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The inhibitory activity of cocoa phenolic extract against pro-inflammatory mediators secretion induced by lipopolysaccharide in RAW 264.7 cells

Cocoa is a rich source of polyphenols that has been traditionally used as the treatment of several types of inflammation related disease. The response to inflammation comprises the consecutive release of mediators and the enlistment of circulating leukocytes, such as macrophages. Currently, Cocoa-de...

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Main Authors: Ranneh, Yazan, Ali, Faisal, Al-Qubaisi, Mothanna, Norhaizan, Mohd Esa, Ismail, Amin
Format: Article
Language:English
Published: SpringerOpen 2016
Online Access:http://psasir.upm.edu.my/id/eprint/53037/1/The%20inhibitory%20activity%20of%20cocoa%20phenolic%20extract%20against%20pro-inflammatory%20mediators%20secretion%20induced%20by%20lipopolysaccharide%20in%20RAW%20264.7%20cells.pdf
http://psasir.upm.edu.my/id/eprint/53037/
http://www.springerplus.com
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spelling my.upm.eprints.530372017-11-17T01:56:46Z http://psasir.upm.edu.my/id/eprint/53037/ The inhibitory activity of cocoa phenolic extract against pro-inflammatory mediators secretion induced by lipopolysaccharide in RAW 264.7 cells Ranneh, Yazan Ali, Faisal Al-Qubaisi, Mothanna Norhaizan, Mohd Esa Ismail, Amin Cocoa is a rich source of polyphenols that has been traditionally used as the treatment of several types of inflammation related disease. The response to inflammation comprises the consecutive release of mediators and the enlistment of circulating leukocytes, such as macrophages. Currently, Cocoa-derived polyphenolics have shown anti-inflammatory effects in vivo, but the therapeutic benefits in vitro remain unclear. Therefore, in this study, the effect of cocoa polyphenolic extract (CPE) on RAW 264.7 macrophage cells sensitized by lipopolysaccharide as in vitro inflammatory model was investigated. The anti-inflammatory activity of CPE was assessed by measuring its ability to inhibit the pro-inflammatory enzyme 5-lipoxygenase (5-LOX) and the pro-inflammatory mediators prostaglandin E2 (PGE2), reactive oxygen species (ROS), nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α). The results show that CPE significantly inhibits 5-LOX activity (p < 0.01). In addition, CPE dose-dependently suppressed the production of PGE2, ROS, NO and TNF-α in RAW 264.7 cells. These data suggest that CPE may be used for the treatment of inflammation and it’s related-diseases. SpringerOpen 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53037/1/The%20inhibitory%20activity%20of%20cocoa%20phenolic%20extract%20against%20pro-inflammatory%20mediators%20secretion%20induced%20by%20lipopolysaccharide%20in%20RAW%20264.7%20cells.pdf Ranneh, Yazan and Ali, Faisal and Al-Qubaisi, Mothanna and Norhaizan, Mohd Esa and Ismail, Amin (2016) The inhibitory activity of cocoa phenolic extract against pro-inflammatory mediators secretion induced by lipopolysaccharide in RAW 264.7 cells. SpringerPlus, 5 (547). pp. 1-10. ISSN 2193-1801 http://www.springerplus.com 10.1186/s40064-016-2138-0
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Cocoa is a rich source of polyphenols that has been traditionally used as the treatment of several types of inflammation related disease. The response to inflammation comprises the consecutive release of mediators and the enlistment of circulating leukocytes, such as macrophages. Currently, Cocoa-derived polyphenolics have shown anti-inflammatory effects in vivo, but the therapeutic benefits in vitro remain unclear. Therefore, in this study, the effect of cocoa polyphenolic extract (CPE) on RAW 264.7 macrophage cells sensitized by lipopolysaccharide as in vitro inflammatory model was investigated. The anti-inflammatory activity of CPE was assessed by measuring its ability to inhibit the pro-inflammatory enzyme 5-lipoxygenase (5-LOX) and the pro-inflammatory mediators prostaglandin E2 (PGE2), reactive oxygen species (ROS), nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α). The results show that CPE significantly inhibits 5-LOX activity (p < 0.01). In addition, CPE dose-dependently suppressed the production of PGE2, ROS, NO and TNF-α in RAW 264.7 cells. These data suggest that CPE may be used for the treatment of inflammation and it’s related-diseases.
format Article
author Ranneh, Yazan
Ali, Faisal
Al-Qubaisi, Mothanna
Norhaizan, Mohd Esa
Ismail, Amin
spellingShingle Ranneh, Yazan
Ali, Faisal
Al-Qubaisi, Mothanna
Norhaizan, Mohd Esa
Ismail, Amin
The inhibitory activity of cocoa phenolic extract against pro-inflammatory mediators secretion induced by lipopolysaccharide in RAW 264.7 cells
author_facet Ranneh, Yazan
Ali, Faisal
Al-Qubaisi, Mothanna
Norhaizan, Mohd Esa
Ismail, Amin
author_sort Ranneh, Yazan
title The inhibitory activity of cocoa phenolic extract against pro-inflammatory mediators secretion induced by lipopolysaccharide in RAW 264.7 cells
title_short The inhibitory activity of cocoa phenolic extract against pro-inflammatory mediators secretion induced by lipopolysaccharide in RAW 264.7 cells
title_full The inhibitory activity of cocoa phenolic extract against pro-inflammatory mediators secretion induced by lipopolysaccharide in RAW 264.7 cells
title_fullStr The inhibitory activity of cocoa phenolic extract against pro-inflammatory mediators secretion induced by lipopolysaccharide in RAW 264.7 cells
title_full_unstemmed The inhibitory activity of cocoa phenolic extract against pro-inflammatory mediators secretion induced by lipopolysaccharide in RAW 264.7 cells
title_sort inhibitory activity of cocoa phenolic extract against pro-inflammatory mediators secretion induced by lipopolysaccharide in raw 264.7 cells
publisher SpringerOpen
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/53037/1/The%20inhibitory%20activity%20of%20cocoa%20phenolic%20extract%20against%20pro-inflammatory%20mediators%20secretion%20induced%20by%20lipopolysaccharide%20in%20RAW%20264.7%20cells.pdf
http://psasir.upm.edu.my/id/eprint/53037/
http://www.springerplus.com
_version_ 1643835315905888256
score 13.211869

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