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Construction of PHB and PHBV multiple-gene vectors driven by an oil palm leaf-specific promoter

One of the targets in oil palm genetic engineering programme is the production of polyhydroxybutyrate (PHB) and polyhydroxybutyrate-co-valerate (PHBV) in the oil palm leaf tissues. Production of PHB requires the use of phbA (β-ketothiolase type A), phbB (acetoacetyl-CoA reductase) and phbC (PHB synt...

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Main Authors: Mat Yunus, Abdul Masani, Ghulam Kadir, Ahmad Parveez, Abang Masli, Dayang Izawati, Chan, Pek Lan, Abdullah, Siti Nor Akmar
Format: Article
Language:English
English
Published: 2009
Online Access:http://psasir.upm.edu.my/id/eprint/5380/1/Construction%20of%20PHB%20and%20PHBV%20multiple.pdf
http://psasir.upm.edu.my/id/eprint/5380/
http://dx.doi.org/10.1016/j.plasmid.2009.08.002
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spelling my.upm.eprints.53802015-10-22T06:09:45Z http://psasir.upm.edu.my/id/eprint/5380/ Construction of PHB and PHBV multiple-gene vectors driven by an oil palm leaf-specific promoter Mat Yunus, Abdul Masani Ghulam Kadir, Ahmad Parveez Abang Masli, Dayang Izawati Chan, Pek Lan Abdullah, Siti Nor Akmar One of the targets in oil palm genetic engineering programme is the production of polyhydroxybutyrate (PHB) and polyhydroxybutyrate-co-valerate (PHBV) in the oil palm leaf tissues. Production of PHB requires the use of phbA (β-ketothiolase type A), phbB (acetoacetyl-CoA reductase) and phbC (PHB synthase) genes of Ralstonia eutropha, whereas bktB (β-ketothiolase type B), phbB, phbC genes of R. eutropha and tdcB (threonine dehydratase) gene of Escherichia coli were used for PHBV production. Each of these genes was fused with a transit peptide (Tp) of oil palm acyl-carrier-protein (ACP) gene, driven by an oil palm leaf-specific promoter (LSP1) to genetically engineer the PHB/PHBV pathway to the plastids of the leaf tissues. In total, four transformation vectors, designated pLSP15 (PHB) and pLSP20 (PHBV), and pLSP13 (PHB) and pLSP23 (PHBV), were constructed for transformation in Arabidopsis thaliana and oil palm, respectively. The phosphinothricin acetyltransferase gene (bar) driven by CaMV35S promoter in pLSP15 and pLSP20, and ubiquitin promoter in pLSP13 and pLSP23 were used as the plant selectable markers. Matrix attachment region of tobacco (RB7MAR) was also included in the vectors to stabilize the transgene expression and to minimize silencing due to positional effect. Restriction digestion, PCR amplification and/or sequencing were carried out to ensure sequence integrity and orientation. 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/5380/1/Construction%20of%20PHB%20and%20PHBV%20multiple.pdf Mat Yunus, Abdul Masani and Ghulam Kadir, Ahmad Parveez and Abang Masli, Dayang Izawati and Chan, Pek Lan and Abdullah, Siti Nor Akmar (2009) Construction of PHB and PHBV multiple-gene vectors driven by an oil palm leaf-specific promoter. Plasmid, 62 (3). pp. 191-200. ISSN 0147-619X http://dx.doi.org/10.1016/j.plasmid.2009.08.002 10.1016/j.plasmid.2009.08.002 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description One of the targets in oil palm genetic engineering programme is the production of polyhydroxybutyrate (PHB) and polyhydroxybutyrate-co-valerate (PHBV) in the oil palm leaf tissues. Production of PHB requires the use of phbA (β-ketothiolase type A), phbB (acetoacetyl-CoA reductase) and phbC (PHB synthase) genes of Ralstonia eutropha, whereas bktB (β-ketothiolase type B), phbB, phbC genes of R. eutropha and tdcB (threonine dehydratase) gene of Escherichia coli were used for PHBV production. Each of these genes was fused with a transit peptide (Tp) of oil palm acyl-carrier-protein (ACP) gene, driven by an oil palm leaf-specific promoter (LSP1) to genetically engineer the PHB/PHBV pathway to the plastids of the leaf tissues. In total, four transformation vectors, designated pLSP15 (PHB) and pLSP20 (PHBV), and pLSP13 (PHB) and pLSP23 (PHBV), were constructed for transformation in Arabidopsis thaliana and oil palm, respectively. The phosphinothricin acetyltransferase gene (bar) driven by CaMV35S promoter in pLSP15 and pLSP20, and ubiquitin promoter in pLSP13 and pLSP23 were used as the plant selectable markers. Matrix attachment region of tobacco (RB7MAR) was also included in the vectors to stabilize the transgene expression and to minimize silencing due to positional effect. Restriction digestion, PCR amplification and/or sequencing were carried out to ensure sequence integrity and orientation.
format Article
author Mat Yunus, Abdul Masani
Ghulam Kadir, Ahmad Parveez
Abang Masli, Dayang Izawati
Chan, Pek Lan
Abdullah, Siti Nor Akmar
spellingShingle Mat Yunus, Abdul Masani
Ghulam Kadir, Ahmad Parveez
Abang Masli, Dayang Izawati
Chan, Pek Lan
Abdullah, Siti Nor Akmar
Construction of PHB and PHBV multiple-gene vectors driven by an oil palm leaf-specific promoter
author_facet Mat Yunus, Abdul Masani
Ghulam Kadir, Ahmad Parveez
Abang Masli, Dayang Izawati
Chan, Pek Lan
Abdullah, Siti Nor Akmar
author_sort Mat Yunus, Abdul Masani
title Construction of PHB and PHBV multiple-gene vectors driven by an oil palm leaf-specific promoter
title_short Construction of PHB and PHBV multiple-gene vectors driven by an oil palm leaf-specific promoter
title_full Construction of PHB and PHBV multiple-gene vectors driven by an oil palm leaf-specific promoter
title_fullStr Construction of PHB and PHBV multiple-gene vectors driven by an oil palm leaf-specific promoter
title_full_unstemmed Construction of PHB and PHBV multiple-gene vectors driven by an oil palm leaf-specific promoter
title_sort construction of phb and phbv multiple-gene vectors driven by an oil palm leaf-specific promoter
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/5380/1/Construction%20of%20PHB%20and%20PHBV%20multiple.pdf
http://psasir.upm.edu.my/id/eprint/5380/
http://dx.doi.org/10.1016/j.plasmid.2009.08.002
_version_ 1643823174246203392
score 13.211869

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