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Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production

Large numbers of glycerol-consuming bacteria are present in nature; hence bioconversion of glycerol into biofuel which is bioethanol is one of the interests. The effective screening procedure is needed to screen and isolate broad ranges of bacteria from environment. The screening method was modified...

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Main Authors: Suhaimi, Sheril Norliana, Adnan, Nur Amelia Azreen, Phang, Lai Yee, Abd Aziz, Suraini, Maeda, Toshinari
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2016
Online Access:http://psasir.upm.edu.my/id/eprint/53726/1/Rapid%20screening%20method%20for%20isolation%20of%20glycerol-consuming%20bacteria%20for%20ethanol%20production.pdf
http://psasir.upm.edu.my/id/eprint/53726/
http://www.ukm.my/jsm/english_journals/vol45num5_2016/vol45num5_2016p811-815.html
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spelling my.upm.eprints.537262018-01-11T04:38:22Z http://psasir.upm.edu.my/id/eprint/53726/ Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production Suhaimi, Sheril Norliana Adnan, Nur Amelia Azreen Phang, Lai Yee Abd Aziz, Suraini Maeda, Toshinari Large numbers of glycerol-consuming bacteria are present in nature; hence bioconversion of glycerol into biofuel which is bioethanol is one of the interests. The effective screening procedure is needed to screen and isolate broad ranges of bacteria from environment. The screening method was modified based on enzymatic oxidation of ethanol, which is correlated to reduction of 2,6-dichlorophenol-indophenol dye that resulted in the formation of yellow zone. Approximately 300 colonies were able to grow on minimal media using glycerol as sole carbon. Only about 70 isolates showed positive result when using the modified ethanol production assay after pre-screening stage. The formation of decolourized zone was apparent using modified assay containing 5 mL/L of 0.05M 2,6-dichlorophenol-indophenol, 10 mL of reaction mixture and 500 μl/L of enzyme, respectively. The ethanol production capability of the isolates was further proven by anaerobic fermentation as a quantitative method. This modified method is applicable in screening for ethanol producer from glycerol as carbon source allows rapid and more bacteria can be screened. Universiti Kebangsaan Malaysia 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53726/1/Rapid%20screening%20method%20for%20isolation%20of%20glycerol-consuming%20bacteria%20for%20ethanol%20production.pdf Suhaimi, Sheril Norliana and Adnan, Nur Amelia Azreen and Phang, Lai Yee and Abd Aziz, Suraini and Maeda, Toshinari (2016) Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production. Sains Malaysiana, 45 (5). pp. 811-815. ISSN 0026-6039 http://www.ukm.my/jsm/english_journals/vol45num5_2016/vol45num5_2016p811-815.html
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Large numbers of glycerol-consuming bacteria are present in nature; hence bioconversion of glycerol into biofuel which is bioethanol is one of the interests. The effective screening procedure is needed to screen and isolate broad ranges of bacteria from environment. The screening method was modified based on enzymatic oxidation of ethanol, which is correlated to reduction of 2,6-dichlorophenol-indophenol dye that resulted in the formation of yellow zone. Approximately 300 colonies were able to grow on minimal media using glycerol as sole carbon. Only about 70 isolates showed positive result when using the modified ethanol production assay after pre-screening stage. The formation of decolourized zone was apparent using modified assay containing 5 mL/L of 0.05M 2,6-dichlorophenol-indophenol, 10 mL of reaction mixture and 500 μl/L of enzyme, respectively. The ethanol production capability of the isolates was further proven by anaerobic fermentation as a quantitative method. This modified method is applicable in screening for ethanol producer from glycerol as carbon source allows rapid and more bacteria can be screened.
format Article
author Suhaimi, Sheril Norliana
Adnan, Nur Amelia Azreen
Phang, Lai Yee
Abd Aziz, Suraini
Maeda, Toshinari
spellingShingle Suhaimi, Sheril Norliana
Adnan, Nur Amelia Azreen
Phang, Lai Yee
Abd Aziz, Suraini
Maeda, Toshinari
Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
author_facet Suhaimi, Sheril Norliana
Adnan, Nur Amelia Azreen
Phang, Lai Yee
Abd Aziz, Suraini
Maeda, Toshinari
author_sort Suhaimi, Sheril Norliana
title Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
title_short Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
title_full Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
title_fullStr Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
title_full_unstemmed Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
title_sort rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
publisher Universiti Kebangsaan Malaysia
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/53726/1/Rapid%20screening%20method%20for%20isolation%20of%20glycerol-consuming%20bacteria%20for%20ethanol%20production.pdf
http://psasir.upm.edu.my/id/eprint/53726/
http://www.ukm.my/jsm/english_journals/vol45num5_2016/vol45num5_2016p811-815.html
_version_ 1643835471253471232
score 13.211869

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