Facile modulation of enantioselectivity of thermophilic Geobacillus zalihae lipase by regulating hydrophobicity of its Q114 oxyanion
Site-directed mutagenesis of the oxyanion-containing amino acid Q114 in the recombinant thermophilic T1 lipase previously isolated from Geobacillus zalihae was performed to elucidate its role in the enzyme⿿s enantioselectivity and reactivity. Substitution of Q114 with a hydrophobic methionine to yie...
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| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54264/1/Facile%20modulation%20of%20enantioselectivity%20of%20thermophilic%20Geobacillus%20zalihae%20lipase%20by%20regulating%20hydrophobicity%20of%20its%20Q114%20oxyanion.pdf http://psasir.upm.edu.my/id/eprint/54264/ https://www.sciencedirect.com/science/article/pii/S0141022916301715 |
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http://psasir.upm.edu.my/id/eprint/54264/1/Facile%20modulation%20of%20enantioselectivity%20of%20thermophilic%20Geobacillus%20zalihae%20lipase%20by%20regulating%20hydrophobicity%20of%20its%20Q114%20oxyanion.pdfhttp://psasir.upm.edu.my/id/eprint/54264/
https://www.sciencedirect.com/science/article/pii/S0141022916301715