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Electrochemical detection of uric acid via uricase-immobilized graphene oxide

Measurement of the uric acid level in the body can be improved by biosensing with respect to the accuracy, sensitivity and time consumption. This study has reported the immobilization of uricase onto graphene oxide (GO) and its function for electrochemical detection of uric acid. Through chemical mo...

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Main Authors: Omar, Muhamad Nadzmi, Salleh, Abu Bakar, Lim, Hong Ngee, Ahmad Tajudin, Asilah
Format: Article
Language:English
Published: Elsevier 2016
Online Access:http://psasir.upm.edu.my/id/eprint/54142/1/Electrochemical%20detection%20of%20uric%20acid%20via%20uricase-immobilized%20graphene%20oxide.pdf
http://psasir.upm.edu.my/id/eprint/54142/
https://www.sciencedirect.com/science/article/pii/S0003269716301658
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spelling my.upm.eprints.541422018-03-01T02:00:04Z http://psasir.upm.edu.my/id/eprint/54142/ Electrochemical detection of uric acid via uricase-immobilized graphene oxide Omar, Muhamad Nadzmi Salleh, Abu Bakar Lim, Hong Ngee Ahmad Tajudin, Asilah Measurement of the uric acid level in the body can be improved by biosensing with respect to the accuracy, sensitivity and time consumption. This study has reported the immobilization of uricase onto graphene oxide (GO) and its function for electrochemical detection of uric acid. Through chemical modification of GO using 1-ethyl-3-(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysulfosuccinimide (NHS) as cross-linking reagents, the enzyme activity of the immobilized uricase was much comparable to the free enzyme with 88% of the activity retained. The modified GO-uricase (GOU) was then subjected to electrocatalytic detection of uric acid (UA) via cyclic voltammetry (CV). For that reason, a glassy carbon electrode (GCE) was modified by adhering the GO along with the immobilized uricase to facilitate the redox reaction between the enzyme and the substrate. The modified GOU/GCE outperformed a bare electrode through the electrocatalytic activity with an amplified electrical signal for the detection of UA. The electrocatalytic response showed a linear dependence on the UA concentration ranging from 0.02 to 0.49 mM with a detection limit of 3.45 μM at 3σ/m. The resulting biosensor also exhibited a high selectivity towards UA in the presence of other interference as well as good reproducibility. Elsevier 2016-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54142/1/Electrochemical%20detection%20of%20uric%20acid%20via%20uricase-immobilized%20graphene%20oxide.pdf Omar, Muhamad Nadzmi and Salleh, Abu Bakar and Lim, Hong Ngee and Ahmad Tajudin, Asilah (2016) Electrochemical detection of uric acid via uricase-immobilized graphene oxide. Analytical Biochemistry, 509. pp. 135-141. ISSN 0003-2697; ESSN: 1096-0309 https://www.sciencedirect.com/science/article/pii/S0003269716301658 10.1016/j.ab.2016.06.030
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Measurement of the uric acid level in the body can be improved by biosensing with respect to the accuracy, sensitivity and time consumption. This study has reported the immobilization of uricase onto graphene oxide (GO) and its function for electrochemical detection of uric acid. Through chemical modification of GO using 1-ethyl-3-(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysulfosuccinimide (NHS) as cross-linking reagents, the enzyme activity of the immobilized uricase was much comparable to the free enzyme with 88% of the activity retained. The modified GO-uricase (GOU) was then subjected to electrocatalytic detection of uric acid (UA) via cyclic voltammetry (CV). For that reason, a glassy carbon electrode (GCE) was modified by adhering the GO along with the immobilized uricase to facilitate the redox reaction between the enzyme and the substrate. The modified GOU/GCE outperformed a bare electrode through the electrocatalytic activity with an amplified electrical signal for the detection of UA. The electrocatalytic response showed a linear dependence on the UA concentration ranging from 0.02 to 0.49 mM with a detection limit of 3.45 μM at 3σ/m. The resulting biosensor also exhibited a high selectivity towards UA in the presence of other interference as well as good reproducibility.
format Article
author Omar, Muhamad Nadzmi
Salleh, Abu Bakar
Lim, Hong Ngee
Ahmad Tajudin, Asilah
spellingShingle Omar, Muhamad Nadzmi
Salleh, Abu Bakar
Lim, Hong Ngee
Ahmad Tajudin, Asilah
Electrochemical detection of uric acid via uricase-immobilized graphene oxide
author_facet Omar, Muhamad Nadzmi
Salleh, Abu Bakar
Lim, Hong Ngee
Ahmad Tajudin, Asilah
author_sort Omar, Muhamad Nadzmi
title Electrochemical detection of uric acid via uricase-immobilized graphene oxide
title_short Electrochemical detection of uric acid via uricase-immobilized graphene oxide
title_full Electrochemical detection of uric acid via uricase-immobilized graphene oxide
title_fullStr Electrochemical detection of uric acid via uricase-immobilized graphene oxide
title_full_unstemmed Electrochemical detection of uric acid via uricase-immobilized graphene oxide
title_sort electrochemical detection of uric acid via uricase-immobilized graphene oxide
publisher Elsevier
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/54142/1/Electrochemical%20detection%20of%20uric%20acid%20via%20uricase-immobilized%20graphene%20oxide.pdf
http://psasir.upm.edu.my/id/eprint/54142/
https://www.sciencedirect.com/science/article/pii/S0003269716301658
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score 13.211869

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