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Virulence, host-selective toxin production, and development of three Cochliobolus phytopathogens lacking the Sfp-type 4′-phosphopantetheinyl transferase Ppt1

The Sfp-type 4′-phosphopantetheinyl transferase Ppt1 is required for activation of nonribosomal peptide synthetases, including α-aminoadipate reductase (AAR) for lysine biosynthesis and polyketide synthases, enzymes that biosynthesize peptide and polyketide secondary metabolites, respectively. Delet...

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Main Authors: Mohd Zainudin, Nur Ain Izzati, Condon, Bradford, De Bruyne, Lieselotte, Van Poucke, Christof, Bi, Qing, Li, Wei, Höfte, Monica, Turgeon, B. Gillian
Format: Article
Language:English
Published: APS Press 2015
Online Access:http://psasir.upm.edu.my/id/eprint/52019/1/Virulence%2C%20host-selective%20toxin%20production%2C%20and%20development%20of%20three%20Cochliobolus%20phytopathogens%20lacking%20the%20Sfp-type%204%E2%80%B2-phosphopantetheinyl%20transferase%20Ppt1.pdf
http://psasir.upm.edu.my/id/eprint/52019/
http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-03-15-0068-R
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spelling my.upm.eprints.520192017-05-04T09:00:36Z http://psasir.upm.edu.my/id/eprint/52019/ Virulence, host-selective toxin production, and development of three Cochliobolus phytopathogens lacking the Sfp-type 4′-phosphopantetheinyl transferase Ppt1 Mohd Zainudin, Nur Ain Izzati Condon, Bradford De Bruyne, Lieselotte Van Poucke, Christof Bi, Qing Li, Wei Höfte, Monica Turgeon, B. Gillian The Sfp-type 4′-phosphopantetheinyl transferase Ppt1 is required for activation of nonribosomal peptide synthetases, including α-aminoadipate reductase (AAR) for lysine biosynthesis and polyketide synthases, enzymes that biosynthesize peptide and polyketide secondary metabolites, respectively. Deletion of the PPT1 gene, from the maize pathogen Cochliobolus heterostrophus and the rice pathogen Cochliobolus miyabeanus, yielded strains that were significantly reduced in virulence to their hosts. In addition, ppt1 mutants of C. heterostrophus race T and Cochliobolus victoriae were unable to biosynthesize the host-selective toxins (HST) T-toxin and victorin, respectively, as judged by bioassays. Interestingly, ppt1 mutants of C. miyabeanus were shown to produce tenfold higher levels of the sesterterpene-type non-HST ophiobolin A, as compared with the wild-type strain. The ppt1 strains of all species were also reduced in tolerance to oxidative stress and iron depletion; both phenotypes are associated with inability to produce extracellular siderophores biosynthesized by the nonribosomal peptide synthetase Nps6. Colony surfaces were hydrophilic, a trait previously associated with absence of C. heterostrophus Nps4. Mutants were decreased in asexual sporulation and C. heterostrophus strains were female-sterile in sexual crosses; the latter phenotype was observed previously with mutants lacking Nps2, which produces an intracellular siderophore. As expected, mutants were albino, since they cannot produce the polyketide melanin and were auxotrophic for lysine because they lack an AAR. APS Press 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52019/1/Virulence%2C%20host-selective%20toxin%20production%2C%20and%20development%20of%20three%20Cochliobolus%20phytopathogens%20lacking%20the%20Sfp-type%204%E2%80%B2-phosphopantetheinyl%20transferase%20Ppt1.pdf Mohd Zainudin, Nur Ain Izzati and Condon, Bradford and De Bruyne, Lieselotte and Van Poucke, Christof and Bi, Qing and Li, Wei and Höfte, Monica and Turgeon, B. Gillian (2015) Virulence, host-selective toxin production, and development of three Cochliobolus phytopathogens lacking the Sfp-type 4′-phosphopantetheinyl transferase Ppt1. Molecular Plant-Microbe Interactions, 28 (10). pp. 1130-1141. ISSN 0894-0282 http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-03-15-0068-R 10.1094/MPMI-03-15-0068-R
