/mL and pH buffer of 7, respectively. It was found that the developed system gave linear response in the phenol concentration range of 0.39 – 7.74 µM (slope = 28.316, R2 = 0.9808) with the detection limit of 0.38 µM. The reproducibility of the system was also estimated and the Relative Standard Deviation (RSD) was found to be at 4.27%. Malaysian Analytical Sciences Society 2016-10 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54496/1/Immobilization%20of%20tyrosinase%20in%20nanocrystalline%20cellulose.pdf Abd Manan, Fariza Aina and Abdullah, Jaafar and Nazri, Nur Nadziera and Abd Malik, Izyan Nadira and Yusof, Nor Azah and Ahmad, Ishak (2016) Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol. Malaysian Journal of Analytical Sciences, 20 (5). pp. 978-985. ISSN 1394-2506 http://www.ukm.my/mjas/mjas2018/ 10.17576/mjas-2016-2005-02
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Nanocrystalline cellulose (NCC)/chitosan composite for immobilization of tyrosinase enzyme for the determination of phenol have been developed. The NCC/Chitosan composite film on screen printed carbon electrode (SPCE) was prepared by using drop casting technique. Characterization of the modified SPCE surface was investigated by using Transmission Electron Microscopy (TEM) and Fourier Transform Infrared (FTIR), respectively. Chronoamperometric (CA) technique is used to perform the electrochemical measurements. The detection of phenols by the developed system is derived on the direct electrochemical reduction of quinones produced by enzymatic reaction. The results demonstrated that the maximum response was observed at ratio of NCC/chitosan of 1 to 1 (v/v), tyrosinase concentration of 10 mg/mL and pH buffer of 7, respectively. It was found that the developed system gave linear response in the phenol concentration range of 0.39 – 7.74 µM (slope = 28.316, R2 = 0.9808) with the detection limit of 0.38 µM. The reproducibility of the system was also estimated and the Relative Standard Deviation (RSD) was found to be at 4.27%.
format Article
author Abd Manan, Fariza Aina
Abdullah, Jaafar
Nazri, Nur Nadziera
Abd Malik, Izyan Nadira
Yusof, Nor Azah
Ahmad, Ishak
spellingShingle Abd Manan, Fariza Aina
Abdullah, Jaafar
Nazri, Nur Nadziera
Abd Malik, Izyan Nadira
Yusof, Nor Azah
Ahmad, Ishak
Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol
author_facet Abd Manan, Fariza Aina
Abdullah, Jaafar
Nazri, Nur Nadziera
Abd Malik, Izyan Nadira
Yusof, Nor Azah
Ahmad, Ishak
author_sort Abd Manan, Fariza Aina
title Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol
title_short Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol
title_full Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol
title_fullStr Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol
title_full_unstemmed Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol
title_sort immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol
publisher Malaysian Analytical Sciences Society
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/54496/1/Immobilization%20of%20tyrosinase%20in%20nanocrystalline%20cellulose.pdf
http://psasir.upm.edu.my/id/eprint/54496/
http://www.ukm.my/mjas/mjas2018/
_version_ 1643835649204158464
score 13.211869

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