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Separation of cyclodextrins (CDs) using aqueous two-phase system

Separation of three different types of cyclodextrins (CDs) [α-,β-, and γ- CD] were investigated in the polyethylene glycol (PEG) 20000/ dextran T500 aqueous two-phase system (ATPS) with an aim to study the partitioning behavior of CDs in the absence of the CDs producing enzyme, cyclodextrin glycosyl...

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Main Authors: Ng, Hui Suan, Mokhtar, Mohd Noriznan, Ariff, Arbakariya, Ling, Tau Chuan
Format: Conference or Workshop Item
Language:English
Published: Faculty of Engineering, Universiti Putra Malaysia 2012
Online Access:http://psasir.upm.edu.my/id/eprint/50669/1/_TechnicalPapers_CAFEi2012_104.pdf
http://psasir.upm.edu.my/id/eprint/50669/
http://cafei.upm.edu.my/download.php?filename=/TechnicalPapers/CAFEi2012_104.pdf
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spelling my.upm.eprints.506692017-03-02T06:18:46Z http://psasir.upm.edu.my/id/eprint/50669/ Separation of cyclodextrins (CDs) using aqueous two-phase system Ng, Hui Suan Mokhtar, Mohd Noriznan Ariff, Arbakariya Ling, Tau Chuan Separation of three different types of cyclodextrins (CDs) [α-,β-, and γ- CD] were investigated in the polyethylene glycol (PEG) 20000/ dextran T500 aqueous two-phase system (ATPS) with an aim to study the partitioning behavior of CDs in the absence of the CDs producing enzyme, cyclodextrin glycosyltransferase (CGTase). The ATPS developed (7.7% (w/w) PEG 20000, 10.3% Dextran T500, with volume ratio, VR of 4.0 was the optimum ATPS for the sago starch bioconversion of Bacillus cereus CGTase. This preliminary study was useful for the further kinetic studies of the sago starch conversion into CDs by the CGTase in an ATPS. The study showed that all types of CDs exhibit the PEG-rich phase preference with the high partition coefficient (KCD) over time. However, difference in the partition coefficients between these 3 CDs was observed, suggesting that their partitioning behaviour in the ATPS were different as a result of the difference in the hydrophobic interactions with the phase components and solubility of CDs. The exclusive partition of CDs into the PEG-rich phase is beneficial for the extractive bioconversion of starch in ATPS. Faculty of Engineering, Universiti Putra Malaysia 2012 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/50669/1/_TechnicalPapers_CAFEi2012_104.pdf Ng, Hui Suan and Mokhtar, Mohd Noriznan and Ariff, Arbakariya and Ling, Tau Chuan (2012) Separation of cyclodextrins (CDs) using aqueous two-phase system. In: International Conference on Agricultural and Food Engineering for Life (Cafei2012), 26-28 Nov. 2012, Palm Garden Hotel, Putrajaya. (pp. 319-322). http://cafei.upm.edu.my/download.php?filename=/TechnicalPapers/CAFEi2012_104.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Separation of three different types of cyclodextrins (CDs) [α-,β-, and γ- CD] were investigated in the polyethylene glycol (PEG) 20000/ dextran T500 aqueous two-phase system (ATPS) with an aim to study the partitioning behavior of CDs in the absence of the CDs producing enzyme, cyclodextrin glycosyltransferase (CGTase). The ATPS developed (7.7% (w/w) PEG 20000, 10.3% Dextran T500, with volume ratio, VR of 4.0 was the optimum ATPS for the sago starch bioconversion of Bacillus cereus CGTase. This preliminary study was useful for the further kinetic studies of the sago starch conversion into CDs by the CGTase in an ATPS. The study showed that all types of CDs exhibit the PEG-rich phase preference with the high partition coefficient (KCD) over time. However, difference in the partition coefficients between these 3 CDs was observed, suggesting that their partitioning behaviour in the ATPS were different as a result of the difference in the hydrophobic interactions with the phase components and solubility of CDs. The exclusive partition of CDs into the PEG-rich phase is beneficial for the extractive bioconversion of starch in ATPS.
format Conference or Workshop Item
author Ng, Hui Suan
Mokhtar, Mohd Noriznan
Ariff, Arbakariya
Ling, Tau Chuan
spellingShingle Ng, Hui Suan
Mokhtar, Mohd Noriznan
Ariff, Arbakariya
Ling, Tau Chuan
Separation of cyclodextrins (CDs) using aqueous two-phase system
author_facet Ng, Hui Suan
Mokhtar, Mohd Noriznan
Ariff, Arbakariya
Ling, Tau Chuan
author_sort Ng, Hui Suan
title Separation of cyclodextrins (CDs) using aqueous two-phase system
title_short Separation of cyclodextrins (CDs) using aqueous two-phase system
title_full Separation of cyclodextrins (CDs) using aqueous two-phase system
title_fullStr Separation of cyclodextrins (CDs) using aqueous two-phase system
title_full_unstemmed Separation of cyclodextrins (CDs) using aqueous two-phase system
title_sort separation of cyclodextrins (cds) using aqueous two-phase system
publisher Faculty of Engineering, Universiti Putra Malaysia
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/50669/1/_TechnicalPapers_CAFEi2012_104.pdf
http://psasir.upm.edu.my/id/eprint/50669/
http://cafei.upm.edu.my/download.php?filename=/TechnicalPapers/CAFEi2012_104.pdf
_version_ 1643834731292262400
score 13.211869

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