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The Sfp-type 4′-phosphopantetheinyl transferase Ppt1 is required for activation of nonribosomal peptide synthetases, including α-aminoadipate reductase (AAR) for lysine biosynthesis and polyketide synthases, enzymes that biosynthesize peptide and polyketide secondary metabolites, respectively. Deletion of the PPT1 gene, from the maize pathogen Cochliobolus heterostrophus and the rice pathogen Cochliobolus miyabeanus, yielded strains that were significantly reduced in virulence to their hosts. In addition, ppt1 mutants of C. heterostrophus race T and Cochliobolus victoriae were unable to biosynthesize the host-selective toxins (HST) T-toxin and victorin, respectively, as judged by bioassays. Interestingly, ppt1 mutants of C. miyabeanus were shown to produce tenfold higher levels of the sesterterpene-type non-HST ophiobolin A, as compared with the wild-type strain. The ppt1 strains of all species were also reduced in tolerance to oxidative stress and iron depletion; both phenotypes are associated with inability to produce extracellular siderophores biosynthesized by the nonribosomal peptide synthetase Nps6. Colony surfaces were hydrophilic, a trait previously associated with absence of C. heterostrophus Nps4. Mutants were decreased in asexual sporulation and C. heterostrophus strains were female-sterile in sexual crosses; the latter phenotype was observed previously with mutants lacking Nps2, which produces an intracellular siderophore. As expected, mutants were albino, since they cannot produce the polyketide melanin and were auxotrophic for lysine because they lack an AAR.
format Article
author Mohd Zainudin, Nur Ain Izzati
Condon, Bradford
De Bruyne, Lieselotte
Van Poucke, Christof
Bi, Qing
Li, Wei
Höfte, Monica
Turgeon, B. Gillian
spellingShingle Mohd Zainudin, Nur Ain Izzati
Condon, Bradford
De Bruyne, Lieselotte
Van Poucke, Christof
Bi, Qing
Li, Wei
Höfte, Monica
Turgeon, B. Gillian
Virulence, host-selective toxin production, and development of three Cochliobolus phytopathogens lacking the Sfp-type 4′-phosphopantetheinyl transferase Ppt1
author_facet Mohd Zainudin, Nur Ain Izzati
Condon, Bradford
De Bruyne, Lieselotte
Van Poucke, Christof
Bi, Qing
Li, Wei
Höfte, Monica
Turgeon, B. Gillian
author_sort Mohd Zainudin, Nur Ain Izzati
title Virulence, host-selective toxin production, and development of three Cochliobolus phytopathogens lacking the Sfp-type 4′-phosphopantetheinyl transferase Ppt1
title_short Virulence, host-selective toxin production, and development of three Cochliobolus phytopathogens lacking the Sfp-type 4′-phosphopantetheinyl transferase Ppt1
title_full Virulence, host-selective toxin production, and development of three Cochliobolus phytopathogens lacking the Sfp-type 4′-phosphopantetheinyl transferase Ppt1
title_fullStr Virulence, host-selective toxin production, and development of three Cochliobolus phytopathogens lacking the Sfp-type 4′-phosphopantetheinyl transferase Ppt1
title_full_unstemmed Virulence, host-selective toxin production, and development of three Cochliobolus phytopathogens lacking the Sfp-type 4′-phosphopantetheinyl transferase Ppt1
title_sort virulence, host-selective toxin production, and development of three cochliobolus phytopathogens lacking the sfp-type 4′-phosphopantetheinyl transferase ppt1
publisher APS Press
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/52019/1/Virulence%2C%20host-selective%20toxin%20production%2C%20and%20development%20of%20three%20Cochliobolus%20phytopathogens%20lacking%20the%20Sfp-type%204%E2%80%B2-phosphopantetheinyl%20transferase%20Ppt1.pdf
http://psasir.upm.edu.my/id/eprint/52019/
http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-03-15-0068-R
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score 13.211869

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