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Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Enhancing of the efficient tissue culture protocol for somatic embryos would facilitate the engineered breeding plants program. In this report, we describe the reproducible protocol of Malaysian rice (Oryza sativa L.) cultivar MR219 through somatic embryogenesis. Effect of a wide spectrum of exogene...

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Main Authors: Abiri, Rambod, Mahmood, Maziah, Shaharuddin, Noor Azmi, Balia Yusof, Zetty Norhana, Atabaki, Narges, Musa, Mohamed Hanafi, Sahebi, Mahbod, Azizi, Parisa, Kalhori, Nahid, Valdiani, Alireza
Format: Article
Language:English
Published: Springer 2017
Online Access:http://psasir.upm.edu.my/id/eprint/52454/1/Enhancing%20somatic%20embryogenesis%20of%20Malaysian%20rice%20cultivar%20MR219%20using%20adjuvant%20materials%20in%20a%20high-efficiency%20protocol.pdf
http://psasir.upm.edu.my/id/eprint/52454/
http://rd.springer.com/article/10.1007/s13762-016-1221-y
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spelling my.upm.eprints.524542017-06-06T09:11:25Z http://psasir.upm.edu.my/id/eprint/52454/ Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol Abiri, Rambod Mahmood, Maziah Shaharuddin, Noor Azmi Balia Yusof, Zetty Norhana Atabaki, Narges Musa, Mohamed Hanafi Sahebi, Mahbod Azizi, Parisa Kalhori, Nahid Valdiani, Alireza Enhancing of the efficient tissue culture protocol for somatic embryos would facilitate the engineered breeding plants program. In this report, we describe the reproducible protocol of Malaysian rice (Oryza sativa L.) cultivar MR219 through somatic embryogenesis. Effect of a wide spectrum of exogenesis materials was assessed in three phases, namely callogenesis, proliferation and regeneration. Initially, rice seeds were subjected under various auxin treatments. Secondly, the effect of different concentrations of 2,4-D on callus induction was evaluated. In the next step, the efficiency of different explants was identified. Subsequently, the effects of different auxins, cytokinins, l-proline, casein hydrolysate and potassium metasilicate concentrations on the callus proliferation and regeneration were considered. For the callogenesis phase, 2 mg L−1of 2,4-D and roots were chosen as the best auxin and explant. In the callus proliferation stage, the highest efficiency was observed at week eight in the MS media supplemented with 2 mg L−1 of 2,4-D, 2 mg L−1 of kinetin, 50 mg L−1 of l-proline, 100 mg L−1 of casein hydrolysate and 30 mg L−1 of potassium metasilicate. In the last phase of the research, the MS media added with 3 mg L−1 of kinetin, 30 mg L−1of potassium metasilicate and 2 mg L−1 of NAA were selected. Meanwhile, to promote the roots of regenerated explants, 0.4 mg L−1 of IBA has shown potential as an appropriate activator. Springer 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52454/1/Enhancing%20somatic%20embryogenesis%20of%20Malaysian%20rice%20cultivar%20MR219%20using%20adjuvant%20materials%20in%20a%20high-efficiency%20protocol.pdf Abiri, Rambod and Mahmood, Maziah and Shaharuddin, Noor Azmi and Balia Yusof, Zetty Norhana and Atabaki, Narges and Musa, Mohamed Hanafi and Sahebi, Mahbod and Azizi, Parisa and Kalhori, Nahid and Valdiani, Alireza (2017) Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol. International Journal of Environmental Science and Technology, 14 (5). pp. 1091-1108. ISSN 1735-1472; ESSN: 1735-2630 http://rd.springer.com/article/10.1007/s13762-016-1221-y 10.1007/s13762-016-1221-y
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Enhancing of the efficient tissue culture protocol for somatic embryos would facilitate the engineered breeding plants program. In this report, we describe the reproducible protocol of Malaysian rice (Oryza sativa L.) cultivar MR219 through somatic embryogenesis. Effect of a wide spectrum of exogenesis materials was assessed in three phases, namely callogenesis, proliferation and regeneration. Initially, rice seeds were subjected under various auxin treatments. Secondly, the effect of different concentrations of 2,4-D on callus induction was evaluated. In the next step, the efficiency of different explants was identified. Subsequently, the effects of different auxins, cytokinins, l-proline, casein hydrolysate and potassium metasilicate concentrations on the callus proliferation and regeneration were considered. For the callogenesis phase, 2 mg L−1of 2,4-D and roots were chosen as the best auxin and explant. In the callus proliferation stage, the highest efficiency was observed at week eight in the MS media supplemented with 2 mg L−1 of 2,4-D, 2 mg L−1 of kinetin, 50 mg L−1 of l-proline, 100 mg L−1 of casein hydrolysate and 30 mg L−1 of potassium metasilicate. In the last phase of the research, the MS media added with 3 mg L−1 of kinetin, 30 mg L−1of potassium metasilicate and 2 mg L−1 of NAA were selected. Meanwhile, to promote the roots of regenerated explants, 0.4 mg L−1 of IBA has shown potential as an appropriate activator.
format Article
author Abiri, Rambod
Mahmood, Maziah
Shaharuddin, Noor Azmi
Balia Yusof, Zetty Norhana
Atabaki, Narges
Musa, Mohamed Hanafi
Sahebi, Mahbod
Azizi, Parisa
Kalhori, Nahid
Valdiani, Alireza
spellingShingle Abiri, Rambod
Mahmood, Maziah
Shaharuddin, Noor Azmi
Balia Yusof, Zetty Norhana
Atabaki, Narges
Musa, Mohamed Hanafi
Sahebi, Mahbod
Azizi, Parisa
Kalhori, Nahid
Valdiani, Alireza
Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol
author_facet Abiri, Rambod
Mahmood, Maziah
Shaharuddin, Noor Azmi
Balia Yusof, Zetty Norhana
Atabaki, Narges
Musa, Mohamed Hanafi
Sahebi, Mahbod
Azizi, Parisa
Kalhori, Nahid
Valdiani, Alireza
author_sort Abiri, Rambod
title Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol
title_short Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol
title_full Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol
title_fullStr Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol
title_full_unstemmed Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol
title_sort enhancing somatic embryogenesis of malaysian rice cultivar mr219 using adjuvant materials in a high-efficiency protocol
publisher Springer
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/52454/1/Enhancing%20somatic%20embryogenesis%20of%20Malaysian%20rice%20cultivar%20MR219%20using%20adjuvant%20materials%20in%20a%20high-efficiency%20protocol.pdf
http://psasir.upm.edu.my/id/eprint/52454/
http://rd.springer.com/article/10.1007/s13762-016-1221-y
_version_ 1643835254783344640
score 13.211869

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