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Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves

Melastoma malabathricum L., Melastomaceae, has been traditionally used to relieve diverse pain-related ailments. The objectives of the present study were to determine the antinociceptive activity of methanol extract of M. malabathricum leaves and to elucidate the possible mechanisms of antinocicepti...

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Main Authors: Jaios, Erman Shah, Abdul Rahman, Suzana, Ching, Siew Mooi, Abdul Kadir, Arifah, Mohd. Desa, Mohd. Nasir, Zakaria, Zainul Amirudin
Format: Article
Language:English
Published: Sociedade Brasileira de Farmacognosia 2016
Online Access:http://psasir.upm.edu.my/id/eprint/53608/1/Possible%20mechanisms%20of%20antinociception.pdf
http://psasir.upm.edu.my/id/eprint/53608/
http://www.sciencedirect.com/science/article/pii/S0102695X16300928
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spelling my.upm.eprints.536082017-11-15T04:33:48Z http://psasir.upm.edu.my/id/eprint/53608/ Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves Jaios, Erman Shah Abdul Rahman, Suzana Ching, Siew Mooi Abdul Kadir, Arifah Mohd. Desa, Mohd. Nasir Zakaria, Zainul Amirudin Melastoma malabathricum L., Melastomaceae, has been traditionally used to relieve diverse pain-related ailments. The objectives of the present study were to determine the antinociceptive activity of methanol extract of M. malabathricum leaves and to elucidate the possible mechanisms of antinociception involved using various rats’ models. The extract (100, 250, and 500 mg/kg) was administered orally 60 min prior to subjection to the respective test. The in vivo acetic acid-induced abdominal constriction, formalin-induced paw licking, and hot plate tests were used as the models of nociception to evaluate the extract antinociceptive activity. Further studies were carried out to determine the role of opioid and vanilloid receptors, glutamate system and nitric oxide/cyclic guanosine phosphate (NO/cGMP) pathway in modulating the extract antinociceptive activity. From the results obtained, M. malabathricum exhibited significant (p < 0.05) antinociceptive activity in all the chemical- and thermal-induced nociception models. Naloxone (5 mg/kg), a non-selective opioid antagonist, failed to significantly affect the antinociceptive activity of MEMM when assessed using the abdominal constriction-, hot plate- and formalin-induced paw licking-test. M. malabathricum also significantly (p < 0.05) reversed the nociceptive response in capsaicin- and glutamate-induced paw licking test. Furthermore, only l-arginine (a nitric oxide precursor) alone, but not, NG-nitro-l-arginine methyl esters (l-NAME; an inhibitor of NO synthase), methylene blue (MB; an inhibitor of cGMP), or their combination thereof, significantly (p < 0.05) block the antinociceptive activity of M. malabathricum. In conclusion, M. malabathricum exerted a non-opioid antinociceptive activity at the central and peripheral levels partly via the inhibition of vanilloid receptors and glutamatergic system, and activation of the NO-mediated/cGMP-independent pathway. Sociedade Brasileira de Farmacognosia 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53608/1/Possible%20mechanisms%20of%20antinociception.pdf Jaios, Erman Shah and Abdul Rahman, Suzana and Ching, Siew Mooi and Abdul Kadir, Arifah and Mohd. Desa, Mohd. Nasir and Zakaria, Zainul Amirudin (2016) Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves. Revista Brasileira de Farmacognosia, 26 (5). pp. 586-594. ISSN 0102-695X; ESSN: 1981-528X http://www.sciencedirect.com/science/article/pii/S0102695X16300928 10.1016/j.bjp.2016.01.011
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Melastoma malabathricum L., Melastomaceae, has been traditionally used to relieve diverse pain-related ailments. The objectives of the present study were to determine the antinociceptive activity of methanol extract of M. malabathricum leaves and to elucidate the possible mechanisms of antinociception involved using various rats’ models. The extract (100, 250, and 500 mg/kg) was administered orally 60 min prior to subjection to the respective test. The in vivo acetic acid-induced abdominal constriction, formalin-induced paw licking, and hot plate tests were used as the models of nociception to evaluate the extract antinociceptive activity. Further studies were carried out to determine the role of opioid and vanilloid receptors, glutamate system and nitric oxide/cyclic guanosine phosphate (NO/cGMP) pathway in modulating the extract antinociceptive activity. From the results obtained, M. malabathricum exhibited significant (p < 0.05) antinociceptive activity in all the chemical- and thermal-induced nociception models. Naloxone (5 mg/kg), a non-selective opioid antagonist, failed to significantly affect the antinociceptive activity of MEMM when assessed using the abdominal constriction-, hot plate- and formalin-induced paw licking-test. M. malabathricum also significantly (p < 0.05) reversed the nociceptive response in capsaicin- and glutamate-induced paw licking test. Furthermore, only l-arginine (a nitric oxide precursor) alone, but not, NG-nitro-l-arginine methyl esters (l-NAME; an inhibitor of NO synthase), methylene blue (MB; an inhibitor of cGMP), or their combination thereof, significantly (p < 0.05) block the antinociceptive activity of M. malabathricum. In conclusion, M. malabathricum exerted a non-opioid antinociceptive activity at the central and peripheral levels partly via the inhibition of vanilloid receptors and glutamatergic system, and activation of the NO-mediated/cGMP-independent pathway.
format Article
author Jaios, Erman Shah
Abdul Rahman, Suzana
Ching, Siew Mooi
Abdul Kadir, Arifah
Mohd. Desa, Mohd. Nasir
Zakaria, Zainul Amirudin
spellingShingle Jaios, Erman Shah
Abdul Rahman, Suzana
Ching, Siew Mooi
Abdul Kadir, Arifah
Mohd. Desa, Mohd. Nasir
Zakaria, Zainul Amirudin
Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves
author_facet Jaios, Erman Shah
Abdul Rahman, Suzana
Ching, Siew Mooi
Abdul Kadir, Arifah
Mohd. Desa, Mohd. Nasir
Zakaria, Zainul Amirudin
author_sort Jaios, Erman Shah
title Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves
title_short Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves
title_full Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves
title_fullStr Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves
title_full_unstemmed Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves
title_sort possible mechanisms of antinociception of methanol extract of melastoma malabathricum leaves
publisher Sociedade Brasileira de Farmacognosia
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/53608/1/Possible%20mechanisms%20of%20antinociception.pdf
http://psasir.upm.edu.my/id/eprint/53608/
http://www.sciencedirect.com/science/article/pii/S0102695X16300928
_version_ 1643835439231008768
score 13.211869

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