Cover Image

Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway

CBF/DREB1 is a group of transcription factors that are mainly involved in abiotic stress tolerance in plants. They belong to the AP2/ERF superfamily of plant-specific transcription factors. A gene encoding a new member of this group was isolated from ripening oil palm fruit and designated as EgCBF3....

Full description

Saved in:
Bibliographic Details
Main Authors: Ebrahimi, Mortaza, Abdullah, Siti Nor Akmar, Abdul Aziz, Maheran, Namasivayam, Parameswari
Format: Article
Language:English
Published: Elsevier 2016
Online Access:http://psasir.upm.edu.my/id/eprint/53418/1/Oil%20palm%20EgCBF3%20conferred%20stress%20tolerance%20in%20transgenic%20tomato%20plants%20through%20modulation%20of%20the%20ethylene%20signaling%20pathway.pdf
http://psasir.upm.edu.my/id/eprint/53418/
http://www.sciencedirect.com/science/article/pii/S0176161716301213
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.53418
record_format eprints
spelling my.upm.eprints.534182017-10-30T05:07:19Z http://psasir.upm.edu.my/id/eprint/53418/ Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway Ebrahimi, Mortaza Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Namasivayam, Parameswari CBF/DREB1 is a group of transcription factors that are mainly involved in abiotic stress tolerance in plants. They belong to the AP2/ERF superfamily of plant-specific transcription factors. A gene encoding a new member of this group was isolated from ripening oil palm fruit and designated as EgCBF3. The oil palm fruit demonstrates the characteristics of a climacteric fruit like tomato, in which ethylene has a major impact on the ripening process. A transgenic approach was used for functional characterization of the EgCBF3, using tomato as the model plant. The effects of ectopic expression of EgCBF3 were analyzed based on expression profiling of the ethylene biosynthesis-related genes, anti-freeze proteins (AFPs), abiotic stress tolerance and plant growth and development. The EgCBF3 tomatoes demonstrated altered phenotypes compared to the wild type tomatoes. Delayed leaf senescence and flowering, increased chlorophyll content and abnormal flowering were the consequences of overexpression of EgCBF3 in the transgenic tomatoes. The EgCBF3 tomatoes demonstrated enhanced abiotic stress tolerance under in vitro conditions. Further, transcript levels of ethylene biosynthesis-related genes, including three SlACSs and two SlACOs, were altered in the transgenic plants’ leaves and roots compared to that in the wild type tomato plant. Among the eight AFPs studied in the wounded leaves of the EgCBF3 tomato plants, transcript levels of SlOSM-L, SlNP24, SlPR5L and SlTSRF1 decreased, while expression of the other four, SlCHI3, SlPR1, SlPR-P2 and SlLAP2, were up-regulated. These findings indicate the possible functions of EgCBF3 in plant growth and development as a regulator of ethylene biosynthesis-related and AFP genes, and as a stimulator of abiotic stress tolerance. Elsevier 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53418/1/Oil%20palm%20EgCBF3%20conferred%20stress%20tolerance%20in%20transgenic%20tomato%20plants%20through%20modulation%20of%20the%20ethylene%20signaling%20pathway.pdf Ebrahimi, Mortaza and Abdullah, Siti Nor Akmar and Abdul Aziz, Maheran and Namasivayam, Parameswari (2016) Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway. Journal of Plant Physiology, 202. pp. 107-120. ISSN 0176-1617; ESSN: 1618-1328 http://www.sciencedirect.com/science/article/pii/S0176161716301213 10.1016/j.jplph.2016.07.001
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description CBF/DREB1 is a group of transcription factors that are mainly involved in abiotic stress tolerance in plants. They belong to the AP2/ERF superfamily of plant-specific transcription factors. A gene encoding a new member of this group was isolated from ripening oil palm fruit and designated as EgCBF3. The oil palm fruit demonstrates the characteristics of a climacteric fruit like tomato, in which ethylene has a major impact on the ripening process. A transgenic approach was used for functional characterization of the EgCBF3, using tomato as the model plant. The effects of ectopic expression of EgCBF3 were analyzed based on expression profiling of the ethylene biosynthesis-related genes, anti-freeze proteins (AFPs), abiotic stress tolerance and plant growth and development. The EgCBF3 tomatoes demonstrated altered phenotypes compared to the wild type tomatoes. Delayed leaf senescence and flowering, increased chlorophyll content and abnormal flowering were the consequences of overexpression of EgCBF3 in the transgenic tomatoes. The EgCBF3 tomatoes demonstrated enhanced abiotic stress tolerance under in vitro conditions. Further, transcript levels of ethylene biosynthesis-related genes, including three SlACSs and two SlACOs, were altered in the transgenic plants’ leaves and roots compared to that in the wild type tomato plant. Among the eight AFPs studied in the wounded leaves of the EgCBF3 tomato plants, transcript levels of SlOSM-L, SlNP24, SlPR5L and SlTSRF1 decreased, while expression of the other four, SlCHI3, SlPR1, SlPR-P2 and SlLAP2, were up-regulated. These findings indicate the possible functions of EgCBF3 in plant growth and development as a regulator of ethylene biosynthesis-related and AFP genes, and as a stimulator of abiotic stress tolerance.
format Article
author Ebrahimi, Mortaza
Abdullah, Siti Nor Akmar
Abdul Aziz, Maheran
Namasivayam, Parameswari
spellingShingle Ebrahimi, Mortaza
Abdullah, Siti Nor Akmar
Abdul Aziz, Maheran
Namasivayam, Parameswari
Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway
author_facet Ebrahimi, Mortaza
Abdullah, Siti Nor Akmar
Abdul Aziz, Maheran
Namasivayam, Parameswari
author_sort Ebrahimi, Mortaza
title Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway
title_short Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway
title_full Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway
title_fullStr Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway
title_full_unstemmed Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway
title_sort oil palm egcbf3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway
publisher Elsevier
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/53418/1/Oil%20palm%20EgCBF3%20conferred%20stress%20tolerance%20in%20transgenic%20tomato%20plants%20through%20modulation%20of%20the%20ethylene%20signaling%20pathway.pdf
http://psasir.upm.edu.my/id/eprint/53418/
http://www.sciencedirect.com/science/article/pii/S0176161716301213
_version_ 1643835390248878080
score 13.211869

Similar Items