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Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa)

A total of 35 Kanagawa-negative strains of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) were investigated by randomly amplified polymorphic DNA fingerprinting with three primers and their plasmid profiles. Eighteen strains carried small plasmid(s) of 2.4 to 7.3 kb that enabled the...

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Main Authors: R., Son, Nasreldin, E. H., H., Zaiton, L., Samuel, Rusul, G., Nimita, F.
Format: Article
Language:English
English
Published: Rapid Science Publishers 1998
Online Access:http://psasir.upm.edu.my/id/eprint/51780/1/51780.pdf
http://psasir.upm.edu.my/id/eprint/51780/7/A_1008841514368.pdf
http://psasir.upm.edu.my/id/eprint/51780/
https://rd.springer.com/article/10.1023/A:1008841514368
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spelling my.upm.eprints.517802024-08-08T00:08:02Z http://psasir.upm.edu.my/id/eprint/51780/ Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) R., Son Nasreldin, E. H. H., Zaiton L., Samuel Rusul, G. Nimita, F. A total of 35 Kanagawa-negative strains of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) were investigated by randomly amplified polymorphic DNA fingerprinting with three primers and their plasmid profiles. Eighteen strains carried small plasmid(s) of 2.4 to 7.3 kb that enabled the V. parahaemolyticus to be grouped into eight plasmid patterns. The three primers generated polymorphisms in all 35 strains of V. parahaemolyticus tested, producing bands ranging from 0.25 to 3.9 kb. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested, and that cockles in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. Rapid Science Publishers 1998 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/51780/1/51780.pdf text en http://psasir.upm.edu.my/id/eprint/51780/7/A_1008841514368.pdf R., Son and Nasreldin, E. H. and H., Zaiton and L., Samuel and Rusul, G. and Nimita, F. (1998) Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa). World Journal of Microbiology and Biotechnology, 14 (6). pp. 895-901. ISSN 0959-3993; ESSN: 1573-0972 https://rd.springer.com/article/10.1023/A:1008841514368 10.1023/A:1008841514368
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description A total of 35 Kanagawa-negative strains of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) were investigated by randomly amplified polymorphic DNA fingerprinting with three primers and their plasmid profiles. Eighteen strains carried small plasmid(s) of 2.4 to 7.3 kb that enabled the V. parahaemolyticus to be grouped into eight plasmid patterns. The three primers generated polymorphisms in all 35 strains of V. parahaemolyticus tested, producing bands ranging from 0.25 to 3.9 kb. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested, and that cockles in the study area are populated by genetically polymorphic strains of V. parahaemolyticus.
format Article
author R., Son
Nasreldin, E. H.
H., Zaiton
L., Samuel
Rusul, G.
Nimita, F.
spellingShingle R., Son
Nasreldin, E. H.
H., Zaiton
L., Samuel
Rusul, G.
Nimita, F.
Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa)
author_facet R., Son
Nasreldin, E. H.
H., Zaiton
L., Samuel
Rusul, G.
Nimita, F.
author_sort R., Son
title Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa)
title_short Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa)
title_full Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa)
title_fullStr Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa)
title_full_unstemmed Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa)
title_sort use of randomly amplified polymorphic dna analysis to differentiate isolates of vibrio parahaemolyticus from cockles (anadara granosa)
publisher Rapid Science Publishers
publishDate 1998
url http://psasir.upm.edu.my/id/eprint/51780/1/51780.pdf
http://psasir.upm.edu.my/id/eprint/51780/7/A_1008841514368.pdf
http://psasir.upm.edu.my/id/eprint/51780/
https://rd.springer.com/article/10.1023/A:1008841514368
_version_ 1807051115991138304
score 13.211869

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