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Hepatoprotective action of various partitions of methanol extract of Bauhinia purpurea leaves against paracetamol-induced liver toxicity: involvement of the antioxidant mechanisms

Background: Methanol extract of Bauhinia purpurea L. (family Fabaceae) (MEBP) possesses high antioxidant and anti-inflammatory activities and recently reported to exert hepatoprotection against paracetamol (PCM)-induced liver injury in rats. In an attempt to identify the hepatoprotective bioactive c...

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Main Authors: Zakaria, Zainul Amiruddin, Yahya, Farhana, Mamat, Siti Syariah, Mahmood, Nur Diyana, Mohtarrudin, Nurhafizah, Taher, Muhammad, Abdul Hamid, Siti Selina, Lay, Kek Teh, Salleh, Mohd. Zaki
Format: Article
Language:English
Published: BioMed Central 2016
Online Access:http://psasir.upm.edu.my/id/eprint/54429/1/Hepatoprotective%20action%20of%20various%20partitions%20of%20methanol%20extract.pdf
http://psasir.upm.edu.my/id/eprint/54429/
https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-016-1110-4
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spelling my.upm.eprints.544292018-03-16T02:33:24Z http://psasir.upm.edu.my/id/eprint/54429/ Hepatoprotective action of various partitions of methanol extract of Bauhinia purpurea leaves against paracetamol-induced liver toxicity: involvement of the antioxidant mechanisms Zakaria, Zainul Amiruddin Yahya, Farhana Mamat, Siti Syariah Mahmood, Nur Diyana Mohtarrudin, Nurhafizah Taher, Muhammad Abdul Hamid, Siti Selina Lay, Kek Teh Salleh, Mohd. Zaki Background: Methanol extract of Bauhinia purpurea L. (family Fabaceae) (MEBP) possesses high antioxidant and anti-inflammatory activities and recently reported to exert hepatoprotection against paracetamol (PCM)-induced liver injury in rats. In an attempt to identify the hepatoprotective bioactive compounds in MEBP, the extract was prepared in different partitions and subjected to the PCM-induced liver injury model in rats. Methods: Dried MEBP was partitioned successively to obtain petroleum ether (PEBP), ethylacetate (EABP) and aqueous (AQBP) partitions, respectively. All partitions were subjected to in vitro antioxidant (i.e. total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH)- and superoxide-radicals scavenging assay, and oxygen radical absorbance capacity (ORAC) assay) and anti-inflammatory (i.e. lipooxygenase (LOX) and xanthine oxidase (XO) assay) analysis. The partitions, prepared in the dose range of 50, 250 and 500 mg/kg, together with a vehicle (10 % DMSO) and standard drug (200 mg/kg silymarin) were administered orally for 7 consecutive days prior to subjection to the 3 mg/kg PCM-induced liver injury model in rats. Following the hepatic injury induction, blood samples and liver were collected for the respective biochemical parameter and histopathological studies. Body weight changes and liver weight were also recorded. The partitions were also subjected to the phytochemical screening and HPLC analysis. Results: Of all partitions, EABP possessed high TPC value and demonstrated remarkable antioxidant activity when assessed using the DPPH- and superoxide-radical scavenging assay, as well as ORAC assay, which was followed by AQBP and PEBP. All partitions also showed low anti-inflammatory activity via the LOX and XO pathways. In the hepatoprotective study, the effectiveness of the partitions is in the order of EABP>AQBP>PEBP, which is supported by the microscopic analysis and histopathological scoring. In the biochemical analysis, EABP also exerted the most effective effect by reducing the serum level of alanine transaminase (ALT) and aspartate transaminase (AST) at all doses tested in comparison to the other partitions. Phytochemical screening and HPLC analysis suggested the presence of: flavonoids, condensed tannins and triterpenes in EABP; flavonoids, condensed tannins and saponins in PEBP and; only saponins in AQBP. Conclusion: EABP demonstrates the most effective hepatoprotection against PCM-induced liver injury in rats. This observation could be attributed to its remarkable antioxidant activity and the presence of flavonoids that might probably act synergistically with other biocompounds to cause the hepatoprotection. BioMed Central 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54429/1/Hepatoprotective%20action%20of%20various%20partitions%20of%20methanol%20extract.pdf Zakaria, Zainul Amiruddin and Yahya, Farhana and Mamat, Siti Syariah and Mahmood, Nur Diyana and Mohtarrudin, Nurhafizah and Taher, Muhammad and Abdul Hamid, Siti Selina and Lay, Kek Teh and Salleh, Mohd. Zaki (2016) Hepatoprotective action of various partitions of methanol extract of Bauhinia purpurea leaves against paracetamol-induced liver toxicity: involvement of the antioxidant mechanisms. BMC Complementary and Alternative Medicine, 16 (175). pp. 1-16. ISSN 1472-6882 https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-016-1110-4 10.1186/s12906-016-1110-4
