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Zerumbone-loaded nanostructured lipid carrier induces apoptosis in human colorectal adenocarcinoma (Caco2) cell line

The incorporation of zerumbone (Zer) into nanostructured lipid carrier (NLC) is hypothesized to increase the efficacy of the drug. Nanostructured lipid carrier has sustained-drug release characteristics and is able to improve the solubility and bioavailability of the lipophilic drug. In this study,...

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Main Authors: Nathaniel, Christian, Yi, Lin Elaine-Lee, Beh, Chaw Yee, Chee, Wun How, Hip, Seng Yim, Rasadee, Abdullah, Hui, Suan Ng
Format: Article
Language:English
Published: American Scientific Publishers 2016
Online Access:http://psasir.upm.edu.my/id/eprint/53269/1/123.pdf
http://psasir.upm.edu.my/id/eprint/53269/
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spelling my.upm.eprints.532692017-07-27T09:04:37Z http://psasir.upm.edu.my/id/eprint/53269/ Zerumbone-loaded nanostructured lipid carrier induces apoptosis in human colorectal adenocarcinoma (Caco2) cell line Nathaniel, Christian Yi, Lin Elaine-Lee Beh, Chaw Yee Chee, Wun How Hip, Seng Yim Rasadee, Abdullah Hui, Suan Ng The incorporation of zerumbone (Zer) into nanostructured lipid carrier (NLC) is hypothesized to increase the efficacy of the drug. Nanostructured lipid carrier has sustained-drug release characteristics and is able to improve the solubility and bioavailability of the lipophilic drug. In this study, the anti-cancer effect of Zer was tested on human colorectal adenocarcinoma (Caco-2) cell line. The effect of Zer, zerumbone-loaded nanostructured lipid carrier (Zer-NLC) and NLC on the Caco-2 cell viability were determined using the MTT assay. The treatment concentration ranges from 0 to 120 μM at four different time intervals (i.e., 0 h, 24 hrs, 48 hrs and 72 hrs) were evaluated. At 24 hrs, the half-growth inhibitory concentration (GI50) of Zer-NLC (i.e., 4.25 μM) is lower than that of Zer (i.e., 23.75 μM). However, Zer outperformed the Zer-NLC at the subsequent time points. Similar trend was observed in other parameters including the cytostatic concentration (CC) and half-lethal concentration 50 (LC50). Phase contrast imaging and AO/PI fluorescence staining were performed at the CC and LC50 values. The morphological changes and the apoptosis features could be seen in cells treated with Zer and Zer-NLC while cells treated with NLC showed minor morphological changes. The cells treated with Zer-NLC demonstrated a slightly slower progression of apoptosis, which could be due to the controlled release of Zer from the NLC matrix. It was concluded that the incorporation of Zer into NLC did not compromise the potency and efficacy of the drug. American Scientific Publishers 2016-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53269/1/123.pdf Nathaniel, Christian and Yi, Lin Elaine-Lee and Beh, Chaw Yee and Chee, Wun How and Hip, Seng Yim and Rasadee, Abdullah and Hui, Suan Ng (2016) Zerumbone-loaded nanostructured lipid carrier induces apoptosis in human colorectal adenocarcinoma (Caco2) cell line. Nanoscience and Nanotechnology Letters, 8 (4). pp. 294-302. ISSN 1941-4900; ESSN: 1941-4919 10.1166/nnl.2016.2136
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The incorporation of zerumbone (Zer) into nanostructured lipid carrier (NLC) is hypothesized to increase the efficacy of the drug. Nanostructured lipid carrier has sustained-drug release characteristics and is able to improve the solubility and bioavailability of the lipophilic drug. In this study, the anti-cancer effect of Zer was tested on human colorectal adenocarcinoma (Caco-2) cell line. The effect of Zer, zerumbone-loaded nanostructured lipid carrier (Zer-NLC) and NLC on the Caco-2 cell viability were determined using the MTT assay. The treatment concentration ranges from 0 to 120 μM at four different time intervals (i.e., 0 h, 24 hrs, 48 hrs and 72 hrs) were evaluated. At 24 hrs, the half-growth inhibitory concentration (GI50) of Zer-NLC (i.e., 4.25 μM) is lower than that of Zer (i.e., 23.75 μM). However, Zer outperformed the Zer-NLC at the subsequent time points. Similar trend was observed in other parameters including the cytostatic concentration (CC) and half-lethal concentration 50 (LC50). Phase contrast imaging and AO/PI fluorescence staining were performed at the CC and LC50 values. The morphological changes and the apoptosis features could be seen in cells treated with Zer and Zer-NLC while cells treated with NLC showed minor morphological changes. The cells treated with Zer-NLC demonstrated a slightly slower progression of apoptosis, which could be due to the controlled release of Zer from the NLC matrix. It was concluded that the incorporation of Zer into NLC did not compromise the potency and efficacy of the drug.
format Article
author Nathaniel, Christian
Yi, Lin Elaine-Lee
Beh, Chaw Yee
Chee, Wun How
Hip, Seng Yim
Rasadee, Abdullah
Hui, Suan Ng
spellingShingle Nathaniel, Christian
Yi, Lin Elaine-Lee
Beh, Chaw Yee
Chee, Wun How
Hip, Seng Yim
Rasadee, Abdullah
Hui, Suan Ng
Zerumbone-loaded nanostructured lipid carrier induces apoptosis in human colorectal adenocarcinoma (Caco2) cell line
author_facet Nathaniel, Christian
Yi, Lin Elaine-Lee
Beh, Chaw Yee
Chee, Wun How
Hip, Seng Yim
Rasadee, Abdullah
Hui, Suan Ng
author_sort Nathaniel, Christian
title Zerumbone-loaded nanostructured lipid carrier induces apoptosis in human colorectal adenocarcinoma (Caco2) cell line
title_short Zerumbone-loaded nanostructured lipid carrier induces apoptosis in human colorectal adenocarcinoma (Caco2) cell line
title_full Zerumbone-loaded nanostructured lipid carrier induces apoptosis in human colorectal adenocarcinoma (Caco2) cell line
title_fullStr Zerumbone-loaded nanostructured lipid carrier induces apoptosis in human colorectal adenocarcinoma (Caco2) cell line
title_full_unstemmed Zerumbone-loaded nanostructured lipid carrier induces apoptosis in human colorectal adenocarcinoma (Caco2) cell line
title_sort zerumbone-loaded nanostructured lipid carrier induces apoptosis in human colorectal adenocarcinoma (caco2) cell line
publisher American Scientific Publishers
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/53269/1/123.pdf
http://psasir.upm.edu.my/id/eprint/53269/
_version_ 1643835357625581568
score 13.211869

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