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Efficacy of potential biocontrol agents for control of Fusarium verticillioides and fumonisin B1 under different environmental conditions

A mycotoxigenic strain of Fusarium verticillioides previously isolated from Malaysian maize kernels and identified morphologically and molecularly was used in the present work. The objectives were (1) to screen the competitiveness of three potential antagonists isolated from Malaysian maize and thre...

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Main Authors: Samsudin, Nik Iskandar Putra, Magan, Naresh
Format: Article
Language:English
Published: Wageningen Academic Publishers 2016
Online Access:http://psasir.upm.edu.my/id/eprint/54123/1/Efficacy%20of%20potential%20biocontrol%20agents%20for%20control%20of%20Fusarium%20verticillioides%20.pdf
http://psasir.upm.edu.my/id/eprint/54123/
http://www.wageningenacademic.com/doi/abs/10.3920/WMJ2015.1886
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spelling my.upm.eprints.541232018-02-28T08:35:33Z http://psasir.upm.edu.my/id/eprint/54123/ Efficacy of potential biocontrol agents for control of Fusarium verticillioides and fumonisin B1 under different environmental conditions Samsudin, Nik Iskandar Putra Magan, Naresh A mycotoxigenic strain of Fusarium verticillioides previously isolated from Malaysian maize kernels and identified morphologically and molecularly was used in the present work. The objectives were (1) to screen the competitiveness of three potential antagonists isolated from Malaysian maize and three other known candidates for control of growth of F. verticillioides in vitro based on interaction scores, growth rates and hyphal area of F. verticillioides, and (2) to examine the best candidates using different spore/cell ratios on milled maize agar at different water activity conditions on relative control of fumonisin B1 (FB1). Three fungi and three bacteria (BCAs 1-6) were examined for antagonistic effects against F. verticillioides in dual-culture assays. These showed that all fungal candidates intermingled with F. verticillioides while all bacterial candidates inhibited F. verticillioides on contact or at a distance, which in turn decreased the growth rates and hyphal area of F. verticillioides significantly. Although BCA1 (Clonostachys rosea 016) did not inhibit growth or hyphal area of F. verticillioides, it was included in FB1 inhibition studies with other bacterial candidates (BCA4, Streptomyces sp. AS1; BCA5, Gram-negative rod; BCA6, Enterobacter hormaechei) because of its established mycoparasitism. The FB1 inhibition studies were conducted on milled maize agar with different spore/cell ratios of pathogen:antagonist mixtures at 0.95/0.98 water activity (aw) and 25 °C for 14 days. FB1 biosynthesis for all treatments was significantly higher at 0.95 than 0.98 aw. Of the four antagonists tested, the best was BCA1 which inhibited FB1 biosynthesis by 73 and 100% at 0.95 and 0.98 aw, respectively. BCA5 was the next best, resulting in 38 and 78% FB1 inhibition at 0.95 and 0.98 aw, respectively. The pathogen:antagonist ratios for BCA1 and BCA5 showed best results at 50:50 and 25:75. These results were discussed in context of using biocontrol agents to minimise fumonisins in maize. Wageningen Academic Publishers 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54123/1/Efficacy%20of%20potential%20biocontrol%20agents%20for%20control%20of%20Fusarium%20verticillioides%20.pdf Samsudin, Nik Iskandar Putra and Magan, Naresh (2016) Efficacy of potential biocontrol agents for control of Fusarium verticillioides and fumonisin B1 under different environmental conditions. World Mycotoxin Journal, 9 (2). pp. 205-213. ISSN 1875-0710; ESSN: 1875-0796 http://www.wageningenacademic.com/doi/abs/10.3920/WMJ2015.1886 10.3920/WMJ2015.1886
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A mycotoxigenic strain of Fusarium verticillioides previously isolated from Malaysian maize kernels and identified morphologically and molecularly was used in the present work. The objectives were (1) to screen the competitiveness of three potential antagonists isolated from Malaysian maize and three other known candidates for control of growth of F. verticillioides in vitro based on interaction scores, growth rates and hyphal area of F. verticillioides, and (2) to examine the best candidates using different spore/cell ratios on milled maize agar at different water activity conditions on relative control of fumonisin B1 (FB1). Three fungi and three bacteria (BCAs 1-6) were examined for antagonistic effects against F. verticillioides in dual-culture assays. These showed that all fungal candidates intermingled with F. verticillioides while all bacterial candidates inhibited F. verticillioides on contact or at a distance, which in turn decreased the growth rates and hyphal area of F. verticillioides significantly. Although BCA1 (Clonostachys rosea 016) did not inhibit growth or hyphal area of F. verticillioides, it was included in FB1 inhibition studies with other bacterial candidates (BCA4, Streptomyces sp. AS1; BCA5, Gram-negative rod; BCA6, Enterobacter hormaechei) because of its established mycoparasitism. The FB1 inhibition studies were conducted on milled maize agar with different spore/cell ratios of pathogen:antagonist mixtures at 0.95/0.98 water activity (aw) and 25 °C for 14 days. FB1 biosynthesis for all treatments was significantly higher at 0.95 than 0.98 aw. Of the four antagonists tested, the best was BCA1 which inhibited FB1 biosynthesis by 73 and 100% at 0.95 and 0.98 aw, respectively. BCA5 was the next best, resulting in 38 and 78% FB1 inhibition at 0.95 and 0.98 aw, respectively. The pathogen:antagonist ratios for BCA1 and BCA5 showed best results at 50:50 and 25:75. These results were discussed in context of using biocontrol agents to minimise fumonisins in maize.
format Article
author Samsudin, Nik Iskandar Putra
Magan, Naresh
spellingShingle Samsudin, Nik Iskandar Putra
Magan, Naresh
Efficacy of potential biocontrol agents for control of Fusarium verticillioides and fumonisin B1 under different environmental conditions
author_facet Samsudin, Nik Iskandar Putra
Magan, Naresh
author_sort Samsudin, Nik Iskandar Putra
title Efficacy of potential biocontrol agents for control of Fusarium verticillioides and fumonisin B1 under different environmental conditions
title_short Efficacy of potential biocontrol agents for control of Fusarium verticillioides and fumonisin B1 under different environmental conditions
title_full Efficacy of potential biocontrol agents for control of Fusarium verticillioides and fumonisin B1 under different environmental conditions
title_fullStr Efficacy of potential biocontrol agents for control of Fusarium verticillioides and fumonisin B1 under different environmental conditions
title_full_unstemmed Efficacy of potential biocontrol agents for control of Fusarium verticillioides and fumonisin B1 under different environmental conditions
title_sort efficacy of potential biocontrol agents for control of fusarium verticillioides and fumonisin b1 under different environmental conditions
publisher Wageningen Academic Publishers
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/54123/1/Efficacy%20of%20potential%20biocontrol%20agents%20for%20control%20of%20Fusarium%20verticillioides%20.pdf
http://psasir.upm.edu.my/id/eprint/54123/
http://www.wageningenacademic.com/doi/abs/10.3920/WMJ2015.1886
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score 13.211869

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