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: Methanol extract of Bauhinia purpurea L. (family Fabaceae) (MEBP) possesses high antioxidant and anti-inflammatory activities and recently reported to exert hepatoprotection against paracetamol (PCM)-induced liver injury in rats. In an attempt to identify the hepatoprotective bioactive compounds in MEBP, the extract was prepared in different partitions and subjected to the PCM-induced liver injury model in rats. Methods: Dried MEBP was partitioned successively to obtain petroleum ether (PEBP), ethylacetate (EABP) and aqueous (AQBP) partitions, respectively. All partitions were subjected to in vitro antioxidant (i.e. total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH)- and superoxide-radicals scavenging assay, and oxygen radical absorbance capacity (ORAC) assay) and anti-inflammatory (i.e. lipooxygenase (LOX) and xanthine oxidase (XO) assay) analysis. The partitions, prepared in the dose range of 50, 250 and 500 mg/kg, together with a vehicle (10 % DMSO) and standard drug (200 mg/kg silymarin) were administered orally for 7 consecutive days prior to subjection to the 3 mg/kg PCM-induced liver injury model in rats. Following the hepatic injury induction, blood samples and liver were collected for the respective biochemical parameter and histopathological studies. Body weight changes and liver weight were also recorded. The partitions were also subjected to the phytochemical screening and HPLC analysis. Results: Of all partitions, EABP possessed high TPC value and demonstrated remarkable antioxidant activity when assessed using the DPPH- and superoxide-radical scavenging assay, as well as ORAC assay, which was followed by AQBP and PEBP. All partitions also showed low anti-inflammatory activity via the LOX and XO pathways. In the hepatoprotective study, the effectiveness of the partitions is in the order of EABP>AQBP>PEBP, which is supported by the microscopic analysis and histopathological scoring. In the biochemical analysis, EABP also exerted the most effective effect by reducing the serum level of alanine transaminase (ALT) and aspartate transaminase (AST) at all doses tested in comparison to the other partitions. Phytochemical screening and HPLC analysis suggested the presence of: flavonoids, condensed tannins and triterpenes in EABP; flavonoids, condensed tannins and saponins in PEBP and; only saponins in AQBP. Conclusion: EABP demonstrates the most effective hepatoprotection against PCM-induced liver injury in rats. This observation could be attributed to its remarkable antioxidant activity and the presence of flavonoids that might probably act synergistically with other biocompounds to cause the hepatoprotection.
format Article
author Zakaria, Zainul Amiruddin
Yahya, Farhana
Mamat, Siti Syariah
Mahmood, Nur Diyana
Mohtarrudin, Nurhafizah
Taher, Muhammad
Abdul Hamid, Siti Selina
Lay, Kek Teh
Salleh, Mohd. Zaki
spellingShingle Zakaria, Zainul Amiruddin
Yahya, Farhana
Mamat, Siti Syariah
Mahmood, Nur Diyana
Mohtarrudin, Nurhafizah
Taher, Muhammad
Abdul Hamid, Siti Selina
Lay, Kek Teh
Salleh, Mohd. Zaki
Hepatoprotective action of various partitions of methanol extract of Bauhinia purpurea leaves against paracetamol-induced liver toxicity: involvement of the antioxidant mechanisms
author_facet Zakaria, Zainul Amiruddin
Yahya, Farhana
Mamat, Siti Syariah
Mahmood, Nur Diyana
Mohtarrudin, Nurhafizah
Taher, Muhammad
Abdul Hamid, Siti Selina
Lay, Kek Teh
Salleh, Mohd. Zaki
author_sort Zakaria, Zainul Amiruddin
title Hepatoprotective action of various partitions of methanol extract of Bauhinia purpurea leaves against paracetamol-induced liver toxicity: involvement of the antioxidant mechanisms
title_short Hepatoprotective action of various partitions of methanol extract of Bauhinia purpurea leaves against paracetamol-induced liver toxicity: involvement of the antioxidant mechanisms
title_full Hepatoprotective action of various partitions of methanol extract of Bauhinia purpurea leaves against paracetamol-induced liver toxicity: involvement of the antioxidant mechanisms
title_fullStr Hepatoprotective action of various partitions of methanol extract of Bauhinia purpurea leaves against paracetamol-induced liver toxicity: involvement of the antioxidant mechanisms
title_full_unstemmed Hepatoprotective action of various partitions of methanol extract of Bauhinia purpurea leaves against paracetamol-induced liver toxicity: involvement of the antioxidant mechanisms
title_sort hepatoprotective action of various partitions of methanol extract of bauhinia purpurea leaves against paracetamol-induced liver toxicity: involvement of the antioxidant mechanisms
publisher BioMed Central
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/54429/1/Hepatoprotective%20action%20of%20various%20partitions%20of%20methanol%20extract.pdf
http://psasir.upm.edu.my/id/eprint/54429/
https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-016-1110-4
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score 13.211869

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