Cover Image

Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells

Cyphomandra betacea (C. betacea) belongs to the Solanaceae family. This study was aimed to evaluate the anti-proliferative of C.betacea crude extract against selected cancer cell lines (breast and liver cancer) and to identify the polyphenolics profile (phenolic acids and flavonoids) of C. betacea f...

Full description

Saved in:
Bibliographic Details
Main Authors: Abdul Mutalib, Maisarah, Ali, Faisal, Othman, Fauziah, Ramasamy, Rajesh, Rahmat, Asmah
Format: Article
Language:English
Published: SpringerOpen 2016
Online Access:http://psasir.upm.edu.my/id/eprint/53558/1/Phenolics%20profile%20and%20anti%20proliferative.pdf
http://psasir.upm.edu.my/id/eprint/53558/
https://search.proquest.com/openview/b9e215e8473816c412157166676fe138/1?pq-origsite=gscholar&cbl=2034663
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.53558
record_format eprints
spelling my.upm.eprints.535582017-11-08T02:01:32Z http://psasir.upm.edu.my/id/eprint/53558/ Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells Abdul Mutalib, Maisarah Ali, Faisal Othman, Fauziah Ramasamy, Rajesh Rahmat, Asmah Cyphomandra betacea (C. betacea) belongs to the Solanaceae family. This study was aimed to evaluate the anti-proliferative of C.betacea crude extract against selected cancer cell lines (breast and liver cancer) and to identify the polyphenolics profile (phenolic acids and flavonoids) of C. betacea fruits. Anti-proliferative effect of the extracts was examined using MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide) assay, followed by cell morphology analysis using acridine orange and propidium iodide double staining.The phenolics profile was characterized using high-performance liquid chromatography (HPLC). C. betacea extract showed a high cytotoxic effect against liver and breast cancer cell lines with the IC50 value of 30 and 80 µg/ml, respectively. Phenolics profiling showed that C. betacea extract has a wide variety of polyphenolic compounds which are the responsible substances underlying the cytotoxic activity. The microscopic examination analysed by acridine orange and propidium iodide double staining showed that viable cells of the liver and breast cells were well rounded, large and intact green nuclei compared with the treated cells that characterized by apoptotic features (shrunken nuclei with a less quantity of cytoplasm).The present study demonstrated that the anti-proliferative properties of C. betacea fruits were partially attributed to the rich phenolics content. It supports the hypothesis that C. betacea fruits have potential as an effective agent in cancer therapy. SpringerOpen 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53558/1/Phenolics%20profile%20and%20anti%20proliferative.pdf Abdul Mutalib, Maisarah and Ali, Faisal and Othman, Fauziah and Ramasamy, Rajesh and Rahmat, Asmah (2016) Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells. SpringerPlus, 5 (2105). pp. 1-13. ISSN 2193-1801 https://search.proquest.com/openview/b9e215e8473816c412157166676fe138/1?pq-origsite=gscholar&cbl=2034663 10.1186/s40064‑016‑3777‑x
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Cyphomandra betacea (C. betacea) belongs to the Solanaceae family. This study was aimed to evaluate the anti-proliferative of C.betacea crude extract against selected cancer cell lines (breast and liver cancer) and to identify the polyphenolics profile (phenolic acids and flavonoids) of C. betacea fruits. Anti-proliferative effect of the extracts was examined using MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide) assay, followed by cell morphology analysis using acridine orange and propidium iodide double staining.The phenolics profile was characterized using high-performance liquid chromatography (HPLC). C. betacea extract showed a high cytotoxic effect against liver and breast cancer cell lines with the IC50 value of 30 and 80 µg/ml, respectively. Phenolics profiling showed that C. betacea extract has a wide variety of polyphenolic compounds which are the responsible substances underlying the cytotoxic activity. The microscopic examination analysed by acridine orange and propidium iodide double staining showed that viable cells of the liver and breast cells were well rounded, large and intact green nuclei compared with the treated cells that characterized by apoptotic features (shrunken nuclei with a less quantity of cytoplasm).The present study demonstrated that the anti-proliferative properties of C. betacea fruits were partially attributed to the rich phenolics content. It supports the hypothesis that C. betacea fruits have potential as an effective agent in cancer therapy.
format Article
author Abdul Mutalib, Maisarah
Ali, Faisal
Othman, Fauziah
Ramasamy, Rajesh
Rahmat, Asmah
spellingShingle Abdul Mutalib, Maisarah
Ali, Faisal
Othman, Fauziah
Ramasamy, Rajesh
Rahmat, Asmah
Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells
author_facet Abdul Mutalib, Maisarah
Ali, Faisal
Othman, Fauziah
Ramasamy, Rajesh
Rahmat, Asmah
author_sort Abdul Mutalib, Maisarah
title Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells
title_short Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells
title_full Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells
title_fullStr Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells
title_full_unstemmed Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells
title_sort phenolics profile and anti-proliferative activity of cyphomandra betacea fruit in breast and liver cancer cells
publisher SpringerOpen
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/53558/1/Phenolics%20profile%20and%20anti%20proliferative.pdf
http://psasir.upm.edu.my/id/eprint/53558/
https://search.proquest.com/openview/b9e215e8473816c412157166676fe138/1?pq-origsite=gscholar&cbl=2034663
_version_ 1643835427042361344
score 13.211869

Similar Items