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Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)

Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into th...

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Main Authors: Wan Omar, Wan Siti Atikah, Sulaiman, Ahmad Ziad, Ajit @ Abd Aziz, Azilah, Chisti, Yusuf, Ab Rahim, Mohd Hairul, Leow, Adam Thean Chor
Format: Conference or Workshop Item
Language:English
Published: 2016
Online Access:http://psasir.upm.edu.my/id/eprint/51310/1/P011%20pg71-76.pdf
http://psasir.upm.edu.my/id/eprint/51310/
http://ee.ump.edu.my/ncon/download/
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spelling my.upm.eprints.513102017-04-03T07:48:05Z http://psasir.upm.edu.my/id/eprint/51310/ Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) Wan Omar, Wan Siti Atikah Sulaiman, Ahmad Ziad Ajit @ Abd Aziz, Azilah Chisti, Yusuf Ab Rahim, Mohd Hairul Leow, Adam Thean Chor Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into the same strain of competent cells would assist in research assessment. This study focused on the transformation of recombinant lipase from two different sources into Escherichia coli competent cells, BL21 (DE3). The recombinant cells, pUC8.2-14 (American Type Culture Collection (ATCC)®68046™) was harbouring Rhizopus (delemar) oryzae lipase and E. coli Top 10 pTrcHis was harbouring Staphylococcus hyicus lipase, respectively. The transformation is involved preparation of competent cells, polymerase chain reaction (PCR) and DNA sequencing. The DNA sequences obtained was inserted into Basic Local Alignment Search Tool (BLAST), under the databases of National Centre for Biotechnology Information (NCBI), USA. The findings show the respective plasmids were successfully carried by the E. coli BL21 (DE3) for further investigation. 2016 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51310/1/P011%20pg71-76.pdf Wan Omar, Wan Siti Atikah and Sulaiman, Ahmad Ziad and Ajit @ Abd Aziz, Azilah and Chisti, Yusuf and Ab Rahim, Mohd Hairul and Leow, Adam Thean Chor (2016) Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3). In: 3rd National Conference for Postgraduate Research (NCON-PGR2016), 24-25 Sept. 2016, Universiti Malaysia Pahang, Pekan, Pahang. (pp. 71-76). http://ee.ump.edu.my/ncon/download/
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into the same strain of competent cells would assist in research assessment. This study focused on the transformation of recombinant lipase from two different sources into Escherichia coli competent cells, BL21 (DE3). The recombinant cells, pUC8.2-14 (American Type Culture Collection (ATCC)®68046™) was harbouring Rhizopus (delemar) oryzae lipase and E. coli Top 10 pTrcHis was harbouring Staphylococcus hyicus lipase, respectively. The transformation is involved preparation of competent cells, polymerase chain reaction (PCR) and DNA sequencing. The DNA sequences obtained was inserted into Basic Local Alignment Search Tool (BLAST), under the databases of National Centre for Biotechnology Information (NCBI), USA. The findings show the respective plasmids were successfully carried by the E. coli BL21 (DE3) for further investigation.
format Conference or Workshop Item
author Wan Omar, Wan Siti Atikah
Sulaiman, Ahmad Ziad
Ajit @ Abd Aziz, Azilah
Chisti, Yusuf
Ab Rahim, Mohd Hairul
Leow, Adam Thean Chor
spellingShingle Wan Omar, Wan Siti Atikah
Sulaiman, Ahmad Ziad
Ajit @ Abd Aziz, Azilah
Chisti, Yusuf
Ab Rahim, Mohd Hairul
Leow, Adam Thean Chor
Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
author_facet Wan Omar, Wan Siti Atikah
Sulaiman, Ahmad Ziad
Ajit @ Abd Aziz, Azilah
Chisti, Yusuf
Ab Rahim, Mohd Hairul
Leow, Adam Thean Chor
author_sort Wan Omar, Wan Siti Atikah
title Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
title_short Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
title_full Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
title_fullStr Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
title_full_unstemmed Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
title_sort transformation of ptrchis and puc8.2-14 into escherichia coli bl21 (de3)
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/51310/1/P011%20pg71-76.pdf
http://psasir.upm.edu.my/id/eprint/51310/
http://ee.ump.edu.my/ncon/download/
_version_ 1643834917460639744
score 13.211869

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