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Cymbidium mosaic virus and odontoglossum ringspot tobamovirus genes cloned from infected Oncidium orchids

Several recombinant phages were picked at random from the cDNA library of Oncidium (Oncidium Goldiana x Oncidium Flexuosum) flowers, converted into plasmids by in vivo excision and sequenced. Two of the clones named CyMV1 and CyMV2, showed very high DNA and protein sequence homology to those of the...

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Main Authors: Mohamed Bahari, Umikalsum, Abu Bakar, Umi Kalsom, Nasir, Khairun Hisam, Qamaruz Zaman, Faridah
Format: Article
Language:English
Published: Malaysian Agricultural Research and Development Institute (MARDI) 2006
Online Access:http://psasir.upm.edu.my/id/eprint/48943/1/Cymbidium%20mosaic%20virus%20and%20odontoglossum%20ringspot%20tobamovirus%20genes%20cloned%20from%20infected%20Oncidium%20orchids.pdf
http://psasir.upm.edu.my/id/eprint/48943/
http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/41-2006/volume-34-no1/185-340115
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spelling my.upm.eprints.489432016-12-30T02:25:57Z http://psasir.upm.edu.my/id/eprint/48943/ Cymbidium mosaic virus and odontoglossum ringspot tobamovirus genes cloned from infected Oncidium orchids Mohamed Bahari, Umikalsum Abu Bakar, Umi Kalsom Nasir, Khairun Hisam Qamaruz Zaman, Faridah Several recombinant phages were picked at random from the cDNA library of Oncidium (Oncidium Goldiana x Oncidium Flexuosum) flowers, converted into plasmids by in vivo excision and sequenced. Two of the clones named CyMV1 and CyMV2, showed very high DNA and protein sequence homology to those of the cymbidium mosaic virus (CyMV) in the genebank database. CyMV1, 1,186 bp in size, contained within it the entire sequence for coat protein (CP) gene, movement protein (MP)3 gene and an almost complete sequence for MP2 gene. CyMV2, which is 626 bp in size, only contained the extreme 3’ end sequence of the RNA polymerase gene. The percentage of homology of the isolated CyMV1 gene was 97% to the Taiwanese strain (AY571289), 96% to the Korean type 2 CyMV complete genome (AF016914) and to the Singaporean CyMV complete genome (CMU62963) in the CP and MP regions of the genome. CyMV2 showed 95% homology to the Korean type 2 CyMV complete genome (AF016914) and to the Singaporean CyMV complete genome (CMU62963) but in the RNA polymerase region. Another clone named ORSV1, 728 bp in size, isolated by RT-PCR method was a partial fragment of odontoglossum ringspot virus (ORSV) RNA replicase gene. This partial gene sequence of ORSV1 showed 98% homology to the ORSV gene isolated from United States (Accession nos. ORU89894), Taiwan (Accession nos. AY571290) and Korea (Accession nos. X82130). All of these genes could be used in developing Oncidium orchids resistant to CyMV or ORSV through the transgenic approach. Malaysian Agricultural Research and Development Institute (MARDI) 2006 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/48943/1/Cymbidium%20mosaic%20virus%20and%20odontoglossum%20ringspot%20tobamovirus%20genes%20cloned%20from%20infected%20Oncidium%20orchids.pdf Mohamed Bahari, Umikalsum and Abu Bakar, Umi Kalsom and Nasir, Khairun Hisam and Qamaruz Zaman, Faridah (2006) Cymbidium mosaic virus and odontoglossum ringspot tobamovirus genes cloned from infected Oncidium orchids. Journal of Tropical Agriculture and Food Science, 34 (1). pp. 139-148. ISSN 1394-9829; ESSN: 2289-9650 http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/41-2006/volume-34-no1/185-340115
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Several recombinant phages were picked at random from the cDNA library of Oncidium (Oncidium Goldiana x Oncidium Flexuosum) flowers, converted into plasmids by in vivo excision and sequenced. Two of the clones named CyMV1 and CyMV2, showed very high DNA and protein sequence homology to those of the cymbidium mosaic virus (CyMV) in the genebank database. CyMV1, 1,186 bp in size, contained within it the entire sequence for coat protein (CP) gene, movement protein (MP)3 gene and an almost complete sequence for MP2 gene. CyMV2, which is 626 bp in size, only contained the extreme 3’ end sequence of the RNA polymerase gene. The percentage of homology of the isolated CyMV1 gene was 97% to the Taiwanese strain (AY571289), 96% to the Korean type 2 CyMV complete genome (AF016914) and to the Singaporean CyMV complete genome (CMU62963) in the CP and MP regions of the genome. CyMV2 showed 95% homology to the Korean type 2 CyMV complete genome (AF016914) and to the Singaporean CyMV complete genome (CMU62963) but in the RNA polymerase region. Another clone named ORSV1, 728 bp in size, isolated by RT-PCR method was a partial fragment of odontoglossum ringspot virus (ORSV) RNA replicase gene. This partial gene sequence of ORSV1 showed 98% homology to the ORSV gene isolated from United States (Accession nos. ORU89894), Taiwan (Accession nos. AY571290) and Korea (Accession nos. X82130). All of these genes could be used in developing Oncidium orchids resistant to CyMV or ORSV through the transgenic approach.
format Article
author Mohamed Bahari, Umikalsum
Abu Bakar, Umi Kalsom
Nasir, Khairun Hisam
Qamaruz Zaman, Faridah
spellingShingle Mohamed Bahari, Umikalsum
Abu Bakar, Umi Kalsom
Nasir, Khairun Hisam
Qamaruz Zaman, Faridah
Cymbidium mosaic virus and odontoglossum ringspot tobamovirus genes cloned from infected Oncidium orchids
author_facet Mohamed Bahari, Umikalsum
Abu Bakar, Umi Kalsom
Nasir, Khairun Hisam
Qamaruz Zaman, Faridah
author_sort Mohamed Bahari, Umikalsum
title Cymbidium mosaic virus and odontoglossum ringspot tobamovirus genes cloned from infected Oncidium orchids
title_short Cymbidium mosaic virus and odontoglossum ringspot tobamovirus genes cloned from infected Oncidium orchids
title_full Cymbidium mosaic virus and odontoglossum ringspot tobamovirus genes cloned from infected Oncidium orchids
title_fullStr Cymbidium mosaic virus and odontoglossum ringspot tobamovirus genes cloned from infected Oncidium orchids
title_full_unstemmed Cymbidium mosaic virus and odontoglossum ringspot tobamovirus genes cloned from infected Oncidium orchids
title_sort cymbidium mosaic virus and odontoglossum ringspot tobamovirus genes cloned from infected oncidium orchids
publisher Malaysian Agricultural Research and Development Institute (MARDI)
publishDate 2006
url http://psasir.upm.edu.my/id/eprint/48943/1/Cymbidium%20mosaic%20virus%20and%20odontoglossum%20ringspot%20tobamovirus%20genes%20cloned%20from%20infected%20Oncidium%20orchids.pdf
http://psasir.upm.edu.my/id/eprint/48943/
http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/41-2006/volume-34-no1/185-340115
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score 13.211869

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