Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent

Due to increasing awareness of the risk of the environmental pollution and emission of green house gases (GHG) that caused global warming, a study of palm oil mill effluent (POME) treatment using biological processes in close digesters has been conducted. Potentially beneficial methane gas productio...

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Main Author: Zakaria @ Mamat, Mohd Rafein
Format: Thesis
Language:English
English
Published: 2008
Online Access:http://psasir.upm.edu.my/id/eprint/4935/1/FBSB_2008_9.pdf
http://psasir.upm.edu.my/id/eprint/4935/
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spelling my.upm.eprints.49352013-05-27T07:19:14Z http://psasir.upm.edu.my/id/eprint/4935/ Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent Zakaria @ Mamat, Mohd Rafein Due to increasing awareness of the risk of the environmental pollution and emission of green house gases (GHG) that caused global warming, a study of palm oil mill effluent (POME) treatment using biological processes in close digesters has been conducted. Potentially beneficial methane gas production from this treatment has driven the objectives of this study to explore the methanogens from POME sludge and their characteristic in order to improve the POME treatment efficiency. A pilot plant digester with 500 m3 volume was operated for one year and biogas and methane production profiles were monitored daily. Parameters used for monitoring were pH, temperature, volatile fatty acids, chemical oxygen demand, biogas and methane concentration during the treatment. Structure of the flocs formation and methanogenic bacteria isolated from this digester was also carried out in order to understand the microbiological characteristics of the bioprocess involved Specific methanogenic activity test (SMA) and microscopic observation were carried out to support the existence of the methanogens that were able to produce methane gas. During POME treatment process, the highest methane concentration obtained was 55 % (v/v) and chemical oxygen demand (COD) removal efficiency was up to 95%. Anticipated active biomasses retained in the digester were determined as total solids (TS) and volatile suspended solids (VSS) at 2.5% and 1.5%, respectively. SMA test for acetolastic methanogens was determined in the range of 0.05 - 1.3 g COD/ g VSS/d. Microscopic observation of the sludge and isolated colonies have shown that the digester systems were dominated by microorganism resemble to Methanosaeta sp. and fluorescence microscope has proved that this microorganism exhibited autofluorescence green color. 2008 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/4935/1/FBSB_2008_9.pdf Zakaria @ Mamat, Mohd Rafein (2008) Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent. Masters thesis, Universiti Putra Malaysia. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Due to increasing awareness of the risk of the environmental pollution and emission of green house gases (GHG) that caused global warming, a study of palm oil mill effluent (POME) treatment using biological processes in close digesters has been conducted. Potentially beneficial methane gas production from this treatment has driven the objectives of this study to explore the methanogens from POME sludge and their characteristic in order to improve the POME treatment efficiency. A pilot plant digester with 500 m3 volume was operated for one year and biogas and methane production profiles were monitored daily. Parameters used for monitoring were pH, temperature, volatile fatty acids, chemical oxygen demand, biogas and methane concentration during the treatment. Structure of the flocs formation and methanogenic bacteria isolated from this digester was also carried out in order to understand the microbiological characteristics of the bioprocess involved Specific methanogenic activity test (SMA) and microscopic observation were carried out to support the existence of the methanogens that were able to produce methane gas. During POME treatment process, the highest methane concentration obtained was 55 % (v/v) and chemical oxygen demand (COD) removal efficiency was up to 95%. Anticipated active biomasses retained in the digester were determined as total solids (TS) and volatile suspended solids (VSS) at 2.5% and 1.5%, respectively. SMA test for acetolastic methanogens was determined in the range of 0.05 - 1.3 g COD/ g VSS/d. Microscopic observation of the sludge and isolated colonies have shown that the digester systems were dominated by microorganism resemble to Methanosaeta sp. and fluorescence microscope has proved that this microorganism exhibited autofluorescence green color.
format Thesis
author Zakaria @ Mamat, Mohd Rafein
spellingShingle Zakaria @ Mamat, Mohd Rafein
Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent
author_facet Zakaria @ Mamat, Mohd Rafein
author_sort Zakaria @ Mamat, Mohd Rafein
title Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent
title_short Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent
title_full Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent
title_fullStr Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent
title_full_unstemmed Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent
title_sort biogas production and determination of methanogens from digester - treated palm oil mill effluent
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/4935/1/FBSB_2008_9.pdf
http://psasir.upm.edu.my/id/eprint/4935/
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score 13.211869