Cover Image

Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species

The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other than its source species. A total of 25 new microsatellite loci developed for riverine catfish, Mystus n...

Full description

Saved in:
Bibliographic Details
Main Authors: Chan, Soon Choy, Tan, Soon Guan, Siraj, Siti Shapor, Mohd Yusoff, Khatijah
Format: Article
Language:English
Published: Asian-Australasian Association of Animal Production Societies 2005
Online Access:http://psasir.upm.edu.my/id/eprint/50079/1/Newly%20developed%20microsatellite%20markers%20of%20Mystus%20nemurus%20tested%20for%20cross-species%20amplification%20in%20two%20distantly%20related%20aquacultured%20catfish%20species.pdf
http://psasir.upm.edu.my/id/eprint/50079/
http://www.ajas.info/journal/view.php?number=21149
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.50079
record_format eprints
spelling my.upm.eprints.500792017-02-01T04:37:31Z http://psasir.upm.edu.my/id/eprint/50079/ Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species Chan, Soon Choy Tan, Soon Guan Siraj, Siti Shapor Mohd Yusoff, Khatijah The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other than its source species. A total of 25 new microsatellite loci developed for riverine catfish, Mystus nemurus were successfully cross-amplified in two distantly related catfish species within the suborder Siluroidei. Five out of the 19 loci that successfully cross-amplified in Pangasius micronemus were polymorphic, while for Clarias batrachus, cross-amplification was successful using 17 polymorphic loci. The observed heterozygosities were high for all the three catfishes. The results indicated that microsatellite loci could be as polymorphic in non-source species as in the source species. Asian-Australasian Association of Animal Production Societies 2005 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/50079/1/Newly%20developed%20microsatellite%20markers%20of%20Mystus%20nemurus%20tested%20for%20cross-species%20amplification%20in%20two%20distantly%20related%20aquacultured%20catfish%20species.pdf Chan, Soon Choy and Tan, Soon Guan and Siraj, Siti Shapor and Mohd Yusoff, Khatijah (2005) Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species. Asian-Australasian Journal of Animal Sciences, 18 (11). pp. 1513-1518. ISSN 1011-2367; ESSN: 1976-5517 http://www.ajas.info/journal/view.php?number=21149 10.5713/ajas.2005.1513
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other than its source species. A total of 25 new microsatellite loci developed for riverine catfish, Mystus nemurus were successfully cross-amplified in two distantly related catfish species within the suborder Siluroidei. Five out of the 19 loci that successfully cross-amplified in Pangasius micronemus were polymorphic, while for Clarias batrachus, cross-amplification was successful using 17 polymorphic loci. The observed heterozygosities were high for all the three catfishes. The results indicated that microsatellite loci could be as polymorphic in non-source species as in the source species.
format Article
author Chan, Soon Choy
Tan, Soon Guan
Siraj, Siti Shapor
Mohd Yusoff, Khatijah
spellingShingle Chan, Soon Choy
Tan, Soon Guan
Siraj, Siti Shapor
Mohd Yusoff, Khatijah
Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species
author_facet Chan, Soon Choy
Tan, Soon Guan
Siraj, Siti Shapor
Mohd Yusoff, Khatijah
author_sort Chan, Soon Choy
title Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species
title_short Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species
title_full Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species
title_fullStr Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species
title_full_unstemmed Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species
title_sort newly developed microsatellite markers of mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species
publisher Asian-Australasian Association of Animal Production Societies
publishDate 2005
url http://psasir.upm.edu.my/id/eprint/50079/1/Newly%20developed%20microsatellite%20markers%20of%20Mystus%20nemurus%20tested%20for%20cross-species%20amplification%20in%20two%20distantly%20related%20aquacultured%20catfish%20species.pdf
http://psasir.upm.edu.my/id/eprint/50079/
http://www.ajas.info/journal/view.php?number=21149
_version_ 1643834556874227712
score 13.211869

Similar Items