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Oil palm leaves and roots differ in physiological response, antioxidant enzyme activities and expression of stress-responsive genes upon exposure to drought stress

The seedling stage is a critical period for survival under drought stress. To identify biochemical and molecular drought response changes, oil palm seedlings were exposed to different levels of drought severity. Total chlorophyll, total soluble protein and total proline content were measured while e...

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Main Authors: Mohamad Azzeme, Azzreena, Abdullah, Siti Nor Akmar, Abdul Aziz, Maheran, Megat Wahab, Puteri Edaroyati
Format: Article
Language:English
Published: Springer 2016
Online Access:http://psasir.upm.edu.my/id/eprint/53419/1/Oil%20palm%20leaves%20and%20roots%20differ%20in%20physiological%20response%2C%20antioxidant%20enzyme%20activities%20and%20expression%20of%20stress-responsive%20genes%20upon%20exposure%20to%20drought%20stress.pdf
http://psasir.upm.edu.my/id/eprint/53419/
https://link.springer.com/article/10.1007/s11738-016-2073-2
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spelling my.upm.eprints.534192017-10-30T05:22:35Z http://psasir.upm.edu.my/id/eprint/53419/ Oil palm leaves and roots differ in physiological response, antioxidant enzyme activities and expression of stress-responsive genes upon exposure to drought stress Mohamad Azzeme, Azzreena Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Megat Wahab, Puteri Edaroyati The seedling stage is a critical period for survival under drought stress. To identify biochemical and molecular drought response changes, oil palm seedlings were exposed to different levels of drought severity. Total chlorophyll, total soluble protein and total proline content were measured while expression of stress responsive genes was quantified using qPCR. The diminishing total chlorophyll (chl) content and the ratio of chla to chlb (chla:chlb) were observed to be significant. The significant reduction of chla was closely related to photosystem II deficiency. Based on the effects of drought on chlorophyll content, the samples can be categorised into mild (7 days of water withholding; DWW), moderate (14 DWW) and severe (21, 28 and 35 DWW). Sample at 21 DWW was used to represent the severe stage. Genes encoding ethylene responsive binding protein, late embryogenesis abundant (LEA), dehydrin (DHN), cold-induced, heat shock protein 70 and metallothionein type 2 were differentially up-regulated in the leaves, while in the roots only LEA and DHN were up-regulated. The proline content increased gradually in both vegetative tissues, while the total soluble protein content was affected by increasing drought severity. The activity of catalase was highest in the roots at the severe drought stage, while guaicol peroxidase activity was shown to be highest in the leaves under mild drought. These findings provide new insights into stress tolerance mechanisms of oil palm seedlings and can be used to develop stress tolerant oil palm through classical breeding and genetic engineering. Springer 2016-02 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53419/1/Oil%20palm%20leaves%20and%20roots%20differ%20in%20physiological%20response%2C%20antioxidant%20enzyme%20activities%20and%20expression%20of%20stress-responsive%20genes%20upon%20exposure%20to%20drought%20stress.pdf Mohamad Azzeme, Azzreena and Abdullah, Siti Nor Akmar and Abdul Aziz, Maheran and Megat Wahab, Puteri Edaroyati (2016) Oil palm leaves and roots differ in physiological response, antioxidant enzyme activities and expression of stress-responsive genes upon exposure to drought stress. Acta Physiologiae Plantarum, 38 (52). pp. 1-12. ISSN 0137-5881; ESSN:1861-1664 https://link.springer.com/article/10.1007/s11738-016-2073-2 10.1007/s11738-016-2073-2
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The seedling stage is a critical period for survival under drought stress. To identify biochemical and molecular drought response changes, oil palm seedlings were exposed to different levels of drought severity. Total chlorophyll, total soluble protein and total proline content were measured while expression of stress responsive genes was quantified using qPCR. The diminishing total chlorophyll (chl) content and the ratio of chla to chlb (chla:chlb) were observed to be significant. The significant reduction of chla was closely related to photosystem II deficiency. Based on the effects of drought on chlorophyll content, the samples can be categorised into mild (7 days of water withholding; DWW), moderate (14 DWW) and severe (21, 28 and 35 DWW). Sample at 21 DWW was used to represent the severe stage. Genes encoding ethylene responsive binding protein, late embryogenesis abundant (LEA), dehydrin (DHN), cold-induced, heat shock protein 70 and metallothionein type 2 were differentially up-regulated in the leaves, while in the roots only LEA and DHN were up-regulated. The proline content increased gradually in both vegetative tissues, while the total soluble protein content was affected by increasing drought severity. The activity of catalase was highest in the roots at the severe drought stage, while guaicol peroxidase activity was shown to be highest in the leaves under mild drought. These findings provide new insights into stress tolerance mechanisms of oil palm seedlings and can be used to develop stress tolerant oil palm through classical breeding and genetic engineering.
format Article
author Mohamad Azzeme, Azzreena
Abdullah, Siti Nor Akmar
Abdul Aziz, Maheran
Megat Wahab, Puteri Edaroyati
spellingShingle Mohamad Azzeme, Azzreena
Abdullah, Siti Nor Akmar
Abdul Aziz, Maheran
Megat Wahab, Puteri Edaroyati
Oil palm leaves and roots differ in physiological response, antioxidant enzyme activities and expression of stress-responsive genes upon exposure to drought stress
author_facet Mohamad Azzeme, Azzreena
Abdullah, Siti Nor Akmar
Abdul Aziz, Maheran
Megat Wahab, Puteri Edaroyati
author_sort Mohamad Azzeme, Azzreena
title Oil palm leaves and roots differ in physiological response, antioxidant enzyme activities and expression of stress-responsive genes upon exposure to drought stress
title_short Oil palm leaves and roots differ in physiological response, antioxidant enzyme activities and expression of stress-responsive genes upon exposure to drought stress
title_full Oil palm leaves and roots differ in physiological response, antioxidant enzyme activities and expression of stress-responsive genes upon exposure to drought stress
title_fullStr Oil palm leaves and roots differ in physiological response, antioxidant enzyme activities and expression of stress-responsive genes upon exposure to drought stress
title_full_unstemmed Oil palm leaves and roots differ in physiological response, antioxidant enzyme activities and expression of stress-responsive genes upon exposure to drought stress
title_sort oil palm leaves and roots differ in physiological response, antioxidant enzyme activities and expression of stress-responsive genes upon exposure to drought stress
publisher Springer
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/53419/1/Oil%20palm%20leaves%20and%20roots%20differ%20in%20physiological%20response%2C%20antioxidant%20enzyme%20activities%20and%20expression%20of%20stress-responsive%20genes%20upon%20exposure%20to%20drought%20stress.pdf
http://psasir.upm.edu.my/id/eprint/53419/
https://link.springer.com/article/10.1007/s11738-016-2073-2
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score 13.211869

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