Cover Image

Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro

BACKGROUND: Condensed tannin (CT) fractions of different molecular weights (MWs) may affect rumen microbial metabolism by altering bacterial diversity. In this study the effects of unfractionated CTs (F0) and five CT fractions (F1–F5) of different MWs (F1, 1265.8 Da; F2, 1028.6 Da; F3, 652.2 Da; F4,...

Full description

Saved in:
Bibliographic Details
Main Authors: Poothan Mookiah, Saminathan, Sieo, Chin Chin, Gan, Han Ming, Ravi, Sharanya, Venkatachalam, Karthikkumar, Abdullah, Norhani, Wong, Clemente Michael Vui Ling, Ho, Yin Wan
Format: Article
Language:English
Published: Society of Chemical Industry 2016
Online Access:http://psasir.upm.edu.my/id/eprint/51174/1/Modulatory%20effects%20of%20condensed%20tannin%20fractions%20of%20different%20molecular%20weights%20from%20a%20Leucaena%20leucocephala%20hybrid%20on%20the%20bovine%20rumen%20bacterial%20community%20in%20vitro.pdf
http://psasir.upm.edu.my/id/eprint/51174/
http://onlinelibrary.wiley.com/wol1/doi/10.1002/jsfa.7674/abstract
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.51174
record_format eprints
spelling my.upm.eprints.511742017-05-02T04:40:22Z http://psasir.upm.edu.my/id/eprint/51174/ Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro Poothan Mookiah, Saminathan Sieo, Chin Chin Gan, Han Ming Ravi, Sharanya Venkatachalam, Karthikkumar Abdullah, Norhani Wong, Clemente Michael Vui Ling Ho, Yin Wan BACKGROUND: Condensed tannin (CT) fractions of different molecular weights (MWs) may affect rumen microbial metabolism by altering bacterial diversity. In this study the effects of unfractionated CTs (F0) and five CT fractions (F1–F5) of different MWs (F1, 1265.8 Da; F2, 1028.6 Da; F3, 652.2 Da; F4, 562.2 Da; F5, 469.6 Da) from Leucaena leucocephala hybrid-Rendang (LLR) on the structure and diversity of the rumen bacterial community were investigated in vitro. RESULTS: Real-time polymerase chain reaction assay showed that the total bacterial population was not significantly (P > 0.05) different among the dietary treatments. Inclusion of higher-MW CT fractions F1 and F2 significantly (P < 0.05) increased the Fibrobacter succinogenes population compared with F0 and CT fractions F3–F5. Although inclusion of F0 and CT fractions (F1–F5) significantly (P < 0.05) decreased the Ruminococcus flavefaciens population, there was no effect on the Ruminococcus albus population when compared with the control (without CTs). High-throughput sequencing of the V3 region of 16S rRNA showed that the relative abundance of genera Prevotella and unclassified Clostridiales was significantly (P < 0.05) decreased, corresponding with increasing MW of CT fractions, whereas cellulolytic bacteria of the genus Fibrobacter were significantly (P < 0.05) increased. Inclusion of higher-MW CT fractions F1 and/or F2 decreased the relative abundance of minor genera such as Ruminococcus, Streptococcus, Clostridium XIVa and Anaeroplasma but increased the relative abundance of Acinetobacter, Treponema, Selenomonas, Succiniclasticum and unclassified Spirochaetales compared with the control and lower-MW CT fractions. CONCLUSION: This study indicates that CT fractions of different MWs may play an important role in altering the structure and diversity of the rumen bacterial community in vitro, and the impact was more pronounced for CT fractions with higher MW. Society of Chemical Industry 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51174/1/Modulatory%20effects%20of%20condensed%20tannin%20fractions%20of%20different%20molecular%20weights%20from%20a%20Leucaena%20leucocephala%20hybrid%20on%20the%20bovine%20rumen%20bacterial%20community%20in%20vitro.pdf Poothan Mookiah, Saminathan and Sieo, Chin Chin and Gan, Han Ming and Ravi, Sharanya and Venkatachalam, Karthikkumar and Abdullah, Norhani and Wong, Clemente Michael Vui Ling and Ho, Yin Wan (2016) Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro. Journal of the Science of Food and Agriculture, 96 (13). pp. 4565-4574. ISSN 0022-5142; ESSN: 1097-0010 http://onlinelibrary.wiley.com/wol1/doi/10.1002/jsfa.7674/abstract 10.1002/jsfa.7674
