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Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis

The ethanol extract from the leaves of Juniperus chinensis was found to be cytotoxic towards HeLa cells. Bioassay-guided fractionation of the EtOAc soluble faction directed by the microtitration cytotoxic assay revealed that the cytotoxic compound was deoxypodophyllotoxin. All the tumour cell lines...

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Main Authors: Ali, Abdul Manaf, Ismail, Intan Safinar, Mohamed Mackeen, Muhammad Mukram, El-Sharkawy, Saleh Hassan, Takahata, Kyoya, Kanzaki, Hiroshi, Kawazu, Kazuyoshi
Format: Article
Language:English
Published: Korean Society of Pharmacognosy 1998
Online Access:http://psasir.upm.edu.my/id/eprint/51340/1/51340.pdf
http://psasir.upm.edu.my/id/eprint/51340/
http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=E1HSBY_1998_v4n3_180
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spelling my.upm.eprints.513402017-07-04T03:56:37Z http://psasir.upm.edu.my/id/eprint/51340/ Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis Ali, Abdul Manaf Ismail, Intan Safinar Mohamed Mackeen, Muhammad Mukram El-Sharkawy, Saleh Hassan Takahata, Kyoya Kanzaki, Hiroshi Kawazu, Kazuyoshi The ethanol extract from the leaves of Juniperus chinensis was found to be cytotoxic towards HeLa cells. Bioassay-guided fractionation of the EtOAc soluble faction directed by the microtitration cytotoxic assay revealed that the cytotoxic compound was deoxypodophyllotoxin. All the tumour cell lines tested (KU8112F-chronic mylogeneous leukemia, TK 10-renal carcinoma, UACC 62-melanoma and CEM-SS - T-lymphoblastic leukemia) were found to be susceptible to deoxypodophyllotoxin, however, the minimum effective concentration (MEC) required to reduce the cell population by 100 percent was different between cell lines. Korean Society of Pharmacognosy 1998 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51340/1/51340.pdf Ali, Abdul Manaf and Ismail, Intan Safinar and Mohamed Mackeen, Muhammad Mukram and El-Sharkawy, Saleh Hassan and Takahata, Kyoya and Kanzaki, Hiroshi and Kawazu, Kazuyoshi (1998) Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis. Natural Product Sciences, 4 (3). pp. 180-183. ISSN 1226-3907 http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=E1HSBY_1998_v4n3_180
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The ethanol extract from the leaves of Juniperus chinensis was found to be cytotoxic towards HeLa cells. Bioassay-guided fractionation of the EtOAc soluble faction directed by the microtitration cytotoxic assay revealed that the cytotoxic compound was deoxypodophyllotoxin. All the tumour cell lines tested (KU8112F-chronic mylogeneous leukemia, TK 10-renal carcinoma, UACC 62-melanoma and CEM-SS - T-lymphoblastic leukemia) were found to be susceptible to deoxypodophyllotoxin, however, the minimum effective concentration (MEC) required to reduce the cell population by 100 percent was different between cell lines.
format Article
author Ali, Abdul Manaf
Ismail, Intan Safinar
Mohamed Mackeen, Muhammad Mukram
El-Sharkawy, Saleh Hassan
Takahata, Kyoya
Kanzaki, Hiroshi
Kawazu, Kazuyoshi
spellingShingle Ali, Abdul Manaf
Ismail, Intan Safinar
Mohamed Mackeen, Muhammad Mukram
El-Sharkawy, Saleh Hassan
Takahata, Kyoya
Kanzaki, Hiroshi
Kawazu, Kazuyoshi
Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis
author_facet Ali, Abdul Manaf
Ismail, Intan Safinar
Mohamed Mackeen, Muhammad Mukram
El-Sharkawy, Saleh Hassan
Takahata, Kyoya
Kanzaki, Hiroshi
Kawazu, Kazuyoshi
author_sort Ali, Abdul Manaf
title Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis
title_short Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis
title_full Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis
title_fullStr Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis
title_full_unstemmed Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis
title_sort bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of juniperus chinensis
publisher Korean Society of Pharmacognosy
publishDate 1998
url http://psasir.upm.edu.my/id/eprint/51340/1/51340.pdf
http://psasir.upm.edu.my/id/eprint/51340/
http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=E1HSBY_1998_v4n3_180
_version_ 1643834926332641280
score 13.211869

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