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Molecular characterization of a Toxocara variant from cats in Kuala Lumpur, Malaysia

The ascaridoid nematode of cats from Kuala Lumpur, Malaysia, previously identified morphologically as Toxocara canis, was characterized using a molecular approach. The nuclear ribosomal DNA (rDNA) region spanning the first internal transcribed spacer (ITS-1), the 5.8S gene and the second internal tr...

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Main Authors: Zhu, Xing Quan, Jacobs, Dennis E., Chilton, Neil B., Abdullah Sani, Rehana, Cheng, Nadzariah A. B. Y., Gasser, Robin B.
Format: Article
Language:English
Published: Cambridge University Press 1998
Online Access:http://psasir.upm.edu.my/id/eprint/51666/1/51666.pdf
http://psasir.upm.edu.my/id/eprint/51666/
https://www.cambridge.org/core/journals/parasitology/article/molecular-characterization-of-a-toxocara-variant-from-cats-in-kuala-lumpur-malaysia/2C4F740FD63CD73EAF4EFA10DEDA0A3A
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spelling my.upm.eprints.516662017-07-04T04:14:35Z http://psasir.upm.edu.my/id/eprint/51666/ Molecular characterization of a Toxocara variant from cats in Kuala Lumpur, Malaysia Zhu, Xing Quan Jacobs, Dennis E. Chilton, Neil B. Abdullah Sani, Rehana Cheng, Nadzariah A. B. Y. Gasser, Robin B. The ascaridoid nematode of cats from Kuala Lumpur, Malaysia, previously identified morphologically as Toxocara canis, was characterized using a molecular approach. The nuclear ribosomal DNA (rDNA) region spanning the first internal transcribed spacer (ITS-1), the 5.8S gene and the second internal transcribed spacer (ITS-2) was amplified and sequenced. The sequences for the parasite from Malaysian cats were compared with those for T. canis and T. cati. The sequence data showed that this taxon was genetically more similar to T. cati than to T. canis in the ITS-1, 5.8S and ITS-2. Differences in the ITS-1 and ITS-2 sequences between the taxa (9.4-26.1%) were markedly higher than variation between samples within T. canis and T. cati (0-2.9%). The sequence data demonstrate that the parasite from Malaysian cats is neither T. canis nor T. cati and indicate that it is a distinct species. Based on these data, PCR-linked restriction fragment length polymorphism (RFLP) and single-strand conformation polymorphism (SSCP) methods were employed for the unequivocal differentiation of the Toxocara variant from T. canis and T. cati. These methods should provide valuable tools for studying the life-cycle, transmission pattern(s) and zoonotic potential of this parasite. Cambridge University Press 1998 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51666/1/51666.pdf Zhu, Xing Quan and Jacobs, Dennis E. and Chilton, Neil B. and Abdullah Sani, Rehana and Cheng, Nadzariah A. B. Y. and Gasser, Robin B. (1998) Molecular characterization of a Toxocara variant from cats in Kuala Lumpur, Malaysia. Parasitology, 117 (2). pp. 155-164. ISSN 0031-1820; ESSN: 1469-8161 https://www.cambridge.org/core/journals/parasitology/article/molecular-characterization-of-a-toxocara-variant-from-cats-in-kuala-lumpur-malaysia/2C4F740FD63CD73EAF4EFA10DEDA0A3A 10.1017/S0031182098002856
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The ascaridoid nematode of cats from Kuala Lumpur, Malaysia, previously identified morphologically as Toxocara canis, was characterized using a molecular approach. The nuclear ribosomal DNA (rDNA) region spanning the first internal transcribed spacer (ITS-1), the 5.8S gene and the second internal transcribed spacer (ITS-2) was amplified and sequenced. The sequences for the parasite from Malaysian cats were compared with those for T. canis and T. cati. The sequence data showed that this taxon was genetically more similar to T. cati than to T. canis in the ITS-1, 5.8S and ITS-2. Differences in the ITS-1 and ITS-2 sequences between the taxa (9.4-26.1%) were markedly higher than variation between samples within T. canis and T. cati (0-2.9%). The sequence data demonstrate that the parasite from Malaysian cats is neither T. canis nor T. cati and indicate that it is a distinct species. Based on these data, PCR-linked restriction fragment length polymorphism (RFLP) and single-strand conformation polymorphism (SSCP) methods were employed for the unequivocal differentiation of the Toxocara variant from T. canis and T. cati. These methods should provide valuable tools for studying the life-cycle, transmission pattern(s) and zoonotic potential of this parasite.
format Article
author Zhu, Xing Quan
Jacobs, Dennis E.
Chilton, Neil B.
Abdullah Sani, Rehana
Cheng, Nadzariah A. B. Y.
Gasser, Robin B.
spellingShingle Zhu, Xing Quan
Jacobs, Dennis E.
Chilton, Neil B.
Abdullah Sani, Rehana
Cheng, Nadzariah A. B. Y.
Gasser, Robin B.
Molecular characterization of a Toxocara variant from cats in Kuala Lumpur, Malaysia
author_facet Zhu, Xing Quan
Jacobs, Dennis E.
Chilton, Neil B.
Abdullah Sani, Rehana
Cheng, Nadzariah A. B. Y.
Gasser, Robin B.
author_sort Zhu, Xing Quan
title Molecular characterization of a Toxocara variant from cats in Kuala Lumpur, Malaysia
title_short Molecular characterization of a Toxocara variant from cats in Kuala Lumpur, Malaysia
title_full Molecular characterization of a Toxocara variant from cats in Kuala Lumpur, Malaysia
title_fullStr Molecular characterization of a Toxocara variant from cats in Kuala Lumpur, Malaysia
title_full_unstemmed Molecular characterization of a Toxocara variant from cats in Kuala Lumpur, Malaysia
title_sort molecular characterization of a toxocara variant from cats in kuala lumpur, malaysia
publisher Cambridge University Press
publishDate 1998
url http://psasir.upm.edu.my/id/eprint/51666/1/51666.pdf
http://psasir.upm.edu.my/id/eprint/51666/
https://www.cambridge.org/core/journals/parasitology/article/molecular-characterization-of-a-toxocara-variant-from-cats-in-kuala-lumpur-malaysia/2C4F740FD63CD73EAF4EFA10DEDA0A3A
_version_ 1643835020164464640
score 13.211869

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