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Production of urokinase by HT 1080 human kidney cell line

Studies were carried out in T-flasks and bioreactor to produce urokinase enzyme using HT 1080 human kidney cell line. While growing the cell line it has been observed that the lag phase is reduced considerably in the bioreactor as compared to T-flask culture. The HT 1080 cell adhesion rate and uroki...

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Main Authors: Roychoudhury, Pradip A., Gomes, James, Bhattacharyay, Sanjoy K., Abdulah, Norhafizah
Format: Article
Language:English
English
Published: Marcel Dekker 1999
Online Access:http://psasir.upm.edu.my/id/eprint/50986/1/50986.pdf
http://psasir.upm.edu.my/id/eprint/50986/7/Production%20of%20Urokinase%20by%20HT%201080%20Human%20Kidney%20cell%20line.pdf
http://psasir.upm.edu.my/id/eprint/50986/
http://www.tandfonline.com/doi/abs/10.3109/10731199909117710
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spelling my.upm.eprints.509862024-08-08T00:44:40Z http://psasir.upm.edu.my/id/eprint/50986/ Production of urokinase by HT 1080 human kidney cell line Roychoudhury, Pradip A. Gomes, James Bhattacharyay, Sanjoy K. Abdulah, Norhafizah Studies were carried out in T-flasks and bioreactor to produce urokinase enzyme using HT 1080 human kidney cell line. While growing the cell line it has been observed that the lag phase is reduced considerably in the bioreactor as compared to T-flask culture. The HT 1080 cell adhesion rate and urokinase production were observed to be the function of serum concentration in the medium. The maximum urokinase activity of 3.1 x 10-4 unit ml-1 was achieved in the bioreactor at around 65 h of batch culture. Since HT 1080 is an anchorage dependent cell line, therefore, the hydrodynamic effects on the cell line were investigated. Marcel Dekker 1999 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/50986/1/50986.pdf text en http://psasir.upm.edu.my/id/eprint/50986/7/Production%20of%20Urokinase%20by%20HT%201080%20Human%20Kidney%20cell%20line.pdf Roychoudhury, Pradip A. and Gomes, James and Bhattacharyay, Sanjoy K. and Abdulah, Norhafizah (1999) Production of urokinase by HT 1080 human kidney cell line. Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, 27 (5&6). pp. 399-402. ISSN 1073-1199 http://www.tandfonline.com/doi/abs/10.3109/10731199909117710 10.3109/10731199909117710
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Studies were carried out in T-flasks and bioreactor to produce urokinase enzyme using HT 1080 human kidney cell line. While growing the cell line it has been observed that the lag phase is reduced considerably in the bioreactor as compared to T-flask culture. The HT 1080 cell adhesion rate and urokinase production were observed to be the function of serum concentration in the medium. The maximum urokinase activity of 3.1 x 10-4 unit ml-1 was achieved in the bioreactor at around 65 h of batch culture. Since HT 1080 is an anchorage dependent cell line, therefore, the hydrodynamic effects on the cell line were investigated.
format Article
author Roychoudhury, Pradip A.
Gomes, James
Bhattacharyay, Sanjoy K.
Abdulah, Norhafizah
spellingShingle Roychoudhury, Pradip A.
Gomes, James
Bhattacharyay, Sanjoy K.
Abdulah, Norhafizah
Production of urokinase by HT 1080 human kidney cell line
author_facet Roychoudhury, Pradip A.
Gomes, James
Bhattacharyay, Sanjoy K.
Abdulah, Norhafizah
author_sort Roychoudhury, Pradip A.
title Production of urokinase by HT 1080 human kidney cell line
title_short Production of urokinase by HT 1080 human kidney cell line
title_full Production of urokinase by HT 1080 human kidney cell line
title_fullStr Production of urokinase by HT 1080 human kidney cell line
title_full_unstemmed Production of urokinase by HT 1080 human kidney cell line
title_sort production of urokinase by ht 1080 human kidney cell line
publisher Marcel Dekker
publishDate 1999
url http://psasir.upm.edu.my/id/eprint/50986/1/50986.pdf
http://psasir.upm.edu.my/id/eprint/50986/7/Production%20of%20Urokinase%20by%20HT%201080%20Human%20Kidney%20cell%20line.pdf
http://psasir.upm.edu.my/id/eprint/50986/
http://www.tandfonline.com/doi/abs/10.3109/10731199909117710
_version_ 1807051111711899648
score 13.211869

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