Enhancement the production of lipase derived from Bukhoderia cenopacia ST8 by optimization of the fermentation operation
A lipase derived from Burkhoderia cenocepacia ST8 was optimized in this research. The experimental design based on ‘change-one-factor-at-a-time’ approach was applied to optimize the production of lipase. Parameters such as volume ratio of medium volume to container volume, concentration of carbon so...
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Faculty of Engineering, Universiti Putra Malaysia
2012
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my.upm.eprints.506542017-03-02T06:11:14Z http://psasir.upm.edu.my/id/eprint/50654/ Enhancement the production of lipase derived from Bukhoderia cenopacia ST8 by optimization of the fermentation operation Show, Pau Loke Anuar, Mohd Shamsul Yusof, Yus Aniza Ariff, Arbakariya Ling, Tau Chuan A lipase derived from Burkhoderia cenocepacia ST8 was optimized in this research. The experimental design based on ‘change-one-factor-at-a-time’ approach was applied to optimize the production of lipase. Parameters such as volume ratio of medium volume to container volume, concentration of carbon source, nutrient broth and metal ions of medium on lipase production were extensively studied. The optimum conditions in cultivation medium containing 1% (w/v) Tween 80, 0.3% (w/v) of nutrient broth and 0.1% (w/v) of CaCl2 with volume ratio 20:80 of medium volume to container volume, a high average production of 43.8 U/mL was obtained. Therefore, the optimum formulations and conditions would produce high yield of lipase were potential utilized in the commercial industrial. Faculty of Engineering, Universiti Putra Malaysia 2012 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/50654/1/_TechnicalPapers_CAFEi2012_37.pdf Show, Pau Loke and Anuar, Mohd Shamsul and Yusof, Yus Aniza and Ariff, Arbakariya and Ling, Tau Chuan (2012) Enhancement the production of lipase derived from Bukhoderia cenopacia ST8 by optimization of the fermentation operation. In: International Conference on Agricultural and Food Engineering for Life (Cafei2012), 26-28 Nov. 2012, Palm Garden Hotel, Putrajaya. (pp. 117-121). http://cafei.upm.edu.my/download.php?filename=/TechnicalPapers/CAFEi2012_37.pdf |
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A lipase derived from Burkhoderia cenocepacia ST8 was optimized in this research. The experimental design based on ‘change-one-factor-at-a-time’ approach was applied to optimize the production of lipase. Parameters such as volume ratio of medium volume to container volume, concentration of carbon source, nutrient broth and metal ions of medium on lipase production were extensively studied. The optimum conditions in cultivation medium containing 1% (w/v) Tween 80, 0.3% (w/v) of nutrient broth and 0.1% (w/v) of CaCl2 with volume ratio 20:80 of medium volume to container volume, a high average production of 43.8 U/mL was obtained. Therefore, the optimum formulations and conditions would produce high yield of lipase were potential utilized in the commercial industrial. |
| format |
Conference or Workshop Item |
| author |
Show, Pau Loke Anuar, Mohd Shamsul Yusof, Yus Aniza Ariff, Arbakariya Ling, Tau Chuan |
| spellingShingle |
Show, Pau Loke Anuar, Mohd Shamsul Yusof, Yus Aniza Ariff, Arbakariya Ling, Tau Chuan Enhancement the production of lipase derived from Bukhoderia cenopacia ST8 by optimization of the fermentation operation |
| author_facet |
Show, Pau Loke Anuar, Mohd Shamsul Yusof, Yus Aniza Ariff, Arbakariya Ling, Tau Chuan |
| author_sort |
Show, Pau Loke |
| title |
Enhancement the production of lipase derived from Bukhoderia cenopacia ST8 by optimization of the fermentation operation |
| title_short |
Enhancement the production of lipase derived from Bukhoderia cenopacia ST8 by optimization of the fermentation operation |
| title_full |
Enhancement the production of lipase derived from Bukhoderia cenopacia ST8 by optimization of the fermentation operation |
| title_fullStr |
Enhancement the production of lipase derived from Bukhoderia cenopacia ST8 by optimization of the fermentation operation |
| title_full_unstemmed |
Enhancement the production of lipase derived from Bukhoderia cenopacia ST8 by optimization of the fermentation operation |
| title_sort |
enhancement the production of lipase derived from bukhoderia cenopacia st8 by optimization of the fermentation operation |
| publisher |
Faculty of Engineering, Universiti Putra Malaysia |
| publishDate |
2012 |
| url |
http://psasir.upm.edu.my/id/eprint/50654/1/_TechnicalPapers_CAFEi2012_37.pdf http://psasir.upm.edu.my/id/eprint/50654/ http://cafei.upm.edu.my/download.php?filename=/TechnicalPapers/CAFEi2012_37.pdf |
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13.211869 |