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description BACKGROUND: Condensed tannin (CT) fractions of different molecular weights (MWs) may affect rumen microbial metabolism by altering bacterial diversity. In this study the effects of unfractionated CTs (F0) and five CT fractions (F1–F5) of different MWs (F1, 1265.8 Da; F2, 1028.6 Da; F3, 652.2 Da; F4, 562.2 Da; F5, 469.6 Da) from Leucaena leucocephala hybrid-Rendang (LLR) on the structure and diversity of the rumen bacterial community were investigated in vitro. RESULTS: Real-time polymerase chain reaction assay showed that the total bacterial population was not significantly (P > 0.05) different among the dietary treatments. Inclusion of higher-MW CT fractions F1 and F2 significantly (P < 0.05) increased the Fibrobacter succinogenes population compared with F0 and CT fractions F3–F5. Although inclusion of F0 and CT fractions (F1–F5) significantly (P < 0.05) decreased the Ruminococcus flavefaciens population, there was no effect on the Ruminococcus albus population when compared with the control (without CTs). High-throughput sequencing of the V3 region of 16S rRNA showed that the relative abundance of genera Prevotella and unclassified Clostridiales was significantly (P < 0.05) decreased, corresponding with increasing MW of CT fractions, whereas cellulolytic bacteria of the genus Fibrobacter were significantly (P < 0.05) increased. Inclusion of higher-MW CT fractions F1 and/or F2 decreased the relative abundance of minor genera such as Ruminococcus, Streptococcus, Clostridium XIVa and Anaeroplasma but increased the relative abundance of Acinetobacter, Treponema, Selenomonas, Succiniclasticum and unclassified Spirochaetales compared with the control and lower-MW CT fractions. CONCLUSION: This study indicates that CT fractions of different MWs may play an important role in altering the structure and diversity of the rumen bacterial community in vitro, and the impact was more pronounced for CT fractions with higher MW.
format Article
author Poothan Mookiah, Saminathan
Sieo, Chin Chin
Gan, Han Ming
Ravi, Sharanya
Venkatachalam, Karthikkumar
Abdullah, Norhani
Wong, Clemente Michael Vui Ling
Ho, Yin Wan
spellingShingle Poothan Mookiah, Saminathan
Sieo, Chin Chin
Gan, Han Ming
Ravi, Sharanya
Venkatachalam, Karthikkumar
Abdullah, Norhani
Wong, Clemente Michael Vui Ling
Ho, Yin Wan
Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro
author_facet Poothan Mookiah, Saminathan
Sieo, Chin Chin
Gan, Han Ming
Ravi, Sharanya
Venkatachalam, Karthikkumar
Abdullah, Norhani
Wong, Clemente Michael Vui Ling
Ho, Yin Wan
author_sort Poothan Mookiah, Saminathan
title Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro
title_short Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro
title_full Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro
title_fullStr Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro
title_full_unstemmed Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro
title_sort modulatory effects of condensed tannin fractions of different molecular weights from a leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro
publisher Society of Chemical Industry
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/51174/1/Modulatory%20effects%20of%20condensed%20tannin%20fractions%20of%20different%20molecular%20weights%20from%20a%20Leucaena%20leucocephala%20hybrid%20on%20the%20bovine%20rumen%20bacterial%20community%20in%20vitro.pdf
http://psasir.upm.edu.my/id/eprint/51174/
http://onlinelibrary.wiley.com/wol1/doi/10.1002/jsfa.7674/abstract
_version_ 1643834878263820288
score 13.211869

Similar Items