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Saccharification of pretreated oil palm empty fruit bunch fiber using cellulase of Chaetomium globosum

The effectiveness of different chemical and physical pretreatments to alter cellulose structure and to reduce hemicellullose and lignin contents in oil palm empty fruit bunch (OPEFB) fibers for subsequent use as substrate for enzymatic saccharification was studied. The saccharification was carried o...

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Main Authors: Md. Shah, Umi Kalsom, Ariff, Arbakariya, Abdul Karim, Mohamed Ismail
Format: Article
Language:English
Published: American Chemical Society 1998
Online Access:http://psasir.upm.edu.my/id/eprint/51670/1/51670.pdf
http://psasir.upm.edu.my/id/eprint/51670/
http://pubs.acs.org/doi/abs/10.1021/jf971098c
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spelling my.upm.eprints.516702017-09-05T08:38:40Z http://psasir.upm.edu.my/id/eprint/51670/ Saccharification of pretreated oil palm empty fruit bunch fiber using cellulase of Chaetomium globosum Md. Shah, Umi Kalsom Ariff, Arbakariya Abdul Karim, Mohamed Ismail The effectiveness of different chemical and physical pretreatments to alter cellulose structure and to reduce hemicellullose and lignin contents in oil palm empty fruit bunch (OPEFB) fibers for subsequent use as substrate for enzymatic saccharification was studied. The saccharification was carried out using concentrated cellulase preparation from culture broth of Chaetomium globosum strain 414 containing 10 U/mL FPase, 285 U/mL CMCase, and 60 U/mL β-glucosidase. The use of 0.5% NaOH to treat OPEFB by soaking at 30 °C for 4 h gave the highest rate and degree of hydrolysis followed by 0.5% HNO3, HCl, EDA, and EDTA. Autoclaving the chemically treated OPEFB fiber at 121 °C for 5 min improved the hydrolysis by 2-fold. The improvement in hydrolysis was related to a decrease in the hemicellulose and lignin contents and an increase in the cellulose content. The qualitative hydrolysis yield for autoclaved OPEFB fiber treated with 2% NaOH was 85.9%. During saccharification of OPEFB using cellulase of C. globosum, the amount of glucose produced was higher while the amount of cellobiose produced was lower than those obtained with commercial cellulase of Trichoderma viride. American Chemical Society 1998 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51670/1/51670.pdf Md. Shah, Umi Kalsom and Ariff, Arbakariya and Abdul Karim, Mohamed Ismail (1998) Saccharification of pretreated oil palm empty fruit bunch fiber using cellulase of Chaetomium globosum. Journal of Agricultural and Food Chemistry, 46 (8). pp. 3359-3364. ISSN 0021-8561; ESSN: 1520-5118 http://pubs.acs.org/doi/abs/10.1021/jf971098c 10.1021/jf971098c
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The effectiveness of different chemical and physical pretreatments to alter cellulose structure and to reduce hemicellullose and lignin contents in oil palm empty fruit bunch (OPEFB) fibers for subsequent use as substrate for enzymatic saccharification was studied. The saccharification was carried out using concentrated cellulase preparation from culture broth of Chaetomium globosum strain 414 containing 10 U/mL FPase, 285 U/mL CMCase, and 60 U/mL β-glucosidase. The use of 0.5% NaOH to treat OPEFB by soaking at 30 °C for 4 h gave the highest rate and degree of hydrolysis followed by 0.5% HNO3, HCl, EDA, and EDTA. Autoclaving the chemically treated OPEFB fiber at 121 °C for 5 min improved the hydrolysis by 2-fold. The improvement in hydrolysis was related to a decrease in the hemicellulose and lignin contents and an increase in the cellulose content. The qualitative hydrolysis yield for autoclaved OPEFB fiber treated with 2% NaOH was 85.9%. During saccharification of OPEFB using cellulase of C. globosum, the amount of glucose produced was higher while the amount of cellobiose produced was lower than those obtained with commercial cellulase of Trichoderma viride.
format Article
author Md. Shah, Umi Kalsom
Ariff, Arbakariya
Abdul Karim, Mohamed Ismail
spellingShingle Md. Shah, Umi Kalsom
Ariff, Arbakariya
Abdul Karim, Mohamed Ismail
Saccharification of pretreated oil palm empty fruit bunch fiber using cellulase of Chaetomium globosum
author_facet Md. Shah, Umi Kalsom
Ariff, Arbakariya
Abdul Karim, Mohamed Ismail
author_sort Md. Shah, Umi Kalsom
title Saccharification of pretreated oil palm empty fruit bunch fiber using cellulase of Chaetomium globosum
title_short Saccharification of pretreated oil palm empty fruit bunch fiber using cellulase of Chaetomium globosum
title_full Saccharification of pretreated oil palm empty fruit bunch fiber using cellulase of Chaetomium globosum
title_fullStr Saccharification of pretreated oil palm empty fruit bunch fiber using cellulase of Chaetomium globosum
title_full_unstemmed Saccharification of pretreated oil palm empty fruit bunch fiber using cellulase of Chaetomium globosum
title_sort saccharification of pretreated oil palm empty fruit bunch fiber using cellulase of chaetomium globosum
publisher American Chemical Society
publishDate 1998
url http://psasir.upm.edu.my/id/eprint/51670/1/51670.pdf
http://psasir.upm.edu.my/id/eprint/51670/
http://pubs.acs.org/doi/abs/10.1021/jf971098c
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score 13.211869

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