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Transducer-like enhancer of split 1 (TLE1) expression as a diagnostic immunohistochemical marker for synovial sarcoma and its association with morphological features

Synovial sarcoma (SS) is a malignant soft tissue tumour of uncertain histogenesis which is defined by the translocation t(X;18) that produces the fusion oncogenes SYT-SSX. The emergence of transducer-like enhancer of split 1 (TLE1) as a new immunohistochemical (IHC) marker for SS has offered an alte...

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Main Authors: Bakrin, Ikmal Hisyam, Hussain, Faezahtul Arbaeyah, Tuan Sharif, Sharifah Emilia
Format: Article
Language:English
Published: Malaysian Society of Pathologists 2016
Online Access:http://psasir.upm.edu.my/id/eprint/53155/1/Transducer-like%20enhancer%20of%20split%201%20%28TLE1%29%20expression%20as%20a%20diagnostic%20immunohistochemical%20marker%20for%20synovial%20sarcoma%20and%20its%20association%20with%20morphological%20features.pdf
http://psasir.upm.edu.my/id/eprint/53155/
http://www.mjpath.org.my/2016/v38n2/TLE1.pdf
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spelling my.upm.eprints.531552017-10-30T10:53:12Z http://psasir.upm.edu.my/id/eprint/53155/ Transducer-like enhancer of split 1 (TLE1) expression as a diagnostic immunohistochemical marker for synovial sarcoma and its association with morphological features Bakrin, Ikmal Hisyam Hussain, Faezahtul Arbaeyah Tuan Sharif, Sharifah Emilia Synovial sarcoma (SS) is a malignant soft tissue tumour of uncertain histogenesis which is defined by the translocation t(X;18) that produces the fusion oncogenes SYT-SSX. The emergence of transducer-like enhancer of split 1 (TLE1) as a new immunohistochemical (IHC) marker for SS has offered an alternative to pathologists in differentiating SS from other histological mimics, especially in the setting of limited molecular facilities. We investigated the utility of IHC TLE1 expression against histomorphological features and other IHC markers in SS and non-SS tumours. Twenty-six cases of histologically diagnosed SS and 7 non-SS (for which SS was in the differential diagnosis) were subjected to TLE1 IHC staining, which was graded from 0 to 3+. Of the 26 SS cases, 12 each were biphasic and monophasic types and 2 were poorly-differentiated. TLE1 was expressed in 22/26 (84.6%) SS cases, of which 11/12 (91.7%) were biphasic, 10/12 (83.3%) monophasic and 1/2 (50%) poorly-differentiated tumours. Two of 7 (28.6%) non-SS cases were positive for TLE1. Immunopositivity of SS and non-SS cases for EMA were 20/26 (76.9%) and 2/7 (28.6%) respectively and for CK7 were 7/26 (26.9%) and 0/7 (0%) respectively. All cases were negative for CD34. Consistent histomorphological features for SS included mild nuclear pleomorphism, alternating tumour cellularity, fascicular growth pattern and thick ropy stromal collagen. In conclusion, TLE1 is not a stand-alone diagnostic IHC marker for SS. However, in the absence of molecular studies, it can contribute added diagnostic value in combination with morphological evaluation and other IHC markers such as EMA and CD34. Malaysian Society of Pathologists 2016-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53155/1/Transducer-like%20enhancer%20of%20split%201%20%28TLE1%29%20expression%20as%20a%20diagnostic%20immunohistochemical%20marker%20for%20synovial%20sarcoma%20and%20its%20association%20with%20morphological%20features.pdf Bakrin, Ikmal Hisyam and Hussain, Faezahtul Arbaeyah and Tuan Sharif, Sharifah Emilia (2016) Transducer-like enhancer of split 1 (TLE1) expression as a diagnostic immunohistochemical marker for synovial sarcoma and its association with morphological features. Malaysian Journal of Pathology, 38 (2). pp. 117-122. ISSN 0126-8635 http://www.mjpath.org.my/2016/v38n2/TLE1.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Synovial sarcoma (SS) is a malignant soft tissue tumour of uncertain histogenesis which is defined by the translocation t(X;18) that produces the fusion oncogenes SYT-SSX. The emergence of transducer-like enhancer of split 1 (TLE1) as a new immunohistochemical (IHC) marker for SS has offered an alternative to pathologists in differentiating SS from other histological mimics, especially in the setting of limited molecular facilities. We investigated the utility of IHC TLE1 expression against histomorphological features and other IHC markers in SS and non-SS tumours. Twenty-six cases of histologically diagnosed SS and 7 non-SS (for which SS was in the differential diagnosis) were subjected to TLE1 IHC staining, which was graded from 0 to 3+. Of the 26 SS cases, 12 each were biphasic and monophasic types and 2 were poorly-differentiated. TLE1 was expressed in 22/26 (84.6%) SS cases, of which 11/12 (91.7%) were biphasic, 10/12 (83.3%) monophasic and 1/2 (50%) poorly-differentiated tumours. Two of 7 (28.6%) non-SS cases were positive for TLE1. Immunopositivity of SS and non-SS cases for EMA were 20/26 (76.9%) and 2/7 (28.6%) respectively and for CK7 were 7/26 (26.9%) and 0/7 (0%) respectively. All cases were negative for CD34. Consistent histomorphological features for SS included mild nuclear pleomorphism, alternating tumour cellularity, fascicular growth pattern and thick ropy stromal collagen. In conclusion, TLE1 is not a stand-alone diagnostic IHC marker for SS. However, in the absence of molecular studies, it can contribute added diagnostic value in combination with morphological evaluation and other IHC markers such as EMA and CD34.
format Article
author Bakrin, Ikmal Hisyam
Hussain, Faezahtul Arbaeyah
Tuan Sharif, Sharifah Emilia
spellingShingle Bakrin, Ikmal Hisyam
Hussain, Faezahtul Arbaeyah
Tuan Sharif, Sharifah Emilia
Transducer-like enhancer of split 1 (TLE1) expression as a diagnostic immunohistochemical marker for synovial sarcoma and its association with morphological features
author_facet Bakrin, Ikmal Hisyam
Hussain, Faezahtul Arbaeyah
Tuan Sharif, Sharifah Emilia
author_sort Bakrin, Ikmal Hisyam
title Transducer-like enhancer of split 1 (TLE1) expression as a diagnostic immunohistochemical marker for synovial sarcoma and its association with morphological features
title_short Transducer-like enhancer of split 1 (TLE1) expression as a diagnostic immunohistochemical marker for synovial sarcoma and its association with morphological features
title_full Transducer-like enhancer of split 1 (TLE1) expression as a diagnostic immunohistochemical marker for synovial sarcoma and its association with morphological features
title_fullStr Transducer-like enhancer of split 1 (TLE1) expression as a diagnostic immunohistochemical marker for synovial sarcoma and its association with morphological features
title_full_unstemmed Transducer-like enhancer of split 1 (TLE1) expression as a diagnostic immunohistochemical marker for synovial sarcoma and its association with morphological features
title_sort transducer-like enhancer of split 1 (tle1) expression as a diagnostic immunohistochemical marker for synovial sarcoma and its association with morphological features
publisher Malaysian Society of Pathologists
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/53155/1/Transducer-like%20enhancer%20of%20split%201%20%28TLE1%29%20expression%20as%20a%20diagnostic%20immunohistochemical%20marker%20for%20synovial%20sarcoma%20and%20its%20association%20with%20morphological%20features.pdf
http://psasir.upm.edu.my/id/eprint/53155/
http://www.mjpath.org.my/2016/v38n2/TLE1.pdf
_version_ 1643835333235703808
score 13.211869

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