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Evaluation of primer sequence targeting inv A gene of Salmonella sp. by in silico PCR

In silico PCR via algorithm and computer simulation aim to provide an easy way to analyse and obtain the theoretical PCR results we may expect from DNA, by using up to date bacterial genomes sequences. In this study, primer set invA-F and invA-R targeting Salmonella invasion gene, invA, was evaluate...

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Main Authors: Abdul Talib, Mohd Afendy, M., Noor Azlina, Radu, Son
Format: Article
Language:English
Published: American-Eurasian Network for Scientific Information 2015
Online Access:http://psasir.upm.edu.my/id/eprint/51640/1/Evaluation%20of%20primer%20sequence%20targeting%20inv%20A%20gene%20of%20Salmonella%20sp.%20by%20in%20silico%20PCR.pdf
http://psasir.upm.edu.my/id/eprint/51640/
http://ajbasweb.com/old/ajbas_March_2015.html
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spelling my.upm.eprints.516402017-04-05T05:31:58Z http://psasir.upm.edu.my/id/eprint/51640/ Evaluation of primer sequence targeting inv A gene of Salmonella sp. by in silico PCR Abdul Talib, Mohd Afendy M., Noor Azlina Radu, Son In silico PCR via algorithm and computer simulation aim to provide an easy way to analyse and obtain the theoretical PCR results we may expect from DNA, by using up to date bacterial genomes sequences. In this study, primer set invA-F and invA-R targeting Salmonella invasion gene, invA, was evaluated by in-silico PCR amplification against prokaryotic genome of major foodborne pathogens. A total of 127 strains of bacterial genome sequences from Salmonella sp.(27), Escherichia sp.(59), Listeria sp.(26) and Campylobacter sp.(15) were used as DNA templates in the PCR simulation analysis. The primer set simulatively amplified a single band of 285 bp PCR product with all 25 strains of Salmonella enterica subsp. enterica, whereas no amplification is produced with Salmonella bongori and Salmonella enterica subsp. arizonae. There was no cross-reaction obtained with other bacterial genomes indicated that the primer set is specific to Salmonella enterica subsp. enterica only. PCR experiments using invA-F and invA-R that was carried out in the laboratory had successfully amplified the 285 bp amplicons using DNA from S. Typhimurium, S. Enteritidis, S. Polarum and S. Gallinarum. American-Eurasian Network for Scientific Information 2015-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51640/1/Evaluation%20of%20primer%20sequence%20targeting%20inv%20A%20gene%20of%20Salmonella%20sp.%20by%20in%20silico%20PCR.pdf Abdul Talib, Mohd Afendy and M., Noor Azlina and Radu, Son (2015) Evaluation of primer sequence targeting inv A gene of Salmonella sp. by in silico PCR. Australian Journal of Basic and Applied Sciences, 9 (5). pp. 520-523. ISSN 1991-8178 http://ajbasweb.com/old/ajbas_March_2015.html
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description In silico PCR via algorithm and computer simulation aim to provide an easy way to analyse and obtain the theoretical PCR results we may expect from DNA, by using up to date bacterial genomes sequences. In this study, primer set invA-F and invA-R targeting Salmonella invasion gene, invA, was evaluated by in-silico PCR amplification against prokaryotic genome of major foodborne pathogens. A total of 127 strains of bacterial genome sequences from Salmonella sp.(27), Escherichia sp.(59), Listeria sp.(26) and Campylobacter sp.(15) were used as DNA templates in the PCR simulation analysis. The primer set simulatively amplified a single band of 285 bp PCR product with all 25 strains of Salmonella enterica subsp. enterica, whereas no amplification is produced with Salmonella bongori and Salmonella enterica subsp. arizonae. There was no cross-reaction obtained with other bacterial genomes indicated that the primer set is specific to Salmonella enterica subsp. enterica only. PCR experiments using invA-F and invA-R that was carried out in the laboratory had successfully amplified the 285 bp amplicons using DNA from S. Typhimurium, S. Enteritidis, S. Polarum and S. Gallinarum.
format Article
author Abdul Talib, Mohd Afendy
M., Noor Azlina
Radu, Son
spellingShingle Abdul Talib, Mohd Afendy
M., Noor Azlina
Radu, Son
Evaluation of primer sequence targeting inv A gene of Salmonella sp. by in silico PCR
author_facet Abdul Talib, Mohd Afendy
M., Noor Azlina
Radu, Son
author_sort Abdul Talib, Mohd Afendy
title Evaluation of primer sequence targeting inv A gene of Salmonella sp. by in silico PCR
title_short Evaluation of primer sequence targeting inv A gene of Salmonella sp. by in silico PCR
title_full Evaluation of primer sequence targeting inv A gene of Salmonella sp. by in silico PCR
title_fullStr Evaluation of primer sequence targeting inv A gene of Salmonella sp. by in silico PCR
title_full_unstemmed Evaluation of primer sequence targeting inv A gene of Salmonella sp. by in silico PCR
title_sort evaluation of primer sequence targeting inv a gene of salmonella sp. by in silico pcr
publisher American-Eurasian Network for Scientific Information
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/51640/1/Evaluation%20of%20primer%20sequence%20targeting%20inv%20A%20gene%20of%20Salmonella%20sp.%20by%20in%20silico%20PCR.pdf
http://psasir.upm.edu.my/id/eprint/51640/
http://ajbasweb.com/old/ajbas_March_2015.html
_version_ 1643835013011079168
score 13.211869

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